... to promote dorsal mesoderm formation, but their role in myogenesis has been unclear. Using zebrafish, we show that dorsally derived Fgf signals act through Tbx16 and Tbxta to induce slow and fast trunk muscle precursors at distinct dorsoventral positions. Tbx16 binds to and directly activates the myf5...

... http://www.biologists.com/user-licence-1-1/ Summary: Analysis of PDGFRα gain-of-function mutant mice demonstrates a role in balancing the expansion of fibroblast and adipocyte lineages before birth. Platelet-derived growth factor Adipocyte Lipodystrophy Myf5 Cell fate Mouse...

... a differential role during both the patterning and the growth phases. Analysis of Tg(GBS-GFP) mice in conjunction with Shh and Gli3 mutants during the patterning phase provides evidence that two myogenic subpopulations co-exist in the epaxial myotome. First, myoblasts expressing Myf5 but devoid of GBS-GFP...

... developmental cues produce distinct phenotypic classes of myoblasts. Notch Skeletal muscle Spatiotemporal differentiation Stem cells Pax7 Myf5 Rbpj Nfix How a continuum of founder stem cells generates distinct phenotypic classes of differentiating cells during organogenesis...

...Natalia Moncaut; Joe W. Cross; Christine Siligan; Annette Keith; Kevin Taylor; Peter W. J. Rigby; Jaime J. Carvajal The specification of the skeletal muscle lineage during craniofacial development is dependent on the activity of MYF5 and MYOD, two members of the myogenic regulatory factor family...

... and differentiation into fibers, demonstrating that regulation of pioneer patterning is somite-intrinsic. Furthermore, pioneer myoblasts express Robo2 downstream of MyoD and Myf5, whereas the dermomyotome and caudal sclerotome express Slit1. Loss of Robo2 or of sclerotome-derived Slit1 function perturbed both...

... containing the equivalent amount of buffer (5 mM Tris-HCl,150 mM NaCl, 2 mM EDTA). * Author for correspondence( [email protected] ) 17 8 2009 © 2009. 2009 Laminin Myf5 Sonic hedgehog Basement membrane Myogenesis Myotome Mouse Skeletal muscles originate...

...Yaniv Hinits; Daniel P. S. Osborn; Simon M. Hughes Myogenic regulatory factors of the Myod family (MRFs) are transcription factors essential for mammalian skeletal myogenesis. However,the roles of each gene in myogenesis remain unclear, owing partly to genetic linkage at the Myf5/Mrf4 locus...

...Nicole Gensch; Thilo Borchardt; Andre Schneider; Dieter Riethmacher; Thomas Braun The development of myogenic cells is mainly determined by expression of two myogenic factors, Myf5 and Myod1 (MyoD), which genetically compensate for each other during embryogenesis. Here, we demonstrate...

... Mandibular arch Jaw muscles Lbx1 Paraxis En2 Myf5 MyoR Fgf8 Bmp7 Vertebrate skeletal muscles are the basis for all voluntary movements. However, while trunk muscles predominantly serve locomotion and the maintenance of body posture, this is not the purpose of muscles in the head...

... laminin matrix occurs as epaxial myogenic precursor cells enter the myotome. Most Myf5-positive and myogenin-negative myogenic precursor cells localise near assembled laminin, while myogenin-expressing cells are located either away from this matrix or in areas where it is being assembled. In Myf5 nlacZ...

... Epithelial to mesenchymal transition Muscle Myod Myf5 Pax3 Pax7 Somite The embryonic myotome is the source of all epaxial and hypaxial skeletal muscles. The mechanisms underlying myotome development were recently reevaluated ( Brent and Tabin,2002 ; Buckingham,2001 ; Hollway...

... before the eventual onset of terminal differentiation. * Author for correspondence (e-mail: [email protected] ) 25 10 2005 © 2006. 2006 Hypaxial Rectus abdominus Rectus cervicus Geniohyoideus lbx1 myoD myf5 Xenopus laevis Cell...

... development in mouse mutants with genetically disrupted muscle or cartilage development. Through analysis of embryos carrying null mutations in Myf5 and Myod1 , hence lacking both muscle progenitors and differentiated muscle, we identify an essential role for the specified myotome in axial tendon development...

... in the Myf5 epaxial somite (ES)enhancer, is required for the specification of epaxial muscle progenitor cells. Shh signalling is also required for the normal mediolateral patterning of myogenic cells within the somite. In this study, we investigate the role and the transcriptional activities of Gli proteins...

... and differentiation of graft-derived cells were assayed using QCPN and QH1 antibodies to identify all quail cells and quail endothelial cells, respectively. Chimeric embryos were assayed for expression of myf5, myod, paraxis and lbx1 , and the synthesis of myosin heavy chain (MyHC), between 1 and 6 days later (stages...

... and its downstream signal response genes. Development 125 , 777 -790. Borycki, A. G., Brunk, B., Tajbakhsh, S., Buckingham, M.,Chiang, C. and Emerson, C. P., Jr ( 1999 ). Sonic hedgehog controls epaxial muscle determination through Myf5 activation. Development 126 , 4053 -4063. Borycki...

...Marie-Claire Delfini; Delphine Duprez Forced expression of the bHLH myogenic factors, Myf5 and MyoD, in various mammalian cell lines induces the full program of myogenic differentiation. However, this property has not been extensively explored in vivo. We have taken advantage of the chick model...

... this discrepancy, we have re-addressed this issue, using short-term in vivo microsurgery and culture experiments in chick. Our results show that the initial expression of the muscle-specific markers Myf5 and MyoD is regulated in a mesoderm-autonomous fashion. The reception of a Wnt signal is required for MyoD...

...Juliette Hadchouel; Jaime J. Carvajal; Philippe Daubas; Lola Bajard; Ted Chang; Didier Rocancourt; David Cox; Dennis Summerbell; Shahragim Tajbakhsh; Peter W. J. Rigby; Margaret Buckingham Myf5 is the first myogenic regulatory factor to be expressed in the mouse embryo and it determines the entry...