... link between Ednrb signaling and the DNA sensor/tumor suppressor Aim2 in regulating neural crest-derived melanoblast proliferation. Ednrb signaling MITF Melanocytes White spotting Pigmentation National Natural Science Foundation of China http://dx.doi.org/10.13039/501100001809...

.... Summary: This Review summarises the latest advances in understanding how pigment-producing melanocytes develop from the neural crest, Schwann cell progenitors and melanocyte stem cells, and how these mechanisms are co-opted in melanoma. Melanocytes Melanocyte stem cells Neural crest MITF...

... the melanocyte lineage. Indeed, β-catenin regulates transcription of Mitf-M, the master regulator of this lineage. The first wave of melanocytes to colonize the skin is directly derived from neural crest cells, whereas the second wave of melanocytes is derived from Schwann cell precursors (SCPs). We investigated...

... through which vasculature enters the eye and ganglion cell axons exit. Although many factors have been identified that function during CF closure, the molecular and cellular mechanisms mediating this process remain poorly understood. Failure of CF closure results in colobomas. Recently, MITF was shown...

... and differentiation, melanocyte stem cells, and the role of the melanocyte lineage in diseases such as melanoma. MITF Melanoma Neural crest Stem cells Melanin, the pigment that colours the skin, eyes, hair, fur, feathers and scales of vertebrates (see Box 1 ), is made by specialised cells called...

... or inhibition of Wnt, but not Wnt/β-catenin, signalling prevents pigmentation and downregulates the RPE regulatory gene Mitf . Activation of BMP or Wnt signalling can replace the surface ectoderm to rescue MITF expression and optic cup formation. We provide evidence that BMPs and Wnts cooperate via a GSK3β...

..., ocular coloboma. Defective FGF signaling does not abrogate Shh or BMP signaling, nor does it affect axial patterning of the optic vesicle. Instead, FGF signaling regulates Mitf and Pax2 in coordinating the closure of the optic fissure and optic disc specification, which is necessary for the outgrowth...

... the melanogenic markers Mitf and MC/1 and the guidance receptor Ednrb2, generating instead glial cells that express P0 and Fabp. In this context, Foxd3 lies downstream of Snail2 and Sox9 , constituting a minimal network upstream of Mitf and Ednrb2 to link melanogenic specification with migration. Consistent...

.... Mitf Dct β-Catenin Melanoblast Mouse References Adameyko I. , Lallemend F. , Aquino J. B. , Pereira J. A. , Topilko P. , Muller T. , Fritz N. , Beljajeva A. , Mochii M. , Liste I. , et al . ( 2009 ). Schwann cell precursors...

... is expressed exclusively in the neural/glial precursors and MITF is expressed only in melanoblasts. Moreover, FOXD3 represses melanogenesis. Here we show that avian MITF expression begins very early during melanoblast migration and that loss of MITF in melanoblasts causes them to transdifferentiate to a glial...

... (RPE) progenitors expressing the transcription factor Mitf. Chx10 mutations lead to microphthalmia and impaired neuroretinal proliferation. Mitf mutants have a dorsal RPE-to-neuroretinal phenotypic transformation, indicating that Mitf is a determinant of RPE identity. We report here that Mitf...

.... This program involved the expansion of expression of genes normally found only in the periphery into central regions of the eye. These genes included a transcription factor controlling pigmentation, Mitf , and the related factor Tfec ( Tcfec – Mouse Genome Informatics), which can activate a melanogenic gene...

... that in contrast to Kitl -positive Mitf mi–ew /Mitf mi–ew NTs, Kitl -deficient NTs do not allow for Ednrb -deficient cells to become tyrosinase positive ( Fig. 3C ). As there is no reason to assume that the conditions for potential cell fusion in these co-cultures was any different from those in co...

... specific isoform of the microphtalmia-related transcription factor (Mitf). Mitf is a basic helix-loop-helix-leucine zipper transcription factor whose M isoform is restricted to neural crest derived melanocytes and is essential for the development of these cells in vertebrates from mammals to fish. What...

...-epithelial and optic-stalk progenitor domains, suggesting redundant functions during retinal determination. Pax2; Pax6 compound mutants display a dose-dependent reduction in the expression of the melanocyte determinant Mitf, accompanied by transdifferentiation of retinal pigmented epithelium (RPE...

... of the transcription factor mitfa , which itself is required for melanophore development. We demonstrate that the zebrafish mitfa promoter has sox10 binding sites necessary for activity in vitro, consistent with studies using mammalian cell cultures that have shown that Sox10 directly regulates Mitf expression...

... is required for the induction and maintenance of expression of the RPE-specific genes Mitf and Wnt13 and the melanosomal matrix protein MMP115. In the absence of extraocular tissues, RPE development did not occur. Replacement of the extraocular mesenchyme with cranial mesenchyme, but not lateral plate...

... or no role in wild-type fin stripe development, thus revealing a secondary mode for regulation of fin stripes. Expression of melanocyte markers in regenerating kit mutant fins suggests that kit normally acts after mitf and before dct to promote development of the primary kit -dependent melanocytes. kit...

... in melanophores. We show that nac w2 is a mutation in a zebrafish gene encoding a basic helix-loop-helix/leucine zipper transcription factor related to microphthalmia ( Mitf ), a gene known to be required for development of eye and crest pigment cells in the mouse. Transient expression of the wild-type nacre gene...

... and developed with NBT/BCIP. The Microphthalmia ( mitf ) gene product is a transcription factor known to be essential for melanocyte differentiation. At the wing level of stage 19 avian embryos, cells expressing mitf mRNA were first detected in the crest cell population that remained in the MSA...