... portion precursor map in anterior epiblasts at st. 4/5. Grafting outside the brain precursor region of mCherry-expressing nodes producing anterior mesendoderm (AME) or isolated AME tissues elicited new cell migration towards ectopic AME tissues. These locally convergent cells developed into secondary...

...Yoshihiro Komatsu; Vesa Kaartinen; Yuji Mishina Cilia at the node generate a leftward fluid flow that breaks left-right symmetry. However, the molecular mechanisms that regulate ciliogenesis at the node are largely unknown. Here, we show that the epiblast-specific deletion of the gene encoding...

...Shinya Oki; Keiko Kitajima; Sara Marques; José António Belo; Takahiko Yokoyama; Hiroshi Hamada; Chikara Meno The node at the anterior tip of the primitive streak serves as an initial generator of the left-right (L-R) axis in mammalian embryos. We now show that a small disturbance in molecular...

... of e-cadherin , thereby perturbing cell movements during convergent extension, epiboly and node formation. Thus, although the role of Smad proteins in mediating Nodal signaling is well-documented, the functional characterization of Ttrap provides insight into a novel Smad partner that plays...

...Shinya Oki; Ryuju Hashimoto; Yuko Okui; Michael M. Shen; Eisuke Mekada; Hiroki Otani; Yukio Saijoh; Hiroshi Hamada Situs-specific organogenesis in the mouse results from leftward fluid flow in the node cavity and subsequent left-sided expression of Nodal in the lateral plate mesoderm (LPM). Nodal...

..., the chordoneural hinge, but the localisation of such progenitors at earlier stages is so far untested. By studying gene expression, we have shown that a specific topological arrangement of domains persists from the streak to the tail bud, and includes an area (the node-streak border) in which ectoderm...

... regulated by the coordination of five phylogenetically conserved enhancers having discrete regional coverage, among which the 420-bp long enhancer N-1, active in the node-proximal region, is probably involved directly in the genesis of the posterior neural plate. We investigated the signaling systems...

...Atsushi Sawada; Yuriko Nishizaki; Hiroko Sato; Yukari Yada; Rika Nakayama; Shinji Yamamoto; Noriyuki Nishioka; Hisato Kondoh; Hiroshi Sasaki The cell population and the activity of the organizer change during the course of development. We addressed the mechanism of mouse node development via...

... requirement for a repressor function of Lef/Tcf proteins during early mouse development. Tcf3 -/- embryos proceed through gastrulation to form mesoderm, but they develop expanded and often duplicated axial mesoderm structures, including nodes and notochords. These duplications are preceded by ectopic...

... was required for repression in regions of the CNS where Shh is not normally expressed. We further show that Shh enhancer activity was detected in the mouse node from where the floor plate and notochord precursors derive. Shh reporter expression was restricted to the ventral (mesodermal)layer of the node...

... that the fusion protein is functional. In transgenic embryos, Inv::GFP protein was detected in the node monocilia. The fusion protein was also present in other 9+0 monocilia,including those of kidney epithelial cells and the pituitary gland, but it was not localized to 9+2 cilia. The N-terminal region of Inv...

...Takeshi Fujiwara; Deborah B. Dehart; Kathleen K. Sulik; Brigid L. M. Hogan In the mouse and chick embryo, the node plays a central role in generating left-right (LR) positional information. Using several different strategies, we provide evidence in the mouse that bone morphogenetic protein 4 (Bmp4...

... in epiblast and nascent mesoderm of the primitive streak but not in Hensen’s node. Cmix transcripts are no longer detectable after formation of the head process (Peale et al., 1998 ; Stein et al., 1998). In zebrafish, the gene associated with the bonnie and clyde (Bon) mutation is a Mix -type gene...

... , and the ability of these cells to induce a second neural axis in the host embryo. This cell population can therefore be regarded as the mid-gastrula organizer and, together with the early-gastrula organizer and the node, constitute the organizer of the mouse embryo at successive stages of development. The profile...

...) * Present address: Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan 23 02 2001 19 04 2001 © 2001 by Company of Biologists 2001 nodal Anteroposterior axis Left-right asymmetry Node Lateral plate...

... mesoderm. In the first domain of expression, Cryptic expression is progressively localized to the anterior primitive streak, the head process, and the node and notochordal plate. In the second domain, Cryptic expression is initially concentrated in the lateral region of the egg cylinder, and is later found...

... 1996 Otx2 neural tube patterning gastrulation notochord prechordal mesoderm node gene targeting The patterning and development of the vertebrate neural tube is a complex process involving both cell extrinsic and cell intrinsic events. Among the cell extrinsic events...

... that different post-transcriptional mechanisms control Otx2 expression in both layers. * Author for correspondence † Both first authors contributed equally to the work 27 6 1995 © 1995 by Company of Biologists 1995 neural induction Otx2 mouse gastrulation node anterior...

... is detected in eed mutant embryos, and there is no subsequent organization of mesoderm into node, notochord, or somites. The phenotype is consistent with a defect in the distal primitive streak. Here we report additional phenotypic analyses that include mRNA in situ hybridization of genes whose expression...

..., until now there has been no direct evidence that the mouse node organizes pattern during gastrulation, nor that the exceptionally small mouse embryonic egg cylinder can be induced to form a second axis. Grafts of transgenically marked mid-gastrulation mouse node, or node labelled with DiI...