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Keywords: loxP
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Journal Articles

Ubiquitous transgene expression and Cre-based recombination driven by the ubiquitin promoter in zebrafish

Christian Mosimann, Charles K. Kaufman, Pulin Li, Emily K. Pugach, Owen J. Tamplin, Leonard I. Zon
Journal: Development
Development (2011) 138 (1): 169–177.
https://doi.org/10.1242/dev.059345
Published: 1 January 2011
... transplantation experiments to assess true multilineage potential of engrafted cells. We further generated transgenic zebrafish that express ubiquitous 4-hydroxytamoxifen-controlled Cre recombinase activity from a ubi:cre ERt2 transgene, as well as ubi:loxP-EGFP-loxP-mCherry ( ubi:Switch ) transgenics and show...
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Journal Articles

Independent requirements for Hedgehog signaling by both the anterior heart field and neural crest cells for outflow tract development

Matthew M. Goddeeris, Robert Schwartz, John Klingensmith, Erik N. Meyers
Journal: Development
Development (2007) 134 (8): 1593–1604.
https://doi.org/10.1242/dev.02824
Published: 15 April 2007
... for correspondence (e-mail: [email protected] ) 30 1 2007 © 2007. 2007 Anterior heart field (AHF) Neural crest Shh Outflow tract Congenital heart defect Hedgehog Cre loxP Septation Mouse The morphogenesis of the outflow tract (OFT) of the heart is a complex process...
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Includes: Supplementary data
Journal Articles

Endothelium-specific ablation of PDGFB leads to pericyte loss and glomerular, cardiac and placental abnormalities

Mattias Bjarnegård, Maria Enge, Jenny Norlin, Sigrun Gustafsdottir, Simon Fredriksson, Alexandra Abramsson, Minoru Takemoto, Erika Gustafsson, Reinhard Fässler, Christer Betsholtz
Journal: Development
Development (2004) 131 (8): 1847–1857.
https://doi.org/10.1242/dev.01080
Published: 15 April 2004
... ). Deletion of exon 4 in the Pdgfb gene results in a null allele( Levéen et al., 1994 ). To generate a conditionally inactivated Pdgfb allele, loxP sites were positioned on either side of exon 4 by homologous recombination in ES-cells. This resulted in a functionally intact Pdgfb flox allele( Enge et...
View article titled, Endothelium-specific ablation of PDGFB leads to pericyte loss and glomerular, cardiac and placental abnormalities
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