... transcription factor is a central regulator of cardiac development and is expressed in both the first and second heart fields (FHF and SHF). We have previously revealed essential functions of nkx2.5 and nkx2.7 , two Nkx2-5 homologs expressed in zebrafish cardiomyocytes, in maintaining ventricular identity...

... outflow tract (OFT). In zebrafish, previous lineage-tracing and mutant analyses suggested that SHF ventricular and OFT progenitors co-migrate to the arterial pole of the zebrafish heart tube soon after their specification in the nkx2.5 + field of anterior lateral plate mesoderm (ALPM). Using additional...

... and inhibiting atrial gene expression via Nkx factors. Atrium Ventricle Amhc Vmhc Nkx2.5 Zebrafish The vertebrate heart is divided into atrial and ventricular chambers that work together to drive circulation, yet have unique morphological, electrophysiological and contractile attributes...

... and maintaining unique features in ventricular and atrial cardiomyocytes, the regulatory mechanisms guiding these processes are poorly understood. Here, we show that the homeodomain transcription factors Nkx2.5 and Nkx2.7 are necessary to sustain ventricular chamber attributes through repression of atrial chamber...

... lineage traced gata4 + and nkx2.5 + ALPM populations predicted to contain SHF progenitors, based on evolutionary conservation of ALPM patterning. Traced cells were identified in SHF-derived distal ventricular myocardium and in three lineages in the outflow tract (OFT). We confirmed the extent...

... be via suppression of the haemangioblast programme. However, we show that the cardiac regulator nkx2.5 can also repress the haemangioblast programme and, furthermore, that cardiac specification still requires Fgf signalling even when haemangioblast regulators are independently suppressed. We further show...

...Stéphane Zaffran; Ingolf Reim; Li Qian; Patrick C. Lo; Rolf Bodmer; Manfred Frasch The NK homeobox gene tinman ( tin ) is required for the specification of the cardiac, visceral muscle and somatic muscle progenitors in the early dorsal mesoderm of Drosophila. Like its vertebrate counterpart Nkx2.5...

... the synergistic activity of key cardiac transcription factors, Nkx2.5 and Tbx5, leading to reduced activation of the common target gene, Anf ( Nppa ). Strikingly, reduced levels of Anf expression were also observed in embryonic day 9.5 Carp-Lbh transgenic mice. Thus, repression of Nkx2.5 and Tbx5-mediated gene...

... (Tlx1), Nkx3.2 (Bapx1) and Pod1 (capsulin, Tcf21). We show that Hox11 and Nkx2.5 , among the earliest known markers for splenic progenitor cells, are absent in the splenic anlage of Pbx1 homozygous mutant ( -/- ) embryos, implicating the TALE homeoprotein Pbx1 in splenic cell specification. Pbx1...

... of key cardiac regulators, we have characterized the cis-regulatory elements of the chick tinman homolog chick Nkx2.5 . We find that at least three distinct cardiac activating regions (CARs) of chick Nkx2.5 cooperate to regulate early expression in the cardiac crescent and later segmental expression...

... required for maintaining normal expression levels of several key cardiogenic factors (e.g. NKX2.5 and MEF2C) in the developing myocardium at mid-gestation. Furthermore, BMP10-conditioned medium is able to rescue BMP10-deficient hearts in culture. Our data suggest an important pathway that involves...

... drawn in studies based on these maps. We present accurate fate maps of the heart-forming region (HFR) in avian embryos and show that the putative cardiogenic molecular markers Bmp2 and Nkx2.5 do not govern the boundaries of the HFR as suggested in the literature. Moreover, this paper presents the first...

... find that Nkx2.5 is necessary and sufficient for establishing aspects of pyloric sphincter differentiation. * Author for correspondence (e-mail: tabin@rascal.med.harvard.edu) 09 06 2000 09 08 2000 © 2000 by Company of Biologists 2000 BMP4 BMP receptor Nkx2.5 Gut...

... for induction and patterning of myocardial precursors. * Author for correspondence (e-mail: brand@sun0.urz.uni-heidelberg.de ) 01 11 1999 © 2000 by Company of Biologists 2000 Cardiogenesis Heart Ventricle fgf8 acerebellar nkx2.5 gata4 BMP Zebrafish ( Danio rerio...

... Nkx2-5 ( Reecy et al., 1999 ). * Author for correspondence (e-mail: eolson@hamon.swmed.edu ) 20 07 1999 07 09 1999 © 1999 by Company of Biologists 1999 Heart Transcription cis -element Nkx2.5 The heart is the first organ to form in mammals. Cardiogenic...

... upstream regulatory region of the gene and cooperative transcriptional regulation mediated by Nkx2.5 and GATA-4. We have shown that this cooperative regulation is dependent on binding of GATA-4 to its cognate DNA sequence in the promoter, which suggests that Nkx2.5 controls CARP expression, at least...

... that the prechordal plate supports and/or the notochord suppresses the cardiac fate. * Author for correspondence (e-mail: fishman@cvrc.mgh.harvard.edu ) 17 02 1998 08 01 1998 © 1998 by Company of Biologists 1998 Regulation Heart field Zebrafish Nkx2.5 GATA 4 Cell fate...