... copies of these tags allowed efficient POI binding and manipulation in living cells. Using Drosophila , we also find that single short tags can be used for POI manipulation in vivo . * These authors contributed equally to this work ‡ Authors for correspondence ( [email protected]...

... extrapolated from in vitro and ex vivo studies, because in vivo analysis of sperm in their native fertilization environment has not been possible. Here, we report a functional optical coherence tomography approach that allows, for the first time, in vivo three-dimensional tracking of sperm behaviors...

... characterization of adipocytes during development using DNA microarrays. These data reveal distinct transcriptional programs that are regulated during murine WAT development in vivo. By using a de novo cis-regulatory motif discovery tool (FIRE), we identify two early gene clusters whose promoters show significant...

... of Dap, respectively. Finally, we show that the fate determinant factor, Tramtrack, downregulates Cyclin E expression and is probably involved in the exit of the cells from the cell cycle. BrdU Dacapo G1-phase In vivo Microchaete Tramtrack Drosophila In several well-studied systems...

... at the tips of these neuronal processes. Although the behavior of growth cones has been well-characterized in vitro, it is difficult to observe growth cones in vivo. We have observed motor neuron growth cones migrating in living Caenorhabditis elegans larvae using time-lapse confocal microscopy. Specifically...

... axial muscle in vivo cell fate zebrafish muscle fiber type muscle pioneer cell migration The properties and distributions of different fiber types within vertebrate muscles have been well characterized, however, the mechanisms that regulate the initial development of fiber type differences...

... genes as well as the ecdysone receptor ( EcR ) present varying dose response characteristics ( Karim and Thummel, 1992 , EMBO J . 11, 4083–4093). We have developed an in vivo approach using a reverse transcription-polymerase chain reaction assay (RT-PCR) so as to visualise these transcripts in the RNA...

... interactions with nuclear DNA-binding proteins of the embryo. This gene is activated in late cleavage, exclusively in cells of the aboral ectoderm cell lineages. In this study, we investigate the functional importance in vivo of these sites of DNA-protein interaction. Sea urchin eggs were coinjected...

...Sandra A. Rempel; Randal N. Johnston ABSTRACT Enhanced c-myc transcript abundance has been observed in a variety of human malignancies, in normal liver tissue induced to proliferate in vivo by partial hepatectomy and in cells in culture induced to proliferate with the addition of protein hormones...

...Hermann Rohrer; Magdalena Hofer; Rainer Hellweg; Sigrun Korsching; August D. Stehle; Siawusch Saadat; Hans Thoenen The monoclonal antibody 27/21 directed against mouse nerve growth factor (NGF) interferes in vivo with the survival of sensory dorsal root ganglion (DRG) neurones during...

... differentiation of ICM and trophectoderm in early blastocysts. The proportion of ICM cells then decreased as blastocysts expanded and hatched. Some embryos were cultured in vitro and others were transferred to the oviducts of immature mice as a surrogate in vivo environment and assessed for morphology and cell...

... are formed by back branching rather than by bifurcation of leading growth cones. In a second experiment, labelled growing axons were separated from their cell bodies by removing the retina. Such isolated axons continued to grow for up to 3h in vivo and were capable of recognizing the tectum and arborizing...