...Fatima N. Chaudhry; Sylvia N. Michki; Dain L. Shirmer; Sharon McGrath-Morrow; Lisa R. Young; David B. Frank; Jarod A. Zepp ABSTRACT Alveologenesis, the final stage in lung development, substantially remodels the distal lung, expanding the alveolar surface area for efficient gas exchange. Secondary...

.... Importantly, deficits in vascular development lead to significant pediatric lung diseases, indicating a need to uncover fetal programs promoting vascular growth. To address this, we used a transgenic mouse reporter for expression of Cxcl12 , an arterial endothelial hallmark gene, and performed single-cell RNA...

... 9 12 2021 © 2022. Published by The Company of Biologists Ltd 2022 Summary: Wnts from the lung epithelium are crucial for the differentiation of the surrounding mesenchyme during early postnatal alveologenesis. Alveologenesis Bronchopulmonary dysplasia Lung Wnt Wntless...

... for the development of most organs, including the lung. Despite extensive work detailing effects of RA deficiency in early lung morphogenesis, little is known about how RA regulates late gestational lung maturation. Here, we investigate the role of the RA catabolizing protein Cyp26b1 in the lung. Cyp26b1 is highly...

...Thomas Volckaert; Tingting Yuan; Jie Yuan; Eistine Boateng; Seantel Hopkins; Jin-San Zhang; Victor J. Thannickal; Reinhard Fässler; Stijn P. De Langhe ABSTRACT Organ growth and tissue homeostasis rely on the proliferation and differentiation of progenitor cell populations. In the developing lung...

...Leah B. Nantie; Randee E. Young; Wyatt G. Paltzer; Yan Zhang; Randy L. Johnson; Jamie M. Verheyden; Xin Sun ABSTRACT Lung growth to its optimal size at birth is driven by reiterative airway branching followed by differentiation and expansion of alveolar cell types. How this elaborate growth...

... mechanisms of cell fate regulation through niche-derived cues, with a particular focus on epithelial stem cells of the mammalian skin, intestine and lung. We discuss a spectrum of niche-derived biochemical, mechanical and architectural inputs that define stem cell states during morphogenesis, homeostasis...

...Mei-I Chung; Melissa Bujnis; Christina E. Barkauskas; Yoshihiko Kobayashi; Brigid L. M. Hogan ABSTRACT The bone morphogenetic protein (BMP) signaling pathway, including antagonists, functions in lung development and regeneration of tracheal epithelium from basal stem cells. Here, we explore its...

... of pluripotent stem cells (PSCs) through stimulation of developmental signaling pathways can generate mature somatic cell types for basic laboratory studies or regenerative therapies. However, there has been significant uncertainty regarding a method to separately derive lung versus thyroid epithelial lineages...

... role in lung epithelium, consistent with lack of HOXA5 expression in this cell layer. In contrast, ablation of Hoxa5 in mesenchyme perturbed trachea development, lung epithelial cell differentiation and lung growth. Further, deletion of Hoxa5 in motor neurons resulted in abnormal diaphragm innervation...

... signalling is required for self-renewing divisions of basal stem cells and to maintain expression of the key transcription factor SOX2. Cre-Lox Lung Mouse Trachea Progenitor Like human airways, the mouse trachea contains three major epithelial lineages ( Rock et al., 2010 ; Teixeira et al...

...; Saverio Bellusci Lipid-containing alveolar interstitial fibroblasts (lipofibroblasts) are increasingly recognized as an important component of the epithelial stem cell niche in the rodent lung. Although lipofibroblasts were initially believed merely to assist type 2 alveolar epithelial cells in surfactant...

...Melinda E. Snitow; Shanru Li; Michael P. Morley; Komal Rathi; Min Min Lu; Rachel S. Kadzik; Kathleen M. Stewart; Edward E. Morrisey The development of the lung epithelium is regulated in a stepwise fashion to generate numerous differentiated and stem cell lineages in the adult lung. How...

...Michael Herriges; Edward E. Morrisey The respiratory system, which consists of the lungs, trachea and associated vasculature, is essential for terrestrial life. In recent years, extensive progress has been made in defining the temporal progression of lung development, and this has led to exciting...

...Mitsuru Morimoto; Ryuichi Nishinakamura; Yumiko Saga; Raphael Kopan In the developing lung, it is thought that the terminal buds of elongating airways contain a population of multipotent epithelial progenitors. As the bronchial tree extends, descendants of these cells give rise to lineage...

... transcriptional repressors Osr1 and Osr2 are redundantly required for Xenopus lung specification in a molecular pathway linking foregut pattering by FGFs to Wnt-mediated lung specification and RA-regulated lung bud growth. FGF and RA signals are required for robust osr1 and osr2 expression in the foregut endoderm...

...Shanru Li; Yi Wang; Yuzhen Zhang; Min Min Lu; Francesco J. DeMayo; Joseph D. Dekker; Philip W. Tucker; Edward E. Morrisey The molecular pathways regulating cell lineage determination and regeneration in epithelial tissues are poorly understood. The secretory epithelium of the lung is required...

...Yongjun Yin; Fen Wang; David M. Ornitz Fibroblast growth factor (FGF) 9 is a secreted signaling molecule that is expressed in lung mesothelium and epithelium and is required for lung development. Embryos lacking FGF9 show mesenchymal hypoplasia, decreased epithelial branching and, by the end...

.... We show that the miR302/367 cluster is a target of the transcription factor Gata6 in mouse lung endoderm and regulates multiple aspects of early lung endoderm progenitor development. miR302/367 is expressed at early stages of lung development, but its levels decline rapidly as development proceeds...

... in a murine Dachsous homolog, Dchs1 . Analysis of the phenotypes of Dchs1 mutant mice and comparisons with Fat4 mutant mice identify requirements for these genes in multiple organs, including the ear, kidney, skeleton, intestine, heart and lung. Dchs1 and Fat4 single mutants and Dchs1 Fat4 double mutants have...