... intracellular pH (pH i ) in Xenopus gastrula cells via the fluorescence ratio technique using the pH sensitive fluorophore BCECF. Ectoderm and neurectoderm explants from different developmental stages were loaded with the membrane permeant ester BCECF-AM and fluorescence imaging then revealed that nearly all...

... been microinjected during cleavage stages with the fluorescent pH indicator bis-carboxyethyl-carboxyfluores-cein-dextran (BCECF-dextran), and intracellular pH (pH i ) was monitored continuously by emission ratio microfluo-rimetry. During stage 10.5, the dorsal ectoderm cells undergo a sustained...

...Nathalie Grandin; Michel Charbonneau ABSTRACT Cell activation during fertilization of the egg of Xenopus laevis is accompanied by various metabolic changes, including a permanent increase in intracellular pH (pHi) and a transient increase in intracellular free calcium activity ([Ca 2+ ] 1...

...Michiel M. Van Lookeren Campagne; Rob J. Aerts; Wouter Spek; Richard A. Firtel; Pauline Schaap ABSTRACT Prespore gene expression in Dictyostelium is induced by the interaction of cAMP with cell surface cAMP receptors. We investigated whether intracellular pH (pH i ) changes mediate the induction...

... of Biologists 1988 chromosome condensation cytostatic factor interspecific microinjection intracellular pH maturation promoting factor meiosis reinitiation Patella vulgata protein phosphorylation protein synthesis GV prophase-arrested oocytes of most animals are triggered to resume...

... defined by such experiments is not known, but two factors which have been proposed to play a role in regulating protein synthesis are ribosomal protein S6 phosphorylation and intracellular pH. In the current paper, we review the literature and present new evidence on the roles intracellular pH and S6...