... whether ectopic expression of E2A can rescue the E2A KO early neural defect, we established cell lines stably expressing E2A in E2A KO hESCs via lentiviral transduction (Lv-E2A). We then induced neural differentiation of wild-type, E2A KO and Lv-E2A (∼99% GFP + ) hESCs, and western blot analysis...

... development in vitro and brain development in vivo . These topics were discussed extensively at a recent workshop organized by The Company of Biologists entitled ‘Thinking beyond the dish: taking in vitro neural differentiation to the next level’. Here, we summarize the common themes that emerged from...

... of migration. Taken together, these data suggest that Prdm16 plays an important role in dynamic cellular redox changes in developing neocortex during neural differentiation. To determine endogenous mtROS levels in neocortical cells, we performed fluorescence-activated cell sorting (FACS) using...

.... , Varela , C. , Bonnefond , C. , Maury , Y. , Bugi , A. , Aubry , L. , Viegas , P. , Bourgois-Rocha , F. , Peschanski , M. and Perrier , A. L. ( 2013 ). Embryonic stem cells neural differentiation qualifies the role of Wnt/beta-Catenin signals in human telencephalic...

...Wanqu Zhu; Xiao Yao; Yan Liang; Dan Liang; Lu Song; Naihe Jing; Jinsong Li; Gang Wang Unraveling the mechanisms underlying early neural differentiation of embryonic stem cells (ESCs) is crucial to developing cell-based therapies of neurodegenerative diseases. Neural fate acquisition is proposed...

...Isabel Olivera-Martinez; Nick Schurch; Roman A. Li; Junfang Song; Pamela A. Halley; Raman M. Das; Dave W. Burt; Geoffrey J. Barton; Kate G. Storey Here, we exploit the spatial separation of temporal events of neural differentiation in the elongating chick body axis to provide the first analysis...

... blinded to the experimental conditions. Three to four coverslips in each group were counted. Data are expressed as mean±s.d. Total RNA was extracted from hESCs and neural differentiation cultures using RNA STAT-60 (Tel-Test, Friendswood, TX, USA). cDNA was synthesized using the SuperScript III...

... arnt2 sim1 Neural differentiation © 2009. 2009 26 1 2009 * Present address: Max Planck Institute for Medical Research, Jahnstrasse 29,69120 Heidelberg, Germany † Author for correspondence (e-mail: driever@biologie.uni-freiburg.de ) ...

... proliferate before they exit from the cell cycle and differentiate into neural cells. However,little is known about the relationship between cell division and neural differentiation, although, in Xenopus , cell division after the onset of gastrulation has been suggested to be nonessential for neural...

... CpG methylation Neural differentiation STAT1 Chromatin remodeling MeCP2 Histone modification Mouse CpG DNA methylation is one of the major epigenetic factors that influences gene expression in mammals ( Jaenisch and Bird, 2003 ). DNA methylation is essential for embryogenesis as mice...

.... † Author for correspondence (e-mail: hbaier@itsa.ucsf.edu ) * These authors contributed equally to this work 28 11 2003 © 2004. 2004 Retina Neural differentiation Axon guidance Target selection Zebrafish Neural circuits with specific functions are frequently organized...

... resulted in increased proliferation of neural plate cells, causing expansion of the neural field followed by neural tube and craniofacial abnormalities. Overexpressed TH protein repressed neural differentiation and neural crest markers, but did not inhibit the neural inducers, pan-neural markers...

... by cell position with respect to the ventral midline and controlled by polarizing signals that originate from the floor plate and notochord. Key words: dorsoventral patterning, floor plate, notochord, neural differentiation, spinal cord. Introduction A basic problem in vertebrate development is to define...