... transcriptomics, readily applicable to a wide range of tissue types and model organisms. Spatial transcriptomics In situ hybridization Multiplex imaging Multi-omics Open source Padlock probes Chan Zuckerberg Initiative Silicon Valley Community Foundation http://dx.doi.org...

...; David A. Prober; Matt van de Rijn; Jared R. Leadbetter; Dianne K. Newman; Carol Readhead; Marianne E. Bronner; Barbara Wold; Rusty Lansford; Tatjana Sauka-Spengler; Scott E. Fraser; Niles A. Pierce In situ hybridization methods are used across the biological sciences to map mRNA expression within intact...

... is disorganized in the Olig2-KO dorsal thalamus, and initial elongation of TCAs is retarded in the Olig2-KO forebrain. Microarray analysis demonstrated upregulation of several axon guidance molecules, including Epha3 and Epha5 , in the Olig2-KO basal forebrain. In situ hybridization showed that the prethalamus...

... ) * These authors contributed equally to this work 14 2 2005 ©2005. 2005 Planarians Regeneration Neoblasts Stem cells RNAi Cell proliferation Pumilio PUF proteins Confocal microscopy TEM Cytofluorimetry In situ hybridization Post-transcriptional regulation Stem cells...

... close to the floor plate, whereas LRN migrate and cross the floor plate ventro-marginally to locate contralaterally to their birthplace. We first delineated the cartography of Rho GTPases expression by in situ hybridization on hindbrain cryosections. ION were identified through their expression...

.... Evolutionary distances were calculated using Kimura’s equation ( Nei and Koehn, 1983 ), and used for phylogenetic tree construction by the Neighbor-joining method. Sequences were obtained from the EMBL/ GenBank. All sense and antisense digoxigenin-labeled RNA probes used for in situ hybridization...

... primitive streak. * Author for correspondence (e-mail: [email protected] ) 18 3 1999 4 5 1999 © 1999 by Company of Biologists 1999 Cardiac mesoderm Chick embryos Gastrulation Gene expression Heart In situ hybridization Neurulation Nieuwkoop center...

... Forest, IL 60045, USA 17 03 1999 04 05 1999 © 1999 by Company of Biologists 1999 Blastoderm Epiblast Gastrulation Hensen’s node In situ hybridization Neural plate Neural tube Neurulation Organizer Primitive streak Quail/chick chimera Three main functions have...

... repeats, DNA-binding domains, SH3-binding motifs and nuclear localization signals, are highly conserved. Northern blot analyses detect a single message of 8.9 and 5.8 kilo base (kb) from both cardiac and skeletal muscle of chick and mouse, respectively. In situ hybridization reveals that the cXin gene...

... Biology and Anatomy, Medical University of South Carolina, Charleston, SC 29425, USA † Author for correspondence (e-mail: [email protected] ) 07 11 1997 13 01 1998 © 1998 by Company of Biologists 1998 Retinoids Heart cDNA subtraction In situ hybridization Energy depletion...

... 22 , 1 – 3 . 10.1073/pnas.22.1.1 Wilkinson , D. G. ( 1992 ). In Situ Hybridization. A Practical Approach . Oxford : IRL Press at Oxford University Press . Yamada , T. , Placzek , M. , Tanaka , H. , Dodd , J. and Jessell , T. M. ( 1991 ). Control of cell...

... ). Total RNA was extracted using the method of Sekiguchi et al. (1992) from postnatal day 5 (P5) cerebella, dissected from littermates of En-2hd/+ × En-2hd/+ crosses. β-actin probe was provided by Janet Rossant. Both the Pax-3 and Pax-6 probes were the same as used for in situ hybridization...

.... Within the early gastrula Organizer, the genes Xbra, goosecoid, noggin and xNR3 are expressed differently in the animal-vegetal and superficial-deep dimensions. In situ hybridization and lineage labelling define distinct regions of the dorsal marginal zone. By the end of gastrulation, dorsal axial...

... somite defect neural tube defect in situ hybridization homologous recombination mouse RBP-Jк is a unique transcription factor that does not contain any of the known DNA-binding protein motifs such as zinc finger, helix turn helix, helix loop helix and leucine zipper ( Matsunami et al., 1989...

..., by northern analysis and in situ hybridization with 33 P-labelled antisense probes to each of the three mouse zona mRNAs, we have determined that the expression of each mouse zona gene is restricted to the oocyte. ZP2 transcripts, but not ZP1 or ZP3, are detected in resting (15 μm diameter) oocytes, and all...

... of the mammary secretory phenotype. By in situ hybridization, we have established evidence for asynchrony in milk protein gene expression among alveolar cells showing large variations in the intensity of hybridization among adjacent cells. The asynchrony of maturation of epithelial cells within a given alveolus...

... Department of Internal Medicine, Gunma University School of Medicine, Maebashi Gunma, 371 Japan 20 09 1994 © 1995 by Company of Biologists 1995 TGFα stomach parietal cell chief cell apoptosis Ménétrier’s disease achlorhydria in situ hybridization mouse The glands...

...A. Feijen; M. J. Goumans; A. J. M. van den; Eijnden-van Raaij ABSTRACT Using in situ hybridization we have studied the localization of the messenger RNAs encoding the inhibin/activin subunits (α, βA, βB), the activin-binding protein follistatin and activin receptors (IIA, IIB) in mouse embryos...

...Geraldine Seydoux; Andrew Fire ABSTRACT Early embryogenesis in Caenorhabditis elegans is characterized by a series of unequal cleavages that mark the stepwise separation of somatic and germ lineages. We have developed an in situ hybridization protocol to examine the localization of specific...

... by Company of Biologists 1994 in situ hybridization α-tubulin β-tubulin lacZ fusion gene cilia molluscan development differentiation marker Differential distribution of morphogenetic compounds plays a crucial role in the specification of the early cell fate in molluscan development...