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1-20 of 121
Keywords: in situ hybridization
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Journal Articles
Open-source, high-throughput targeted in situ transcriptomics for developmental and tissue biology
Open AccessHower Lee, Christoffer Mattsson Langseth, Sergio Marco Salas, Sanem Sariyar, Andreas Metousis, Eneritz Rueda-Alaña, Christina Bekiari, Emma Lundberg, Fernando Garcı́a-Moreno, Marco Grillo, Mats Nilsson
Journal:
Development
Development (2024) 151 (16): dev202448.
Published: 29 August 2024
... transcriptomics, readily applicable to a wide range of tissue types and model organisms. Spatial transcriptomics In situ hybridization Multiplex imaging Multi-omics Open source Padlock probes Chan Zuckerberg Initiative Silicon Valley Community Foundation http://dx.doi.org...
Includes: Supplementary data
Journal Articles
Harry M. T. Choi, Colby R. Calvert, Naeem Husain, David Huss, Julius C. Barsi, Benjamin E. Deverman, Ryan C. Hunter, Mihoko Kato, S. Melanie Lee, Anna C. T. Abelin, Adam Z. Rosenthal, Omar S. Akbari, Yuwei Li, Bruce A. Hay, Paul W. Sternberg, Paul H. Patterson, Eric H. Davidson, Sarkis K. Mazmanian, David A. Prober, Matt van de Rijn, Jared R. Leadbetter, Dianne K. Newman, Carol Readhead, Marianne E. Bronner, Barbara Wold, Rusty Lansford, Tatjana Sauka-Spengler, Scott E. Fraser, Niles A. Pierce
Journal:
Development
Development (2016) 143 (19): 3632–3637.
Published: 1 October 2016
...; David A. Prober; Matt van de Rijn; Jared R. Leadbetter; Dianne K. Newman; Carol Readhead; Marianne E. Bronner; Barbara Wold; Rusty Lansford; Tatjana Sauka-Spengler; Scott E. Fraser; Niles A. Pierce In situ hybridization methods are used across the biological sciences to map mRNA expression within intact...
Includes: Supplementary data
Journal Articles
Katsuhiko Ono, Adrien Clavairoly, Tadashi Nomura, Hitoshi Gotoh, Aoi Uno, Olivier Armant, Hirohide Takebayashi, Qi Zhang, Kenji Shimamura, Shigeyoshi Itohara, Carlos M. Parras, Kazuhiro Ikenaka
Journal:
Development
Development (2014) 141 (10): 2075–2084.
Published: 15 May 2014
... is disorganized in the Olig2-KO dorsal thalamus, and initial elongation of TCAs is retarded in the Olig2-KO forebrain. Microarray analysis demonstrated upregulation of several axon guidance molecules, including Epha3 and Epha5 , in the Olig2-KO basal forebrain. In situ hybridization showed that the prethalamus...
Includes: Supplementary data
Journal Articles
DjPum , a homologue of Drosophila Pumilio, is essential to planarian stem cell maintenance
Available to PurchaseAlessandra Salvetti, Leonardo Rossi, Annalisa Lena, Renata Batistoni, Paolo Deri, Giuseppe Rainaldi, Maria Teresa Locci, Monica Evangelista, Vittorio Gremigni
Journal:
Development
Development (2005) 132 (8): 1863–1874.
Published: 15 April 2005
... ) * These authors contributed equally to this work 14 2 2005 ©2005. 2005 Planarians Regeneration Neoblasts Stem cells RNAi Cell proliferation Pumilio PUF proteins Confocal microscopy TEM Cytofluorimetry In situ hybridization Post-transcriptional regulation Stem cells...
Journal Articles
Distinct roles of Rac1/Cdc42 and Rho/Rock for axon outgrowth and nucleokinesis of precerebellar neurons toward netrin 1
Available to PurchaseFrédéric Causeret, Matias Hidalgo-Sanchez, Philippe Fort, Stéphanie Backer, Michel-Robert Popoff, Cécile Gauthier-Rouvière, Evelyne Bloch-Gallego
Journal:
Development
Development (2004) 131 (12): 2841–2852.
Published: 15 June 2004
.... Fig. 6. Expression of Rock in mouse at E11-E13. At E11 (A,A′), Rock1 mRNA could be detected by in situ hybridization in the ventricular (short arrow) and subventricular zone (large arrow) of the rhombic lip. Note the presence of Rock1 -positive cells in the early migratory pathway (arrowheads...
Includes: Multimedia, Supplementary data
Journal Articles
The genetic network of prototypic planarian eye regeneration is Pax6 independent
Available to PurchaseDavid Pineda, Leonardo Rossi, Renata Batistoni, Alessandra Salvetti, Maria Marsal, Vittorio Gremigni, Alessandra Falleni, Javier Gonzalez-Linares, Paolo Deri, Emili Saló
Journal:
Development
Development (2002) 129 (6): 1423–1434.
Published: 15 March 2002
.... The expression pattern of Pax6A and Pax6B was analyzed by whole-mount in situ hybridization in intact adult specimens of both species. The presence of Pax6A transcripts was observed in the cephalic ganglia and in the nerve cords that cross the entire organism ventrally. ( Fig. 3 A-G). Pax6B showed...
Journal Articles
Reconstitution of the organizer is both sufficient and required to re-establish a fully patterned body plan in avian embryos
Available to Purchase
Journal:
Development
Development (1999) 126 (11): 2461–2473.
Published: 1 June 1999
... ). In situ hybridization analysis of chick embryos in whole mount and tissue sections . In Methods in Avian Embryology . Methods in Cell Biology , vol. 51 , (ed. M. Bronner-Fraser ). New York : Academic Press, Inc . Nieuwkoop , P. D. ( 1969 ). he formation of the mesoderm in urodelan...
Journal Articles
Timing and cell interactions underlying neural induction in the chick embryo
Available to Purchase
Journal:
Development
Development (1999) 126 (11): 2505–2514.
Published: 1 June 1999
... Forest, IL 60045, USA 17 03 1999 04 05 1999 © 1999 by Company of Biologists 1999 Blastoderm Epiblast Gastrulation Hensen’s node In situ hybridization Neural plate Neural tube Neurulation Organizer Primitive streak Quail/chick chimera It is conceivable...
Journal Articles
Requirement of a novel gene, Xin , in cardiac morphogenesis
Available to PurchaseDa-Zhi Wang, Rebecca S. Reiter, Jenny Li-Chun Lin, Qin Wang, Haley S. Williams, Sonja L. Krob, Thomas M. Schultheiss, Sylvia Evans, Jim Jung-Ching Lin
Journal:
Development
Development (1999) 126 (6): 1281–1294.
Published: 15 March 1999
... repeats, DNA-binding domains, SH3-binding motifs and nuclear localization signals, are highly conserved. Northern blot analyses detect a single message of 8.9 and 5.8 kilo base (kb) from both cardiac and skeletal muscle of chick and mouse, respectively. In situ hybridization reveals that the cXin gene...
Journal Articles
Energy deprivation and a deficiency in downstream metabolic target genes during the onset of embryonic heart failure in RXRα −/− embryos
Available to PurchasePilar Ruiz-Lozano, Susan M. Smith, Guy Perkins, Steven W. Kubalak, Gerry R. Boss, Henry M. Sucov, Ronald M. Evans, Kenneth R. Chien
Journal:
Development
Development (1998) 125 (3): 533–544.
Published: 1 February 1998
... Biology and Anatomy, Medical University of South Carolina, Charleston, SC 29425, USA † Author for correspondence (e-mail: [email protected] ) 07 11 1997 13 01 1998 © 1998 by Company of Biologists 1998 Retinoids Heart cDNA subtraction In situ hybridization Energy depletion...
Journal Articles
De novo induction of the organizer and formation of the primitive streak in an experimental model of notochord reconstitution in avian embryos
Available to Purchase
Journal:
Development
Development (1998) 125 (2): 201–213.
Published: 15 January 1998
... Wilkinson , D. G. ( 1992 ). In Situ Hybridization. A Practical Approach . Oxford : IRL Press at Oxford University Press . Yamada , T. , Placzek , M. , Tanaka , H. , Dodd , J. and Jessell , T. M. ( 1991 ). Control of cell pattern in the developing nervous system...
Journal Articles
A role for En-2 and other murine homologues of Drosophila segment polarity genes in regulating positional information in the developing cerebellum
Available to Purchase
Journal:
Development
Development (1995) 121 (12): 3935–3945.
Published: 1 December 1995
.../+ crosses. β-actin probe was provided by Janet Rossant. Both the Pax-3 and Pax-6 probes were the same as used for in situ hybridization. The assay was conducted as described by Ausubel et al. (1987) , using 10 µg of total cerebellar RNA and 5×105 cpm of probe RNA in each reaction. Quantitation...
Journal Articles
Blastomere derivation and domains of gene expression in the Spemann Organizer of Xenopus laevis
Available to Purchase
Journal:
Development
Development (1995) 121 (11): 3505–3518.
Published: 1 November 1995
.... Within the early gastrula Organizer, the genes Xbra, goosecoid, noggin and xNR3 are expressed differently in the animal-vegetal and superficial-deep dimensions. In situ hybridization and lineage labelling define distinct regions of the dorsal marginal zone. By the end of gastrulation, dorsal axial...
Journal Articles
Disruption of the mouse RBP-J к gene results in early embryonic death
Available to PurchaseChio Oka, Toru Nakano, Andrew Wakeham, Jose Luis de la Pompa, Chisato Mori, Takashi Sakai, Saeko Okazaki, Masashi Kawaichi, Kohei Shiota, Tak W. Mak, Tasuku Honjo
Journal:
Development
Development (1995) 121 (10): 3291–3301.
Published: 1 October 1995
... for introducing us to the whole-mount in situ hybridization technique. REFERENCES Amakawa , R. , Wu , J. , Ozawa , K. , Matsunami , N. , Hamaguchi , Y. , Matsuda , F. , Kawaichi , M. and Honjo , T. ( 1993 ). Human JК recombination signal binding protein gene (IGKJRB...
Journal Articles
Mammary epithelial cells undergo secretory differentiation in cycling virgins but require pregnancy for the establishment of terminal differentiation
Available to Purchase
Journal:
Development
Development (1995) 121 (7): 2079–2090.
Published: 1 July 1995
... of the mammary secretory phenotype. By in situ hybridization, we have established evidence for asynchrony in milk protein gene expression among alveolar cells showing large variations in the intensity of hybridization among adjacent cells. The asynchrony of maturation of epithelial cells within a given alveolus...
Journal Articles
Coordinate expression of the three zona pellucida genes during mouse oogenesis
Available to Purchase
Journal:
Development
Development (1995) 121 (7): 1947–1956.
Published: 1 July 1995
..., by northern analysis and in situ hybridization with 33 P-labelled antisense probes to each of the three mouse zona mRNAs, we have determined that the expression of each mouse zona gene is restricted to the oocyte. ZP2 transcripts, but not ZP1 or ZP3, are detected in resting (15 μm diameter) oocytes, and all...
Journal Articles
Transforming growth factor α disrupts the normal program of cellular differentiation in the gastric mucosa of transgenic mice
Available to PurchaseRichard Sharp, Mark W. Babyatsky, Hitoshi Takagi, Sven Tågerud, Timothy C. Wang, Dale E. Bockman, Stephen J. Brand, Glenn Merlino
Journal:
Development
Development (1995) 121 (1): 149–161.
Published: 1 January 1995
... and neonatal mammalian stomach. TGFα has been localized by in situ hybridization and by immunohistochemical visualization to the mature, non-proliferative compartment of the gastric mucosa, suggestive of a role in cellular differentiation ( Beauchamp et al., 1989 ; Thomas et al., 1992 ; Nasim et al., 1992...
Journal Articles
Expression of activin subunits, activin receptors and follistatin in postimplantation mouse embryos suggests specific developmental functions for different activins
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Journal:
Development
Development (1994) 120 (12): 3621–3637.
Published: 1 December 1994
...A. Feijen; M. J. Goumans; A. J. M. van den; Eijnden-van Raaij ABSTRACT Using in situ hybridization we have studied the localization of the messenger RNAs encoding the inhibin/activin subunits (α, βA, βB), the activin-binding protein follistatin and activin receptors (IIA, IIB) in mouse embryos...
Journal Articles
Soma-germline asymmetry in the distributions of embryonic RNAs in Caenorhabditis elegans
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Journal:
Development
Development (1994) 120 (10): 2823–2834.
Published: 1 October 1994
...Geraldine Seydoux; Andrew Fire ABSTRACT Early embryogenesis in Caenorhabditis elegans is characterized by a series of unequal cleavages that mark the stepwise separation of somatic and germ lineages. We have developed an in situ hybridization protocol to examine the localization of specific...
Journal Articles
Transcriptional regulation of tubulin gene expression in differentiating trochoblasts during early development of Patella vulgata
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Journal:
Development
Development (1994) 120 (10): 2835–2845.
Published: 1 October 1994
... by Company of Biologists 1994 in situ hybridization α-tubulin β-tubulin lacZ fusion gene cilia molluscan development differentiation marker Differential distribution of morphogenetic compounds plays a crucial role in the specification of the early cell fate in molluscan development...
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