...Dina Rekler; Shai Ofek; Sarah Kagan; Gilgi Friedlander; Chaya Kalcheim Dorsal neural tube-derived retinoic acid promotes the end of neural crest production and transition into a definitive roof plate. Here we analyze how this impacts the segregation of central and peripheral lineages, a process...

...Eirini Maniou; Faduma Farah; Abigail R. Marshall; Zoe Crane-Smith; Andrea Krstevski; Athanasia Stathopoulou; Nicholas D. E. Greene; Andrew J. Copp; Gabriel L. Galea ABSTRACT Closed spinal dysraphisms are poorly understood malformations classified as neural tube (NT) defects. Several, including...

... the response to retinoic acid. Hox genes Transcriptional regulation Shared enhancers Nascent transcription smFISH Retinoic acid signaling Retinoic acid response elements RAREs Neural tube Mouse development Stowers Institute for Medical Research http://dx.doi.org/10.13039/100007795...

...JiSoo Park; Hao-An Hsiung; Irina Khven; Gioele La Manno; Matthias P. Lutolf ABSTRACT The embryonic neural tube is the origin of the entire adult nervous system, and disturbances in its development cause life-threatening birth defects. However, the study of mammalian neural tube development...

...× Minimum Essential Medium (MEM; Gibco), 1× GlutaMax (Gibco) and neutralizing bicarbonate (Gibco)]. Four neural tube-containing collagen drops (5 µl) were distributed on a 35 mm glass-bottom culture dish (ibidi) (procedure modified from Das et al., 2012 ). Collagen polymerization was performed at 38°C...

.... A. , Elder , N. H. , Bulger , E. A. , Krakora , M. Z. , Gaylord , E. A. , Mendoza-Camacho , F. , Butts , J. C. and McDevitt , T. C. ( 2021 ). Axial elongation of caudalized human organoids mimics aspects of neural tube development . Development 148 , dev198275 . 10.1242...

... the sharp boundaries that delineate these gene expression patterns arise, despite the stochasticity associated with gene regulation, is poorly understood. We show, in the vertebrate neural tube, using perturbations of coding and regulatory regions, that the structure of the regulatory network contributes...

...Andrew C. Symonds; Clare E. Buckley; Charlotte A. Williams; Jonathan D. W. Clarke ABSTRACT Using the zebrafish neural tube as a model, we uncover the in vivo mechanisms allowing the generation of two opposing apical epithelial surfaces within the centre of an initially unpolarised, solid organ. We...

.... By investigating loss and gain of Shh function, and floor-plate deletions, we report that sclerotomal Shh is also necessary for neural tube development. Reducing the amount of Shh in the sclerotome using a membrane-tethered hedgehog-interacting protein or Patched1, but not dominant active Patched, decreased...

...Pilar Guerrero; Ruben Perez-Carrasco; Marcin Zagorski; David Page; Anna Kicheva; James Briscoe; Karen M. Page ABSTRACT Cell division, movement and differentiation contribute to pattern formation in developing tissues. This is the case in the vertebrate neural tube, in which neurons differentiate...

... Neural tube Neurogenesis Neuronal diversity Patterning The spinal cord is crucial for how we perceive and interact with our environment. Perception of sensory information from our skin, muscles and internal organs depends on the activities of several classes of neurons, the cell bodies...

...Atsuki Yatsuzuka; Akiko Hori; Minori Kadoya; Mami Matsuo-Takasaki; Toru Kondo; Noriaki Sasai ABSTRACT Dorsal-ventral pattern formation of the neural tube is regulated by temporal and spatial activities of extracellular signalling molecules. Sonic hedgehog (Shh) assigns ventral neural subtypes via...

...Julien Delile; Teresa Rayon; Manuela Melchionda; Amelia Edwards; James Briscoe; Andreas Sagner; Allon Klein; Barbara Treutlein ABSTRACT The coordinated spatial and temporal regulation of gene expression in the vertebrate neural tube determines the identity of neural progenitors and the function...

...Evanthia Nikolopoulou; Gabriel L. Galea; Ana Rolo; Nicholas D. E. Greene; Andrew J. Copp Neural tube closure has been studied for many decades, across a range of vertebrates, as a paradigm of embryonic morphogenesis. Neurulation is of particular interest in view of the severe congenital...

... µg/ml netrin 1 (R&D Systems), 0.5 mM L-glutamine (Gibco), 50 U/ml penicillin (Gibco) and 50 µg/ml streptomycin (Gibco). Wild-type and mutant mouse embryos (E13.5) were collected in cold PBS and dissected in cold Hank's Balanced Salt Solution (HBSS), 6.5% glucose (Gibco). Neural tubes were...

...Joaquim Grego-Bessa; Jeffrey Hildebrand; Kathryn V. Anderson The genetic control of mammalian epithelial polarity and dynamics can be studied in vivo at cellular resolution during morphogenesis of the mouse neural tube. The mouse neural plate is a simple epithelium that is transformed...

... migration has not been studied in mammalian PCP mutants. Vangl2 Lp/Lp mouse embryos lack PCP signalling and undergo almost complete failure of neural tube closure. Here we show, however, that NC specification, migration and derivative formation occur normally in Vangl2 Lp/Lp embryos. The gene family member...

... removal of PTCH2- and PTCH1-feedback antagonism produces a significant expansion of HH-dependent ventral neural progenitors. Strikingly, complete loss of PTCH2-, HHIP1- and PTCH1-feedback inhibition results in ectopic specification of ventral cell fates throughout the neural tube, reflecting constitutive...

... downregulation in the dorsal neural tube is thus necessary for the switch between neural and melanocytic phases of NC development. * Present address: Program in Genomics of Differentiation, National Institute of Child Health and Human Development, 6B/3B309, Bethesda, MD 20892, USA ‡ Author...

... differential gene expression. However, this view has been challenged, raising the possibility that distinct mechanisms are used to interpret different morphogens. Here, we investigate graded BMP signalling in the vertebrate neural tube. We show that defined exposure times to Bmp4 generate distinct levels...