... transcriptomics, readily applicable to a wide range of tissue types and model organisms. Spatial transcriptomics In situ hybridization Multiplex imaging Multi-omics Open source Padlock probes Chan Zuckerberg Initiative Silicon Valley Community Foundation http://dx.doi.org...

...; David A. Prober; Matt van de Rijn; Jared R. Leadbetter; Dianne K. Newman; Carol Readhead; Marianne E. Bronner; Barbara Wold; Rusty Lansford; Tatjana Sauka-Spengler; Scott E. Fraser; Niles A. Pierce In situ hybridization methods are used across the biological sciences to map mRNA expression within intact...

... is disorganized in the Olig2-KO dorsal thalamus, and initial elongation of TCAs is retarded in the Olig2-KO forebrain. Microarray analysis demonstrated upregulation of several axon guidance molecules, including Epha3 and Epha5 , in the Olig2-KO basal forebrain. In situ hybridization showed that the prethalamus...

... ) * These authors contributed equally to this work 14 2 2005 ©2005. 2005 Planarians Regeneration Neoblasts Stem cells RNAi Cell proliferation Pumilio PUF proteins Confocal microscopy TEM Cytofluorimetry In situ hybridization Post-transcriptional regulation Stem cells...

.... Fig. 6. Expression of Rock in mouse at E11-E13. At E11 (A,A′), Rock1 mRNA could be detected by in situ hybridization in the ventricular (short arrow) and subventricular zone (large arrow) of the rhombic lip. Note the presence of Rock1 -positive cells in the early migratory pathway (arrowheads...

.... The expression pattern of Pax6A and Pax6B was analyzed by whole-mount in situ hybridization in intact adult specimens of both species. The presence of Pax6A transcripts was observed in the cephalic ganglia and in the nerve cords that cross the entire organism ventrally. ( Fig. 3 A-G). Pax6B showed...

... ). In situ hybridization analysis of chick embryos in whole mount and tissue sections . In Methods in Avian Embryology . Methods in Cell Biology , vol. 51 , (ed. M. Bronner-Fraser ). New York : Academic Press, Inc . Nieuwkoop , P. D. ( 1969 ). he formation of the mesoderm in urodelan...

... Forest, IL 60045, USA 17 03 1999 04 05 1999 © 1999 by Company of Biologists 1999 Blastoderm Epiblast Gastrulation Hensen’s node In situ hybridization Neural plate Neural tube Neurulation Organizer Primitive streak Quail/chick chimera It is conceivable...

... repeats, DNA-binding domains, SH3-binding motifs and nuclear localization signals, are highly conserved. Northern blot analyses detect a single message of 8.9 and 5.8 kilo base (kb) from both cardiac and skeletal muscle of chick and mouse, respectively. In situ hybridization reveals that the cXin gene...

... Biology and Anatomy, Medical University of South Carolina, Charleston, SC 29425, USA † Author for correspondence (e-mail: [email protected] ) 07 11 1997 13 01 1998 © 1998 by Company of Biologists 1998 Retinoids Heart cDNA subtraction In situ hybridization Energy depletion...

... Wilkinson , D. G. ( 1992 ). In Situ Hybridization. A Practical Approach . Oxford : IRL Press at Oxford University Press . Yamada , T. , Placzek , M. , Tanaka , H. , Dodd , J. and Jessell , T. M. ( 1991 ). Control of cell pattern in the developing nervous system...

.../+ crosses. β-actin probe was provided by Janet Rossant. Both the Pax-3 and Pax-6 probes were the same as used for in situ hybridization. The assay was conducted as described by Ausubel et al. (1987) , using 10 µg of total cerebellar RNA and 5×105 cpm of probe RNA in each reaction. Quantitation...

.... Within the early gastrula Organizer, the genes Xbra, goosecoid, noggin and xNR3 are expressed differently in the animal-vegetal and superficial-deep dimensions. In situ hybridization and lineage labelling define distinct regions of the dorsal marginal zone. By the end of gastrulation, dorsal axial...

... for introducing us to the whole-mount in situ hybridization technique. REFERENCES Amakawa , R. , Wu , J. , Ozawa , K. , Matsunami , N. , Hamaguchi , Y. , Matsuda , F. , Kawaichi , M. and Honjo , T. ( 1993 ). Human JК recombination signal binding protein gene (IGKJRB...

... of the mammary secretory phenotype. By in situ hybridization, we have established evidence for asynchrony in milk protein gene expression among alveolar cells showing large variations in the intensity of hybridization among adjacent cells. The asynchrony of maturation of epithelial cells within a given alveolus...

..., by northern analysis and in situ hybridization with 33 P-labelled antisense probes to each of the three mouse zona mRNAs, we have determined that the expression of each mouse zona gene is restricted to the oocyte. ZP2 transcripts, but not ZP1 or ZP3, are detected in resting (15 μm diameter) oocytes, and all...

... and neonatal mammalian stomach. TGFα has been localized by in situ hybridization and by immunohistochemical visualization to the mature, non-proliferative compartment of the gastric mucosa, suggestive of a role in cellular differentiation ( Beauchamp et al., 1989 ; Thomas et al., 1992 ; Nasim et al., 1992...

...A. Feijen; M. J. Goumans; A. J. M. van den; Eijnden-van Raaij ABSTRACT Using in situ hybridization we have studied the localization of the messenger RNAs encoding the inhibin/activin subunits (α, βA, βB), the activin-binding protein follistatin and activin receptors (IIA, IIB) in mouse embryos...

...Geraldine Seydoux; Andrew Fire ABSTRACT Early embryogenesis in Caenorhabditis elegans is characterized by a series of unequal cleavages that mark the stepwise separation of somatic and germ lineages. We have developed an in situ hybridization protocol to examine the localization of specific...

... by Company of Biologists 1994 in situ hybridization α-tubulin β-tubulin lacZ fusion gene cilia molluscan development differentiation marker Differential distribution of morphogenetic compounds plays a crucial role in the specification of the early cell fate in molluscan development...