... in epiboly using the microtubule depolymerizing agent nocodazole and a stabilizing agent taxol. In embryos treated with nocodazole, microtubules were absent and epibolic movements of the yolk syncytial nuclei were blocked. In contrast, the vegetal expansion of the enveloping layer and deep cells was only...

... of nocodazole showed that this continuing polymerisation does not contribute to the rotation. Concentrations of the drug that prevented tubulin polymerisation had no effect on the speed of rotation if applied after the vegetal array had formed. The same doses prevented movement if applied early enough...

... through mitosis. Whereas in most late interphase cells microvilli were restricted to a discrete polar region, in mitotic cells at all stages from early metaphase to immediately postcytokinesis microvilli were found to be present over more of the cell surface. Other mitotic cells were placed in nocodazole...

... other), flattened (folds parted from 90° to 180°) or collapsed (folds parted more than 180°). The microtubule inhibitors colchicine and nocodazole at 10 −4 M and 6·6×10 −4 M respectively cause the cephalic neural folds of 10·4-day embryos to collapse after 60 min. At 5·2×10 −6 M the microfilament...

...Jonathan Van Blerkom; Hobart Bell ABSTRACT The relationship between nuclear maturation and the differentiation of the cytoplasm and plasma membrane during resumption of arrested meiosis was investigated by culture of GV- and Mil-stage mouse oocytes in the presence and absence of nocodazole. Culture...

..., nocodazole, cytochalasin D or in a combination of nocodazole plus cytochalasin D for different parts of the incubation period was examined. Neither the development nor the stability of the surface polarity in 8-cell blastomeres was blocked by any treatment and only the use of CCD in combination...