..., a fusion protein consisting of the plus-end-directed microtubule motor kinesin and β-galactosidase (Kin:βgal) similarly localizes to the posterior pole, thereby suggesting that plus ends of microtubules are pointed to the posterior. In this paper, we have substituted the motor domain of Kin:βgal...

... by Company of Biologists 1996 Xenopus laevis dorsal determinants cortical rotation localization axis specification Establishment of bilateral symmetry that defines the future dorsoventral axis of the embryo is an essential event of early animal development. In Xenopus , the egg...

... localization pattern, the predicted PEM protein shows no significant homology to known proteins. Overexpression of this gene by microinjection of synthesized pem mRNA into fertilized eggs results in development of tadpole larvae with deficiency of the anteriormost adhesive organ, dorsal brain and sensory...

...Finn-Hugo Markussen; Anne-Marie Michon; Wolfgang Breitwieser; Anne Ephrussi ABSTRACT At the posterior pole of the Drosophila oocyte, oskar induces a tightly localized assembly of pole plasm. This spatial restriction of oskar activity has been thought to be achieved by the localization of oskar mRNA...

...Joel K. Yisraeli; Sergei Sokol; D. A. Melton In an effort to understand how polarity is established in Xenopus oocytes, we have analyzed the process of localization of the maternal mRNA, vg1. In fully grown oocytes, vg1 mRNA is tightly localized at the vegetal cortex. Biochemical fractionation...

...D. Tannahill; D. A. Melton ABSTRACT The Xenopus Vgl gene encodes a maternal mRNA that is localized to the vegetal hemisphere of both oocytes and embryos and encodes a protein related to the TGF-β family of small secreted growth factors. We have raised antibodies to recombinant Vgl protein and used...

... % of the pyrimidine bases. Rescuing activity is present in cytoplasm until the blastoderm stage but disappears earlier from poly(A) + RNA. Rescuing activity is also present, and localized, in cytoplasm of embryos from two related dipterans, Smittia sp. and Drosophila melanogaster , although the extent of rescue...