... CPE. * Authors for correspondence ( [email protected] ; [email protected] ) MRNA deadenylation CCR4–NOT complex Mouse oocyte Translation References Baer , B. W. and Kornberg , R. D. ( 1983 ). The protein responsible for the repeating...

...-mediated forces to the spindle, opening new perspectives regarding this mechanism in mouse oocytes, which differ from most mitotic cells. * Authors for correspondence ( [email protected] ; [email protected] ) Handling Editor: Haruhiko Koseki...

...-culturing with wild-type oocytes. Together, these results indicate that mouse oocytes are deficient in synthesizing cholesterol and require cumulus cells to provide products of the cholesterol biosynthetic pathway. Therefore, oocyte-derived paracrine factors,particularly, BMP15 and GDF9, promote cholesterol...

...Petr Svoboda; Paula Stein; Harutoshi Hayashi; Richard M. Schultz ABSTRACT Specific mRNA degradation mediated by double-stranded RNA (dsRNA), which is termed RNA interference (RNAi), is a useful tool with which to study gene function in several systems. We report here that in mouse oocytes, RNAi...

... function/activity ( Sheets et al., 1994 ) and may share a common regulatory mechanism as for c-mos. The small delay in MAP kinase activation that we observed in KE oocytes may suggest that the synthesis of c-mos, which controls MAP kinase activation in the mouse oocyte ( Verlhac et al., 1996 ), is also...

...Keith T. Jones; John Carroll; Julie A. Merriman; David G. Whittingham; Tomohiro Kono ABSTRACT Mature mouse oocytes are arrested at metaphase of the second meiotic division. Completion of meiosis and a block to polyspermy is caused by a series of repetitive Ca 2+ tran-sients triggered by the sperm...

...Jacek Kubiak; Andras Paldi; Michèle Weber; Bernard Maro ABSTRACT The microfilament inhibitor cytochalasin D inhibits extrusion of the first polar body when present during the first meiotic division of mouse oocytes; however, it does not interfere with anaphase movement of chromosomes, and thus...

... by growing mouse oocytes and accumulate to unusually high levels in fully grown oocytes as compared to somatic cells; 240000, 200000 and 74000 copies mRNA per fully grown oocyte for ZP3, LDH-B and MOM-1, respectively. Steady-state levels of LDH-B and MOM-1 mRNA undergo a modest decline (∼20–40 %) during...

...H. J. Clarke; J. Rossant; Y. Masui ABSTRACT Mouse oocytes at metaphase I were treated with puromycin, which caused the chromosomes to become decondensed within an interphase nucleus. When the oocytes were allowed to resume protein synthesis, they returned to metaphase within 8 –10 h and neither...

...Jonathan Van Blerkom; Hobart Bell ABSTRACT The relationship between nuclear maturation and the differentiation of the cytoplasm and plasma membrane during resumption of arrested meiosis was investigated by culture of GV- and Mil-stage mouse oocytes in the presence and absence of nocodazole. Culture...

...Anna Niemierko; Aldona Komar ABSTRACT Phorbol ester (PMA) in concentration 5 and 10 ng ml -1 blocks cytokinesis of the second maturation division in mouse oocytes. Karyokinesis is not impaired and digynic triploid oocytes are obtained which undergo first cleavage division. Effectiveness of blocking...