...Chisato Kunitomi; Mayra Romero; Enrico Maria Daldello; Karen Schindler; Marco Conti ABSTRACT The RNA-binding protein cytoplasmic polyadenylation element binding 1 (CPEB1) plays a fundamental role in regulating mRNA translation in oocytes. However, the specifics of how and which protein kinase...

... that nuclear-associated P granules provide a perinuclear compartment where newly exported mRNAs are collected prior to their release to the general cytoplasm. First, we show that mRNA export factors are highly enriched at the NPCs associated with P granules. Second, we discovered that the expression of high...

... systems and transcription factors not previously reported in early sea urchin embryos, including components of Notch and TGF signal transduction pathways. As expected from earlier kinetic analyses of the embryo mRNA populations, no very prevalent protein-coding species were encountered; the most highly...

...Geraldine Seydoux; Melanie A. Dunn ABSTRACT Early embryonic germ cells in C. elegans and D. melanogaster fail to express many messenger RNAs expressed in somatic cells. In contrast, we find that ribosomal RNAs are expressed in both cell types. We show that this deficiency in mRNA production...

...Sean Wyatt; Alun M. Davies ABSTRACT We have used a quantitative reverse transcription/polymerase chain reaction amplification technique to study the regulation of p75 mRNA and trkA mRNA expression in developing NGF-dependent trigeminal neurons. Before becoming NGF dependent, these neurons express...

... blots, we find evidence for two Psc mRNAs and, on western blots, we find evidence for two Psc proteins that are altered either in abundance or size in Psc mutants. The Psc protein accumulates in all regions of the embryo and also in many tissues in a variety of developmental stages. In all cases...

...Laura L. Harris; John C. Talian; Peggy S. Zelenka ABSTRACT The present study uses the polymerase chain reaction and in situ hybridization to examine c- myc and N- myc mRNA in the embryonic chicken lens at 6,10,14 and 19 days of development and compares the pattern of expression obtained...

... to be proximal. At birth, practically all proximal tubules expressed the two antigens. This analysis of GJ proteins was consistent with the results of S1 nuclease protection assays showing that, while the α 1 mRNA appeared early during kidney development and declined around birth, the two β mRNAs appeared later...

... a soluble 90 ×10 3 M r N-terminal fragment of N-cadherin as well as a number of secreted proteases. Both of these components are also shown to be present in vivo in the vitreous humor. Northern blot analysis indicates a single mRNA encoding N-cadherin in the retina and no evidence for a second message...

... levels of IGF-I mRNA were found in the olfactory bulb and in discrete neurons of the cranial sensory ganglia, notably in the trigeminal ganglion, as early as 13 days of gestation, in the pineal primordium of 18 day old fetuses, and in discrete groups of cells in the cochlear epithelium located laterally...

... and immunofluorescence histochemistry on selected stages of embryonic chick tibiotarsi. The data show that mRNA for type X collagen appears at or about the time that we detect the first appearance of the protein. This result is incompatible with translational regulation, which would require accumulation of the mRNA...

... lacking the A chain. We have here studied the occurrence of the isoforms during mouse organogenesis. In all tissues studied, the A chain mRNA and polypeptide were more weakly expressed than those of the B chains. Laminin A chain polypeptides showed a much more restricted tissue distribution than the B...

... rapidly in stage IV oocytes than in previtellogenic stage I+II oocytes. Quantitative Northern analysis showed that ornithine decarboxylase mRNA is abundant in oocytes (about 8×l0 8 transcripts/cell) and this number does not significantly change during oogenesis. Polysome analysis showed that this mRNA...

... expression during spermatogenesis was examined by Northern blot analysis using specific cDNA probes. Total RNA was isolated from adult tissues, enriched germinal cell populations and polysomal gradients of total testis and isolated germinal cells. Three cytochrome C S mRNAs were detected averaging 1.3 kb...

... hybridization experiments with mouse embryos and adult tissues, we first detected C/EBP mRNA in hepatocytes at the 13th gestational day, when no other cell types give detectable signals and thus by this test C/EBP is, at least in the embryo, a ‘liver-specific’ factor. Only trace amounts of C/EBP were seen...

... hybridization (ISH) in the developing rat. Expression occurs in extra-embryonic membranes at the time of IGF-n mRNA induction and later at paracrine/ autocrine sites of IGF-II action (skeletal muscle and perichondrium) in the embryo. Highest levels of receptor mRNA occur in heart and major vessels. Postnatally...

...J. R. Collier ABSTRACT Two-dimensional electrophoresis of labeled proteins and hybridization of mRNAs with specific gene probes was used to demonstrate changes in protein synthesis, and the cytoplasmic control of actin maternal mRNAs during the early development of llyanassa . The isolated polar...

...R. J. Roller; R. A. Kinloch; B. Y. Hiraoka; S. S.-L. Li; P. M. Wassarman ABSTRACT Ribonuclease protection assays have been used to quantitatively assess changes in steady-state levels of specific mRNAs during oogenesis and early embryogenesis in mice. The mRNAs encode ZP3 (a glycoprotein...

...Bernadette Fouquet; Harald Herrmann; Jürgen K. Franz; Werner W. Franke A Xenopus laevis mRNA encoding a cytokeratin of the basic (type II) subfamily that is expressed in postgastrulation embryos was cDNA-cloned and sequenced. Comparison of the deduced amino acid sequence of this polypeptide (513...

... histochemistry) and for Northern blotting. IGF-II mRNA is strongly expressed in liver, skeletal muscle, perichondrium, leptomeninges and choroid plexus of the newborn. Demonstrable levels fall dramatically in the liver at 18-20 days postnatally but persist for longer periods in muscle and remain undiminished...