...Geoffrey Soussi; Ausra Girdziusaite; Shalu Jhanwar; Victorio Palacio; Marco Notaro; Rushikesh Sheth; Rolf Zeller; Aimée Zuniga ABSTRACT During limb bud formation, axis polarities are established as evidenced by the spatially restricted expression of key regulator genes. In particular, the mutually...

...Jean-Denis Bénazet; Emanuele Pignatti; Ashleigh Nugent; Erkan Unal; Frédéric Laurent; Rolf Zeller SMAD4 is an essential mediator of canonical TGFβ/BMP signal transduction and we inactivated Smad4 in mouse limb buds from early stages onward to study its functions in the mesenchyme. While this Smad4...

...Laurie A. Wyngaarden; Paul Delgado-Olguin; I-hsin Su; Benoit G. Bruneau; Sevan Hopyan Specification and determination (commitment) of positional identities precedes overt pattern formation during development. In the limb bud, it is clear that the anteroposterior axis is specified at a very early...

...Laurie A. Wyngaarden; Kevin M. Vogeli; Brian G. Ciruna; Mathew Wells; Anna-Katerina Hadjantonakis; Sevan Hopyan The vertebrate limb bud arises from lateral plate mesoderm and its overlying ectoderm. Despite progress regarding the genetic requirements for limb development, morphogenetic mechanisms...

...Kosei Sato; Yutaka Koizumi; Masanori Takahashi; Atsushi Kuroiwa; Koji Tamura Pattern formation along the proximal-distal (PD) axis in the developing limb bud serves as a good model for learning how cell fate and regionalization of domains, which are essential processes in morphogenesis during...

... in differentiating embryonic stem (ES) cells and in the tailbud of the embryo. However, looping out and decondensation are not simply two different manifestations of the same underlying change in chromatin structure. We show that, in the limb bud of the embryonic day 9.5 embryo,where Hoxd is also activated...

... Sirenomelia Twisted gastrulation (Twsg1) Bmp Bmp4 Morpholino Limb bud TGFβ Chordin Dorsoventral patterning Mouse Xenopus Sirenomelia is a human developmental malformation that has been a source of enduring interest. The abnormal fetuses are characterized by the fusion of both legs...

...Odyssé Michos; Lia Panman; Kristina Vintersten; Konstantin Beier; Rolf Zeller; Aimée Zuniga Epithelial-mesenchymal feedback signaling is the key to diverse organogenetic processes such as limb bud development and branching morphogenesis in kidney and lung rudiments. This study establishes...

... expression in chick limb bud cells in culture. Dev. Growth Differ . 40 , 457 -464. Li, Q. Y., Newbury-Ecob, R. A., Terrett, J. A., Wilson, D. I., Curtis, A. R., Yi, C. H., Gebuhr, T., Bullen, P. J., Robson, S. C., Strachan, T., Bonnet, D., Lyonnet, S., Young, I. D., Raeburn, J. A., Buckler, A. J...

... that of MyoD . Moreover, Myf5 and Pax3 expression is correlated with myoblast proliferation, while MyoD is detected in post-mitotic myoblasts. These data indicate that Myf5 and MyoD are involved in different steps during chick limb bud myogenesis, Myf5 acting upstream of MyoD . The progression of myoblasts...

...-specific manner by interacting with the limb mesenchyme and the cartilage elements. The molecular mechanisms that provide positional cues to the muscle precursors are still unknown. We have shown that the expression patterns of Hoxa-11 and Hoxa-13 are correlated with muscle patterning of the limb bud...

...Aimée Zúñiga; Rolf Zeller During initiation of limb-bud outgrowth in vertebrate embryos, the polarising region (limb-bud organizer) is established upon activation of the Sonic Hedgehog (SHH) signaling molecule at the posterior limb-bud margin. Another hallmark of establishing anteroposterior limb...

... on several interacting signaling centers; one of these, the zone of polarizing activity (ZPA), comprises a group of mesenchymal cells along the posterior aspect of the limb bud that express sonic hedgehog ( Shh ) and plays a key role in patterning the anterior-posterior (AP) axis. The mechanisms by which...

...Masakazu Yamamoto; Yoshihiro Gotoh; Koji Tamura; Mikiko Tanaka; Atsushi Kawakami; Hiroyuki Ide; Atsushi Kuroiwa ABSTRACT The limb muscle precursor cells migrate from the somites and congregate into the dorsal and ventral muscle masses in the limb bud. Complex muscle patterns are formed...

.... Myogenic cells in limb skeletal muscles originate from the lateral half of the somites. Cells that migrate away from the lateral part of the somites to the limb bud do not initially express any member of the MRF family. Expression of MRFs in the muscle precursor cells starts after the migration process...

... activity. The results reveal a gain-of-function mutation at a locus that mediates pattern formation in the developing limb. Shh expression is identical with that of wild-type embryos, i.e. there is no ectopic expression. However, mesenchyme from the anterior aspects of Dbf/+ mutant limb buds, when...

... was identified as a putative regulator of D–V patterning in the developing vertebrate limb based on its restricted expression in dorsal limb bud ectoderm ( Dealy et al., 1993 ; Parr et al., 1993 ). In the mouse, Wnt-7a transcripts are present in the dorsal ectoderm prior to limb outgrowth and remain dorsally...

... on an earlier stage (HH13). Using the quail-chick chimera system, we first show that the presumptive wing mesoderm occupies the medial half of the somatopleure at the level of somites 15-20. The corresponding ectodermal area, however, will only give rise to the apical ectodermal ridge. The rest of the limb bud...

...Yuji Yokouchi; Jyun-ichi Sakiyama; Takashi Kameda; Hideo Iba; Atsushi Suzuki; Naoto Ueno; Atsushi Kuroiwa ABSTRACT During limb development, the mesenchymal cells in restricted areas of limb bud, anterior necrotic zone, posterior necrotic zone, opaque zone and interdigital necrotic zones...

.... M. are both first authors 19 5 1996 © 1996 by Company of Biologists 1996 TGFβ apoptosis growth factor Msx-1 Msx-2 ck-erg BMP-4 cell death chick limb bud autopod The developing limb bud is a relatively simple structure consisting of mesoderm covered...