...Rocco J. Rotello; Pierre-Alain Fernandez; Junying Yuan ABSTRACT We have isolated a group of monoclonal antibodies that specifically recognize either apoptotic or engulfment cells in the interdigit areas of chicken hind limb foot plates, and throughout the embryo. Ten of these antibodies (anti...

...Shohei Mjtani; Harumasa Okamoto ABSTRACT Neural induction of ectoderm cells has been reconstituted and examined in a microculture system derived from dissociated early gastrula cells of Xenopus laevis . We have used monoclonal antibodies as specific markers to monitor cellular differentiation from...

.... * Author for correspondence 04 12 1989 14 05 1990 © 1990 by Company of Biologists 1990 cortical differentiation monoclonal antibodies protein distribution Tetrahymena membrane skeleton basal body There is growing interest in the problem of cortical organization...

... of monoclonal antibodies (Mabs) specific for the C μ and C v heavy Ig chain isotypes and for the light chain constituents shared by IgM and IgY molecules. Clusters of IgM- and of IgY-synthesizing lymphocytes, often located in separate sites, are first present in spleen sections of 7-week-old 25 mm larvae, about...

...Ian E. Crandall; Peter C. Newell ABSTRACT We have produced a series of monoclonal antibodies that recognize carbohydrate epitopes on cell surface glycoproteins of developing amoebae of Dictyostelium discoideum . The antibodies were found to have differential specificity for amoebae at different...

... developmental stage. P-cadherin, however, gradually disappears during development, initially from the main bronchi and eventually from all epithelial cells. When a monoclonal antibody to E-cadherin (ECCD-1) was added to monolayer cultures of lung epithelial cells, it induced a partial disruption of their cell...

... of the upper lip skin in vitro in the absence or presence of a monoclonal antibody to E-cadherin (ECCD-1) or to P-cadherin (PCD-1). In control cultures, skin morphogenesis normally occurred in a pattern whereby the hair follicles grew and dermal cells were condensed to form the dermal sheath. A mixture of ECCD...

.... In an immunohistochemical study using collagen type-specific monoclonal antibodies, we asked whether type IX collagen, another ‘cartilage-specific’ collagen, is coexpressed along with type II at such interfaces. We confirmed that, in the matrix underlying a variety of cranial ectodermal derivatives and along the ventro...

...Keiji Itoh; Akiko Yamashita; Hiroshi Y. Kubota ABSTRACT Five kinds of monoclonal antibodies that are specific for the epidermis of Xenopus embryos were produced. Epidermis-specific antibodies were used to investigate the spatial and temporal expressions of epidermal antigens during embryonic...

...Michel Piovant; Philippe Léna We have generated a monoclonal antibody (Mab E1C) that recognizes the differentiated nervous system in Drosophila embryos. At the cellular blastoderm stage, Mab E1C behaves as a general ectodermal marker but, in subsequent stages, it also labels the mesoderm...

...Robert A. McCarthy; Max M. Burger ABSTRACT Laminin, a component of the embryonic sea urchin basal lamina, is recognized by monoclonal antibody BL1 (Mab BL1). Our results demonstrate that laminin is secreted into the blastcoel at the early blastula stage at a time when the blastomeres undergo a cell...

...C. Abbadie; D. Boucher; J. Charlemagne; J. C. Lacroix ABSTRACT The location of three proteins of the oocyte nucleus of Pleurodeles was studied during oogenesis and embryogenesis using monoclonal antibodies A33/22, C3/1 and C36/1. Immunoblotting of two-dimensional gel electophoregrams of oocyte...

... in the wild-type strain WS380B; it is observed shortly after the advancing tip contacts the substratum. Stationary collars formed approximately every 12 min; they were matched with patterns revealed on the underside of slime trails with FITC-coupled monoclonal antibody MUD50. It is proposed that stationary...

.... Monoclonal antibodies were prepared against the isolated cytoplasm. Twelve monoclonal antibodies, identified by indirect immunofluorescence, stained the myoplasmic region. When unfertilized eggs were centrifuged, stratifying their mitochondria and some other cytoplasmic components, components identified...

...Izumi Mita-Miyazawa; Takahito Nishikata; Noriyuki Satoh ABSTRACT To obtain specific immunological probes for studying molecular mechanisms involved in the early embryonic development of ascidians, we have produced monoclonal antibodies directed against a homogenate of larvae of the ascidian...

... yolk platelet compartments monoclonal antibodies cytoskeleton proteins. The animal/vegetal (A/V) polarity of the typical amphibian egg is immediately recognizable by the distinct pigmentation differences between the darkly pigmented (animal) and lightly pigmented (vegetal) hemispheres...

...S. F. Godsave; B. H. Anderton; C. C. Wylie ABSTRACT Antibodies against various intermediate filament proteins have been used to follow cell differentiation in the early Xenopus embryo. Three new monoclonal antibodies against Xenopus cytokeratins raised against Triton-insoluble material from...

...Marian G. Langer; C. V. Sundarraj; Nirmala Sundarraj ABSTRACT Monoclonal antibodies, specific against cell surface differentiation antigens of human corneal epithelial cells, were developed using epithelial cells resected from human corneas as the immunogens. One of these antibodies reacted...

..., the molecule is present at a concentration of 2·5×10 3 molecules μ m -2 , which approximates HLA/ABC concentration on lymphocytes. By comparing the reactivity of four anti-XT-1 monoclonal antibodies, three of which elicit germ cell-germ cell adhesion, we have defined two distinct surface regions of the XT-l...

...Wai Chang Ho; Kathleen B. Bechtol ABSTRACT Four monoclonal antibodies, XT-I, MT-23, MT-24 and MT-29, that bind the XT-1-differentiation-antigen of male germ cells have been used to investigate the biological role of the XT-1-molecule of germ cells in short-term primary culture. Cultures from 10...