Fig. 1. Epigenomic analysis of H. miamia development identifies regions of accessible chromatin. (A) Schematic phylogenetic tree of selected metazoan lineages demonstrating the placement of Xenacoelomorpha (pink outline) as the likely sister group to all other bilaterians or sister to the Ambu... More about this image found in Epigenomic analysis of H. miamia development identifies regions of access...

Fig. 2. Differential accessibility across the H. miamia genome reflects major developmental transitions. (A) Heatmap of all chromatin peaks that show significant changes during H. miamia development. (B) Fuzzy c-means clustering of regions of accessible chromatin with each cluster plotted in... More about this image found in Differential accessibility across the H. miamia genome reflects major dev...

Fig. 3. Genome-wide analyses implicate key transcription factor motifs in developmental lineage decision-making. (A) t-Distributed stochastic neighbor embedding (t-SNE) plot showing groupings of samples, including biological replicates, based on chromatin accessibility of the consensus peakset. ... More about this image found in Genome-wide analyses implicate key transcription factor motifs in developme...

Fig. 4. RUNT is among the most variably accessible motifs across H. miamia development, suggesting Runt as a major regulator of early development . (A) URD tree with major tissue lineages labeled ( Kimura et al., 2022 ). (B) URD tree with expression of runt . The color of the branch represents... More about this image found in RUNT is among the most variably accessible motifs across H. miamia develo...

Fig. 5. Motif enrichment and predicted binding provide hypotheses for regulatory relationships of known epidermal differentiation genes. (A) Accessibility of the p63 / p73 gene locus with candidate RUNT-binding sites. (B) URD tree showing p63 / p73 expression, with the epidermal lineage outl... More about this image found in Motif enrichment and predicted binding provide hypotheses for regulatory re...

Fig. 6. Predicted transcription factor binding uncovers a regulatory network with Pitx, Fox and Zic transcription factors in H. miamia muscle development. (A) URD tree with muscle lineages outlined. (B) tropomyosin gene expression (magenta), and tropomyosin antibody staining (green) in devel... More about this image found in Predicted transcription factor binding uncovers a regulatory network with P...

Fig. 1. FD and FDP are expressed in floral organs. (A) In situ hybridization of (i) FD and (ii) FDP mRNAs in inflorescences of plants grown for 20 long days (LDs) (i) and in 2-week-old plants grown under short days and then transferred to LDs for 9 days (ii). (B) Confocal images of (i)... More about this image found in FD and FDP are expressed in floral organs. (A) In situ hybridization ...

Fig. 2. The fd fdp double mutant shows floral organ defects under long-day conditions. (A) Representative flowers of the genotypes illustrated. The red arrowheads indicate petals and the orange arrowhead indicates sepals. (B) The numbers of sepals, petals and stamens in flowers of the illustra... More about this image found in The fd fdp double mutant shows floral organ defects under long-day condit...

Fig. 3. The ft tsf double mutant shows floral defects under long-day conditions. (A) Floral phenotypes of an inflorescence/flower 30-day-old Col-0 (i,ii) and 45-day-old ft-10 tsf-1 (iii,iv) plants, showing petal loss (red arrowheads). Carpels in ft-10 (stage 15 and 16 siliques) resemble Co... More about this image found in The ft tsf double mutant shows floral defects under long-day conditions. ...

Fig. 4. Floral defects in ft-10 tsf-1 under short-day conditions. (A) Flowering time under short-day conditions (SDs) of the Col-0 and ft-10 tsf-1 plants shown as rosette (left) and cauline leaf numbers (right). Asterisks indicate significant differences (unpaired Student's t -test: **** P ... More about this image found in Floral defects in ft-10 tsf-1 under short-day conditions. (A) Flowering ...

Fig. 5. ft tsf and fd fdp double mutants show defects in floral meristem size and shape. (A) 3D confocal images of stage 4 floral buds: Col-0 (i), fd-3 fdp-CRP2 (ii) and ft-10 tsf-1 (iii). (B) Quantified floral meristem width ( n : Col-0=17; ft-10 tsf-1 =16; fd-3 fdp-CRP2 =24) with sig... More about this image found in ft tsf and fd fdp double mutants show defects in floral meristem size an...

Fig. 6. Presence of FT:GFP in floral meristems and complementation of floral organ defects. (A) Confocal images of a SUC2::FT:GFP ft-7 25-day-old plant (i,ii) and a pFT::FT:GFP ft-7 30-day-old plant at various stages (iii-v), showing FT:GFP signal (white arrowheads) in the vasculature (i,ii)... More about this image found in Presence of FT:GFP in floral meristems and complementation of floral organ ...

Fig. 7. FT TSF and FD FDP positively activate SEP genes and AG , and repress WUS in developing flowers. (A) In situ hybridization of AG mRNA in inflorescences of the depicted genotypes. Flower buds shown were at the indicated stages. Plants were grown for 24 LDs for Col-0, for 28 LDs for ... More about this image found in FT TSF and FD FDP positively activate SEP genes and AG , and repress WUS ...

Fig. 8. Comparison of FD ChIPseq gene targets with the targets of other floral organ identity MADS-box transcription factors. (A) Binding profiles of FD and MADS box transcription factors to SEP1 , SEP2 and SEP3 , and other genes involved in flower development. The panels display, from the t... More about this image found in Comparison of FD ChIPseq gene targets with the targets of other floral orga...