.... Summary: Loss of NR5A1 after the sex determination period causes Sertoli cells to revert to supporting progenitor-like cells and transdifferentiate into pre-granulosa-like cells that die by a Trp53 -independent mechanism. Gonad Integrin Leydig cell Mutant Testis NR5A1/SF-1 Centre...

... be demonstrated by depleting laminin, which causes normally quiescent embryonic PGCs to proliferate. We show that following laminin depletion, niche cells relay proliferation-inducing signals from the gonadal BM to PGCs via integrin receptors. Disrupting the BM proteoglycan perlecan blocks PGC proliferation when...

... junctions. We identified integrin signalling as a key pathway downstream of MAPK/ERK in the tip progenitors; disrupting integrin alters polarity, cell adhesion and tight junction assembly. By contrast, stimulation with FGF10 or EGF alone is not sufficient to maintain organoid columnar cell shape. This study...

... in cardiomyocyte cell cycle arrest, we conditionally activated ROCK2 kinase domain (ROCK2:ER) in the murine postnatal heart. Here, we show that α5/β1 integrin and fibronectin matrix increase in response to actomyosin-mediated tension. Moreover, activation of ROCK2:ER promotes nuclear translocation of Yap...

... and cell migration in vivo . Finally, we conclude by discussing similarities and differences in how FAs function in vitro and in vivo . The requirement for integrin in enteric neural crest cells to invade the large intestine is also reflected in differential ECM composition; the mesenchyme of the large...

... controls cell shape and mechanical tension to guide the morphogenesis of Drosophila wing discs. Epithelial folding Mechanical tension Integrin Wnt/Wingless Wing disc Drosophila Animals undergo a series of shape changes during development to reach their final form. The reshaping...

...Mathilde Romagnoli; Laura Bresson; Amandine Di-Cicco; María Pérez-Lanzón; Patricia Legoix; Sylvain Baulande; Pierre de la Grange; Adèle De Arcangelis; Elisabeth Georges-Labouesse; Arnoud Sonnenberg; Marie-Ange Deugnier; Marina A. Glukhova; Marisa M. Faraldo ABSTRACT Integrin dimers α3/β1, α6/β1...

... progenitors in a proliferative state via integrins α6β1 and α7β1, whereas low concentrations of lm211 support mDA lineage survival via dystroglycan receptors. We confirmed our findings in vivo , demonstrating that the VM was smaller in the absence of lm-α2, with increased apoptosis; furthermore...

... TVM morphogenesis; however, the inverse, supplying FGF to the TVM, does not rescue CVM mismigration. In addition, we show that FGF regulates integrin expression in both tissues, but only providing a source of integrin specifically to the TVM attenuates the contralateral merging phenotype. Finally, we...

... ; Chatzeli et al., 2017 ; Seymour et al., 2012 ; Volckaert et al., 2013 ), and overexpression of Fgf10 is sufficient to drive β-catenin activation ( Volckaert et al., 2013 , 2011 ) and nuclear Yap. Our data demonstrate that mechanical cues via an ECM-integrin-ILK-Merlin-Hippo-Yap-β-catenin-Fgf10...

... transition (MET). FGF activity leads to accumulation of β-integrin Myospheroid basally and cell polarity factor Bazooka apically within mesoderm cells, thereby reestablishing apicobasal cell polarity in an epithelialized state in which cells express both E-Cadherin and N-Cadherin. In summary, FGF plays...

... that a fibronectin-integrin-syndecan module acts between p21-activated kinase 1 upstream and ephrin B1 downstream to promote the contact-induced collapse of lamellipodia. To function in this module, fibronectin has to be present as puncta on the surface of LEM cells. To overcome contact inhibition in LEM cell...

...-structured functional tissues. Using the planarian Schmidtea mediterranea as a model, we identify β1-integrin as a crucial regulator of blastema architecture. β1-integrin(RNAi) animals formed small head blastemas with severe tissue disorganization, including ectopic neural spheroids containing differentiated...

... in adult mice slows proliferation of basal layer cells, leads to hair loss and impairs regeneration after wounding. Contact with the basal extracellular matrix and consequent integrin-Src signalling is a key determinant of the nuclear localisation of YAP/TAZ in basal layer cells and in skin tumours...

... transversum. Utilizing a small-molecule screen, we found that Lhx9 acts upstream of integrin-paxillin signaling and consistently demonstrate that either loss of Lhx9 or disruption of the integrin-paxillin pathway results in mis-positioning of the proepicardial organ and aberrant deposition of extracellular...

... that Crim1 acts in LE cells, where it colocalizes with and regulates the levels of active β1 integrin and of phosphorylated FAK and ERK. The RGD and transmembrane motifs of Crim1 are required for regulating FAK phosphorylation. These results identify an important function for Crim1 in the regulation...

...Christopher J. Turner; Kwabena Badu-Nkansah; Denise Crowley; Arjan van der Flier; Richard O. Hynes The RGD-binding α5 and αv integrins have been shown to be key regulators of vascular smooth muscle cell (vSMC) function in vitro . However, their role on vSMCs during vascular development in vivo...

...Xiaojun Xie; Mary Gilbert; Lindsay Petley-Ragan; Vanessa J. Auld Many aspects of glial development are regulated by extracellular signals, including those from the extracellular matrix (ECM). Signals from the ECM are received by cell surface receptors, including the integrin family. Previously, we...

... processes, but how netrin signaling is coordinated with other pathways during invasion is poorly understood. Using the model of anchor cell (AC) invasion in C. elegans , we have previously shown that the integrin receptor heterodimer INA-1/PAT-3 promotes netrin receptor UNC-40 (DCC) localization...

... demonstrate that ephrin B1 is required in apical progenitors (APs) to maintain their apical adhesion. Mechanistically, we show that ephrin B1 controls cell-ECM adhesion by promoting apical localization of integrin β1 and we identify ADP-ribosylation factor 6 (Arf6) as an important effector of ephrin B1...