... the established epiblast, or production of both tissues from the inner cell mass precursor. To begin to resolve this discrepancy and thereby understand the sequential process for production of viable human embryos, we investigated the expression order of genes associated with emergence of hypoblast. Based upon...

... work is properly attributed. Highlighted Article: Analysis of stage-matched, single-cell gene expression data from three mammalian species reveals conserved and primate-specific regulation of early embryogenesis and lineage specification. Blastocyst Embryo Human Inner cell mass...

...Takuya Azami; Tsuyoshi Waku; Ken Matsumoto; Hyojung Jeon; Masafumi Muratani; Akihiro Kawashima; Jun Yanagisawa; Ichiro Manabe; Ryozo Nagai; Tilo Kunath; Tomonori Nakamura; Kazuki Kurimoto; Mitinori Saitou; Satoru Takahashi; Masatsugu Ema The inner cell mass of the mouse blastocyst gives rise...

...Tomoko Watanabe; John S. Biggins; Neeta Bala Tannan; Shankar Srinivas The formation of trophectoderm (TE) and pluripotent inner cell mass (ICM) is one of the earliest events during mammalian embryogenesis. It is believed that the orientation of division of polarised blastomeres in the 8- and 16...

...Minjung Kang; Anna Piliszek; Jérôme Artus; Anna-Katerina Hadjantonakis The emergence of pluripotent epiblast (EPI) and primitive endoderm (PrE) lineages within the inner cell mass (ICM) of the mouse blastocyst involves initial co-expression of lineage-associated markers followed by mutual exclusion...

... declare no competing financial interests. © 2012. AP axis Blastocyst Cell fate Trophectoderm Inner cell mass Primitive endoderm The early development of the mouse embryo, from a fertilized egg to a gastrulating embryo, involves a tightly regulated series of lineage...

...Robert Odell Stephenson; Yojiro Yamanaka; Janet Rossant The first two cell lineages in the mouse, the surface trophectoderm (TE) and inner cell mass (ICM), are morphologically distinguishable by E3.5, with the outer TE forming a polarized epithelial layer enclosing the apolar ICM. We show here...

...Anne K. Voss; Tim Thomas; Petros Petrou; Konstantinos Anastassiadis; Hans Schöler; Peter Gruss ABSTRACT The cells of the inner cell mass constitute the pluripotent cell population of the early embryo. They have the potential to form all of the tissues of the embryo proper and some extra-embryonic...

... ). Differentiation of the inner cell mass of the baboon blastocyst . Anat. Rec . 226 , 237 – 248 . 10.1002/ar.1092260213 ffrench-Constant , C. , Hollingsworth , A. , Heasman , J. and Wylie , C. C. ( 1991 ). Response to fibronectin of mouse primordial germ cells before, during...

... into the inner cell mass (ICM). Secondly, as trophectoderm generates the blastocoele by vectorial fluid transport, this epithelium segregates into polar (proliferative) and mural (non-proliferative) subpopulations. Thirdly, the ICM segregates into primary endoderm (epithelium; at blastocoele interface...

... trophectoderm lineage from compaction (8-cell stage) onwards, ultimately forming a zonular belt around each trophectoderm cell of the blastocyst (32- to 64-cell stage). The protein is usually undetectable within the inner cell mass (ICM) although, in a minority of embryos, punctate ZO-1 sites are present...

.... Total, trophectoderm (TE) and inner cell mass (ICM) cell numbers were analyzed by differential labelling of the nuclei with polynucleotide specific fluorochromes. The TE nuclei were labelled with one fluorochrome during immunosurgical lysis, before fixing the embryo and labelling both sets of nuclei...

...Jonathan Dyce; Martin George; Harry Goodall; Tom P. Fleming ABSTRACT The extent to which trophectoderm (TE) and inner cell mass (ICM) lineages in the mouse blastocyst remain distinct during the period from the commencement of cavitation up until 48 h later in culture was investigated. Fluorescent...

..., Ontario, M5G 1X5, Canada. 05 08 1986 Copyright © 1986 by Company of Biologists 1986 retinoic acid embryonal carcinoma inner cell mass chimaera mouse Murine embryonal carcinoma (EC) cells, the stem cells of teratocarcinomas derived from embryos or germ cells, can...

...Hester P. M. Pratt ABSTRACT The preimplantation mouse blastocyst consists of two differentiated tissues, the troph-ectoderm (a structurally and functionally polarized epithelium) and the inner cell mass. The divergence of these two cell types can be traced back to a contact dependent polarization...

...Sheila C. Barton; Catharine A. Adams; M. L. Norris; M. A. H. Surani ABSTRACT The developmental potential of inner cell mass (ICM) and trophectoderm (TE) derived from parthenogenetic or biparental gynogenetic embryos was examined in reconstituted blastocysts with normal TE or ICM, respectively...