...) specification are incompletely understood owing, in part, to limitations in distinguishing CPCs from non-cardiac mesoderm in early gastrulation. We leveraged detection of early cardiac lineage transgenes within a granular single-cell transcriptomic time course of mouse embryos to identify emerging CPCs...

... tissue interactions at key time points in the mouse embryo. We demonstrate the utility of the approach by providing volumetric data, 3D distribution of three main cellular components (epithelial, mesenchymal and endothelial cells) within the developing pancreas, and quantification of their relative...

...Simone Probst; Sagar; Jelena Tosic; Carsten Schwan; Dominic Grün; Sebastian J. Arnold ABSTRACT Anterior mesoderm (AM) and definitive endoderm (DE) progenitors represent the earliest embryonic cell types that are specified during germ layer formation at the primitive streak (PS) of the mouse embryo...

... of the organelle. Although mutations in MRPs have been associated with human diseases, little is known about their role during development. Presented here are the null phenotypes for 21 nuclear-encoded mitochondrial proteins and in-depth characterization of mouse embryos mutant for the Mrp genes Mrpl3 , Mrpl22...

... molecular markers to identify them. Here, we elucidate the expression pattern of the transcription factor Nkx1-2 in the mouse embryo and show that it identifies axial progenitors throughout body axis elongation, including neuromesodermal progenitors and early neural and mesodermal progenitors. We create...

... transcription in early mouse embryos and embryonic stem cells via antagonism of histone H3 lysine 9/14 acetylation . Mol. Cell. Biol.   35 , 4158 - 4169 . 10.1128/MCB.00546-15 Carrero , G. , McDonald , D. , Crawford , E. , de Vries , G. and Hendzel , M. J. ( 2003 ). Using FRAP...

... compromises migration of myogenic cells to the limb. Mouse embryo Forelimb bud Myogenic cells Somite-derived endothelial cells Pax3 Foxc1 Foxc2 Foxc1 and Foxc2 transcription factors are important for tissue and organ development at different sites in the mouse embryo ( Kume, 2009 , 2010...

... approaches to constitutively activate the NOTCH pathway in the mouse embryo. Early embryonic development, including the establishment of anterior-posterior polarity, is not perturbed by forced NOTCH activation. By contrast, widespread NOTCH activity in the epiblast triggers dramatic gastrulation defects...

... organisms such as early post-implantation mouse embryos. We introduce here a microdevice to achieve localised electroporation with high efficiency and reduced cell damage. In silico simulations using a simple electrical model of mouse embryos indicated that a dielectric guide-based design would improve...

.... A. , Gutierrez-Adan A. ( 2012 ). Long-term and transgenerational effects of in vitro culture on mouse embryos . Theriogenology 77 , 785 – 793 . Cantone I. , Fisher A. G. ( 2013 ). Epigenetic programming and reprogramming during development . Nat. Struct. Mol. Biol. 20 , 282 – 289...

... The authors declare no competing financial interests. 30 1 2013 © 2013. Published by The Company of Biologists Ltd 2013 Axon development Cytoskeleton Motoneurons Mouse embryo Neuromuscular junction Clip3 Cytoplasmic linker proteins, or CLIPs, define a family...

... specification of embryonic blastomeres by sequentially separating the blastomeres in two-cell stage mouse embryos and continuing separation after each cell division throughout pre-implantation development. This procedure eliminates information provided by cell-cell interaction and cell positioning. Gene...

... of the generated mice will be embryonic lethal; therefore, phenotyping methods tuned to the mouse embryo are needed. Methods that are robust, quantitative, automated and high-throughput are attractive owing to the numbers of mice involved. Three-dimensional (3D) imaging is a useful method for characterizing...

... financial interests. 28 5 2012 © 2012. Pluripotent stem cells Directed differentiation Extra-embryonic endoderm FGF Mouse embryo Early mammalian embryogenesis is characterized by a gradual restriction in the developmental potential of cells that constitute...

... to the gastrulating mouse conceptus suggests manifold roles in mammalian development . Dev. Dyn. 237 , 464 – 475 . Downs K. M. , Davies T. ( 1993 ). Staging of gastrulating mouse embryos by morphological landmarks in the dissecting microscope . Development 118 , 1255 – 1266 . Forlani...

.... Dermis Mouse embryo Fibroblasts Hair follicle Wnt β-catenin The dermis contributes to the structural integrity and function of skin. Dermal fibroblasts in the dermis are crucial for hair follicle formation and wound healing ( Millar, 2002 ). Dermal fibroblasts orchestrate...

...Marieke Cajal; Kirstie A. Lawson; Bill Hill; Anne Moreau; Jianguo Rao; Allyson Ross; Jérôme Collignon; Anne Camus In the mouse embryo the anterior ectoderm undergoes extensive growth and morphogenesis to form the forebrain and cephalic non-neural ectoderm. We traced descendants of single ectoderm...

...Daniel W. Stuckey; Melanie Clements; Aida Di-Gregorio; Claire E. Senner; Paul Le Tissier; Shankar Srinivas; Tristan A. Rodriguez During development, the growth of the embryo must be coupled to its patterning to ensure correct and timely morphogenesis. In the mouse embryo, migration of the anterior...

[email protected] ) Competing interests statement The authors declare no competing financial interests. 17 12 2010 © 2011. 2011 Mouse embryo Wilms' tumour suppressor gene Retinoic acid Raldh2 Epicardium Retinoic acid (RA) regulates a variety of crucial events during early...