... zebrafish embryos exhibit aberrant apical extrusion of ventricular cardiomyocytes. While investigating apical cardiomyocyte extrusion, we identified a basal-to-apical shift in laminin deposition from the internal to the external ventricular wall. We find that epicardial cells express several laminin...

... be demonstrated by depleting laminin, which causes normally quiescent embryonic PGCs to proliferate. We show that following laminin depletion, niche cells relay proliferation-inducing signals from the gonadal BM to PGCs via integrin receptors. Disrupting the BM proteoglycan perlecan blocks PGC proliferation when...

...Saptarshi Biswas; Sanjid Shahriar; Nicholas P. Giangreco; Panos Arvanitis; Markus Winkler; Nicholas P. Tatonetti; William J. Brunken; Tyler Cutforth; Dritan Agalliu ABSTRACT Neurovascular unit and barrier maturation rely on vascular basement membrane (vBM) composition. Laminins, a major vBM...

... to radially sort and myelinate peripheral axons. Elevation of cAMP, but not Rac1 activity, in csp −/− restores myelin ensheathment. Most importantly, we show a significant decrease in laminin expression within csp −/− posterior lateral line nerve and that forcing Laminin 2 expression in csp −/− fully restores...

... composition and mechanical properties of the basement membrane underlying the follicle epithelium of Drosophila egg chambers. We find that the Laminin and Collagen IV networks largely persist in the absence of the other components. Moreover, we show that Perlecan and Collagen IV, but not Laminin or Nidogen...

... simultaneously with growth of the heart. Cardiac growth during early heart morphogenesis is driven by deployment of cells from the second heart field (SHF) into both poles of the heart. Laminin is a core component of the extracellular matrix and, although mutations in laminin subunits are linked with cardiac...

... and α6/β4 are the mammary epithelial cell receptors for laminins, which are major components of the mammary basement membrane. The roles of specific basement membrane components and their integrin receptors in the regulation of functional gland development have not been analyzed in detail. To investigate...

...Maqsood Ahmed; Leandro N. Marziali; Ernest Arenas; M. Laura Feltri; Charles ffrench-Constant ABSTRACT Development of the central nervous system requires coordination of the proliferation and differentiation of neural stem cells. Here, we show that laminin alpha 2 (lm-α2) is a component...

...Georg Wolfstetter; Ina Dahlitz; Kathrin Pfeifer; Uwe Töpfer; Joscha Arne Alt; Daniel Christoph Pfeifer; Reinhard Lakes-Harlan; Stefan Baumgartner; Ruth H. Palmer; Anne Holz ABSTRACT Basement membranes (BMs) are specialized layers of extracellular matrix (ECM) mainly composed of Laminin, type IV...

... interests The authors declare no competing or financial interests. 25 9 2015 18 2 2016 Here we show that depletion of Laminin from muscle sheath BM causes a progressive, dystrophic phenotype in this tissue. We then use the resulting changes in muscle sheath contractility to probe...

... morphology along the proximo-distal axis. This epithelial pattern is strictly correlated with the gradient of canonical Wnt signalling activity. By combining genetic analyses with cellular imaging, we show that canonical Wnt signalling regulates epithelial cell morphology by modulating the levels of laminins...

.... We identified a mutation affecting zebrafish laminin β1a ( lamb1a ) that disrupts left-right asymmetry of the liver and pancreas. In these mutants, the liver spans the midline and the ventral pancreatic bud remains split into bilateral structures. We show that lamb1a regulates asymmetric left-right...

... a laminin-containing basement membrane - the inner limiting membrane. Genetic deletion of the laminin β2 and γ3 chains affects astrocyte migration and spatial distribution. We show that laminins act as haptotactic factors in vitro in an isoform-specific manner, inducing astrocyte migration and promoting...

... polarize with apical localization of the PAR-3 protein complex, then undergo apical constriction to form a cylindrical cyst. We show that laminin, but not other basement membrane components, orients the polarity of the double plate cells. Our results provide in vivo evidence that laminin has an early role...

...Jose M. Urbano; Catherine N. Torgler; Cristina Molnar; Ulrich Tepass; Ana López-Varea; Nicholas H. Brown; Jose F. de Celis; Maria D. Martín-Bermudo Laminins are heterotrimeric molecules found in all basement membranes. In mammals, they have been involved in diverse developmental processes, from...

...Claire Anderson; Sólveig Thorsteinsdóttir; Anne-Gaëlle Borycki Basement membranes have essential structural and signalling roles in tissue morphogenesis during embryonic development, but the mechanisms that control their formation are still poorly understood. Laminins are key components...

... function locally promotes basement membrane dissolution in the primary mouth primordium. Consistently, Frzb-1 overexpression decreases RNA levels of the essential basement membrane genes fibronectin and laminin ,whereas Wnt-8 overexpression increases the levels of these RNAs. These data are the first...

...,Ontario, Canada). * Author for correspondence (e-mail: [email protected] ) 21 11 2008 © 2009. 2009 Oligodendroglia Myelination Myelin Netrin Integrin Laminin Autocrine Mouse Rat FAK (Ptk2) N-WASP (Wasl) To form a myelin sheath, an oligodendrocyte...

...Holly Colognato; Jason Galvin; Zhen Wang; Jenne Relucio; Tom Nguyen; David Harrison; Peter D. Yurchenco; Charles ffrench-Constant Developmental abnormalities of myelination are observed in the brains of laminin-deficient humans and mice. The mechanisms by which these defects occur remain unknown...

...) as a model system, we show that the ECM molecule Perlecan(Pcan) is required for maintenance of epithelial-cell polarity. Follicle cells that lack Pcan develop polarity defects similar to those of Dg mutant cells. Furthermore, Dg depends on Pcan but not on Laminin A for its localization in the basal-cell...