...Julia K. Grzymkowski; Yu-Chun Chiu; Dereje D. Jima; Brent H. Wyatt; Sudhish Jayachandran; Whitney L. Stutts; Nanette M. Nascone-Yoder ABSTRACT Malrotation of the intestine is a prevalent birth anomaly, the etiology of which remains poorly understood. Here, we show that late-stage exposure...

...Flora Demouchy; Ophélie Nicolle; Grégoire Michaux; Anne Pacquelet ABSTRACT The kinase PAR-4/LKB1 is a major regulator of intestinal homeostasis, which prevents polyposis in humans. Moreover, its ectopic activation is sufficient to induce polarization and formation of microvilli-like structures...

... cells into human intestinal organoids (HIOs) has served as a powerful means for creating complex three-dimensional intestinal structures. Owing to their diverse cell populations, transplantation into an animal host is supported with this system and allows the temporal formation of fully laminated...

...Anne M. Bara; Lei Chen; Celina Ma; Julie Underwood; Rebecca S. Moreci; Kaelyn Sumigray; Tongyu Sun; Yarui Diao; Michael Verzi; Terry Lechler ABSTRACT There are fundamental differences in how neonatal and adult intestines absorb nutrients. In adults, macromolecules are broken down into simpler...

... protein depletion in the Caenorhabditiselegans embryonic intestine, we found that local and global polarity establishment are temporally and genetically separable. Local polarity is initiated prior to global polarity and is robust to perturbation. PAR-3 is required for global polarization across...

... aspects of intestinal morphogenesis: enteric nervous system formation, epithelium structuring, muscle orientation and differentiation, anisotropic growth and the development of myogenic and neurogenic motility. I outline numerous implications of this biomechanical perspective in the etiology and treatment...

... and reliable tool. Although we primarily focus on recent findings enabled by intestinal organoids co-cultured with lymphocytes, we posit that organoid co-culture systems will support future efforts to disentangle the interactions between a plethora of different cell types throughout development, homeostasis...

...Aurélien Bidaud-Meynard; Flora Demouchy; Ophélie Nicolle; Anne Pacquelet; Shashi Kumar Suman; Camille N. Plancke; François B. Robin; Grégoire Michaux ABSTRACT The intestinal brush border is made of an array of microvilli that increases the membrane surface area for nutrient processing, absorption...

...Cristina Brischetto; Karsten Krieger; Christian Klotz; Inge Krahn; Séverine Kunz; Marina Kolesnichenko; Patrick Mucka; Julian Heuberger; Claus Scheidereit; Ruth Schmidt-Ullrich ABSTRACT Although the role of the transcription factor NF-κB in intestinal inflammation and tumor formation has been...

...Meghan Ferguson; Kristina Petkau; Minjeong Shin; Anthony Galenza; David Fast; Edan Foley ABSTRACT Microbial factors influence homeostatic and oncogenic growth in the intestinal epithelium. However, we know little about immediate effects of commensal bacteria on stem cell division programs...

... cell nearby. It is possible that these two cells might be produced via a cell division. Daughter cells after division move apart from each other by ∼20-70 µm in the intestine during normal development through 89-107 hours postfertilization ( Kuwata et al., 2019 ). We next quantified the ratios of GFP...

... body axis are beginning to be understood, the mechanisms that shape the asymmetries of individual organs remain less clear. Here, we summarize new evidence challenging century-old ideas about the development of stomach and intestine laterality. We compare classical and contemporary models of asymmetric...

...Brett R. Lancaster; James D. McGhee ABSTRACT We define a quantitative relationship between the affinity with which the intestine-specific GATA factor ELT-2 binds to cis -acting regulatory motifs and the resulting transcription of asp-1 , a target gene representative of genes involved...

... reporter activity correlates with proliferating regions of the brain, haematopoietic tissue and intestine. In the adult gut, the reporter is active in sparse proliferating cells, located at the base of intestinal folds, expressing the stemness marker sox9b and having the morphology of mammalian crypt base...

...Robert J. Garriock; Ravindra B. Chalamalasetty; JianJian Zhu; Mark W. Kennedy; Amit Kumar; Susan Mackem; Terry P. Yamaguchi ABSTRACT Despite the importance of Wnt signaling for adult intestinal stem cell homeostasis and colorectal cancer, relatively little is known about its role in colon formation...

... Imaginal disc Intestine Regeneration National Institutes of Health 10.13039/100000002 GM118447 GM107140 In the early 1900s, Thomas Hunt Morgan turned his research focus to examine the genetic basis of heredity in the organism Drosophila , moving away from previous efforts...

...Aurélien Bidaud-Meynard; Ophélie Nicolle; Markus Heck; Yann Le Cunff; Grégoire Michaux ABSTRACT Intestine function relies on the strong polarity of intestinal epithelial cells and the array of microvilli forming a brush border at their luminal pole. Combining a genetic RNA interference (RNAi...

...Namit Kumar; Yu-Hwai Tsai; Lei Chen; Anbo Zhou; Kushal K. Banerjee; Madhurima Saxena; Sha Huang; Natalie H. Toke; Jinchuan Xing; Ramesh A. Shivdasani; Jason R. Spence; Michael P. Verzi ABSTRACT Lineage-restricted transcription factors, such as the intestine-specifying factor CDX2, often have dual...

... that it also regulates critical homeostatic processes in germline and somatic stem cells, as well as regenerative processes in several tissues, including the gonad, intestine and appendages. Here, we provide an overview of JAK/STAT signaling in stem cells and regeneration, focusing on Drosophila...

... mechanisms of cell fate regulation through niche-derived cues, with a particular focus on epithelial stem cells of the mammalian skin, intestine and lung. We discuss a spectrum of niche-derived biochemical, mechanical and architectural inputs that define stem cell states during morphogenesis, homeostasis...