... (SCs) and in skeletal myotubes. Here, we examined the role of Runx1 in mouse satellite cells to determine the role of Runx1 during muscle differentiation. Conditional deletion of Runx1 in adult SCs negatively impacted self-renewal and impaired skeletal muscle maintenance even though Runx2 expression...

...Briana Christophers; Shannon N. Leahy; David B. Soffar; Victoria E. von Saucken; Kendal Broadie; Mary K. Baylies ABSTRACT Cofilin, an actin-severing protein, plays key roles in muscle sarcomere addition and maintenance. Our previous work found that Drosophila cofilin ( DmCFL ) knockdown in muscle...

... repair by scar formation and the initiation of tissue regeneration. They initially assume inflammatory phenotypes, releasing pro-inflammatory cytokines and removing dead and dying tissue, before entering a reparative stage, replacing dead muscle tissue with a non-contractile scar. In this Review, we...

...Brittany C. Collins; Gabrielle Kardon ABSTRACT Vertebrate skeletal muscle is composed of multinucleate myofibers that are surrounded by muscle connective tissue. Following injury, muscle is able to robustly regenerate because of tissue-resident muscle stem cells, called satellite cells. In addition...

... in Drosophila , however, have begun to reveal new roles of nuclear receptor signaling in peripheral tissues in controlling reproduction. Here, we identified Hormone receptor 4 (Hr4) as an oogenesis regulator required in the ovary and muscles. Global Hr4 knockdown leads to increased germline stem cell (GSC) loss...

...Myung-Jun Kim; Michael B. O'Connor ABSTRACT The Myostatin/Activin branch of the TGF-β superfamily acts as a negative regulator of vertebrate skeletal muscle size, in part, through downregulation of insulin/insulin-like growth factor 1 (IGF-1) signaling. Surprisingly, recent studies in Drosophila...

... Nuclear lamina Muscle Nuclear envelope Endoreplication emerges as an important strategy of differentiated cells, enabling them to grow in size or rescue tissue integrity following injury, in a wide range of non-dividing cell types ( Gan et al., 2019 ). Recent experimental studies have proposed...

...Daniel P. S. Osborn; Kuoyu Li; Stephen J. Cutty; Andrew C. Nelson; Fiona C. Wardle; Yaniv Hinits; Simon M. Hughes ABSTRACT Skeletal muscle derives from dorsal mesoderm formed during vertebrate gastrulation. Fibroblast growth factor (Fgf) signalling cooperates with Tbx transcription factors...

...Deli Yu; Izumi Oda-Ishii; Atsushi Kubo; Yutaka Satou ABSTRACT Striated muscle cells in the tail of ascidian tadpole larvae differentiate cell-autonomously. Although several key regulatory factors have been identified, the genetic regulatory pathway is not fully understood; comprehensive...

..., and caused peripheral nervous system defects. Laminin Collagen Perlecan Extracellular matrix ECM Muscle Dorsal median cells Axon guidance Morphogenesis NMJ Proteome analysis of isolated basement membranes (BMs) has identified over 100 associated proteins, highlighting the complex...

... that appears to be essential for efficient inter-membranous exchange of RNA, proteins and ions. We found that the membrane-associated Muscle-specific α2/δ (Ma2/d) subunit of the Ca 2+ channel complex localizes to the SR and T-tubules, and accumulates at the myonuclear surfaces. Furthermore, Ma2/d mutant larval...

... Organogenesis Cell-cell fusion Syncytin Fusexins EFF-1 HAP2 GCS1 AFF-1 Myomaker Myomixer Myomerger Minion Placenta Muscle Virus-cell fusion Tick-borne encephalitis Zika Influenza Dengue viruses SNAREs Hemifusion Pore formation Israel Science Foundation 10.13039/501100003977 443...

...Manuela Weitkunat; Martina Brasse; Andreas R. Bausch; Frank Schnorrer Muscle forces are produced by repeated stereotypical actomyosin units called sarcomeres. Sarcomeres are chained into linear myofibrils spanning the entire muscle fiber. In mammalian body muscles, myofibrils are aligned laterally...

...Peter C. D. Macpherson; Pershang Farshi; Daniel Goldman Muscle denervation resulting from injury, disease or aging results in impaired motor function. Restoring neuromuscular communication requires axonal regrowth and endplate reinnervation. Muscle activity inhibits the reinnervation of denervated...

...Elly Ordan; Talila Volk Coordinated locomotion of an organism relies on the development of proper musculoskeletal connections. In Drosophila , the Slit-Robo signaling pathway guides muscles to tendons. Here, we show that the Slit receptor Roundabout 2 (Robo2) plays a non-cell-autonomous role...

...Elly Ordan; Marko Brankatschk; Barry Dickson; Frank Schnorrer; Talila Volk During organogenesis, secreted signaling proteins direct cell migration towards their target tissue. In Drosophila embryos, developing muscles are guided by signals produced by tendons to promote the proper attachment...

...Liora Kutchuk; Anu Laitala; Sharon Soueid-Bomgarten; Pessia Shentzer; Ann-Helen Rosendahl; Shelly Eilot; Moran Grossman; Irit Sagi; Raija Sormunen; Johanna Myllyharju; Joni M. Mäki; Peleg Hasson Muscle is an integrated tissue composed of distinct cell types and extracellular matrix. While much...

... expressed in larval body wall muscles and accumulates at the level of Z-bands and around myonuclei. CryAB features a conserved actin-binding domain and, when attenuated, leads to clustering of myonuclei and an altered pattern of sarcomeric actin and the Z-band-associated actin crosslinker Cheerio (filamin...

... to the embryo proper, and are subsequently found within the mesenchyme. A subpopulation of these cells, distinct from embryonic macrophages, co-expresses mesenchymal and hematopoietic markers. In addition, hemogenic endothelium-derived cells contribute to skeletal and smooth muscle, and to other mesodermal...

... dynamics in tissue-specific stem cells during conditions of increased tissue turnover remains limited. Using a TetO-H2B-GFP reporter of proliferative history, we identify skeletal muscle stem cell, or satellite cells, that retain (LRC) or lose (nonLRC) the H2B-GFP label. We show in mice that LRCs...