... large-scale in situ hybridisation screens (wholemount and section) and microarray gene expression data of microdissected, laser-captured and FACS-sorted components of the developing mouse genitourinary (GU) system. These expression data are annotated using a high-resolution anatomy ontology specific...

... Development Mouse Homeodomain protein Msx gene Heterospecific graft β-galactosidase Differentiation In situ hybridisation Although much attention has focused on the muscle and cartilage derivatives during somite differentiation, very little is known about the formation of another major...

... at the brachial level. 6 hours post surgery, Msx1 nlacZ expression was revealed on the operated side by salmon-gal (red) staining and mouse Pax3 expression by in situ hybridisation (blue staining). Note the faint cross-hybridisation of the mouse-specific Pax3 probe with chick transcripts in the chick...

... and parathyroids. This ‘cardiac’ neural crest sub-population is assumed to exist in mammals, but without direct evidence. In this paper we demonstrate, using RT-PCR and in situ hybridisation, that Pax3 expression can serve as a marker of cardiac neural crest cells in the mouse embryo. Cells of this lineage were...

..., respectively, and (2) the preventive effect of maternally administered retinoic acid (5 mg/kg) on spinal neural tube defects in curly tail (ct/ct) mice. Using in situ hybridisation and computerised image analysis we show here that in ct/ct embryos, RAR-β transcripts are deficient in the hindgut endoderm...

... development and thus help control the time of onset and the rate of myelination in peripheral nerves. * Author for correspondence 17 03 1994 © 1994 by Company of Biologists 1994 DNA synthesis in situ hybridisation forskolin differentiation cyclic AMP Schwann cell P o gene...

...-independent immediate-early genes. * Author for correspondence 21 01 1994 © 1994 by Company of Biologists 1994 mesoderm induction cytoplasmic determinants gene expression Xwnt-8 goosecoid Xbra Xenopus axis determination in situ hybridisation In early...

... activity within the embryo, examined using -galactosidase ( -gal) fusions, is similar to that of the endogenous mRNA seen by in situ hybridisation. The contribution of elements within the 5 sequence have been assessed by comparing the expression patterns of constructs that have deletions in this region...

...Marion C. Dickson; Hans G. Slager; Elizabeth Duffie; Christine L. Mummery; Rosemary J. Akhurst ABSTRACT We have performed a detailed analysis of the localisations of RNAs for TGFβ2 and β3, and of TGFβ2 protein in mouse embryos from 6.5 to 9.5 days post coitum, using in situ hybridisation...

.... PrP gene in situ hybridisation mouse embryo Scrapie, Creutzfeldt-Jakob disease and bovine spongiform encephalopathy are transmissible neurodegenerative disorders associated with alterations in the neural membrane protein PrP (Oesch et al., 1985 ; review: Hope and Manson, 1991...

... somite contained detectable l ectin immunoreactivity (Fig. 4 , panels 5 to 7). Fig. 5. In situ hybridisation of 35 S-labelled antisense Lectl4 probe to a longitudinal section of a 10.5 dpc embryo under bright-field (1,3) and dark-field (2,4) illumination. Panels 3 and 4 show detail...

...Maria Sjöberg; Björn Vennström; Douglas Forrest ABSTRACT Thyroid-hormone-dependent development of the neuroretina has principally been described in amphibia. Here, we show by in situ hybridisation that mRNAs coding for three distinct thyroid hormone receptors (TRs), TR α and two TR β variants...

... in situ hybridisation mouse development A number of strategies have been used to identify genes involved in murine embryogenesis. Firstly, the murine homologues of genes known to regulate development in other species, most notably Drosophila, have been cloned and characterised (reviewed...

...Peter Schmid; Andreas Lorenz; Horst Hameister; Mathias Montenarh ABSTRACT By in situ hybridisation we have examined the expression of p53 during mouse embryogenesis from day 8.5 to day 18.5 post coitum (p.c.) . High levels of p53 mRNA were detected in all cells of the day 8.5 p.c . and 10.5 p.c...

... fragment containing the complete coding region of mouse AMH was isolated. In situ hybridisation was used to determine the precise timing and localisation of AMH expression in male and female embryos and postnatal testes and ovaries. AMH transcripts were first detected in fetal testes at 12.5 Ape when...

...Peter Schmid; David Cox; Graeme Bilbe; Rainer Maier; Gary K. Mcmaster ABSTRACT We have examined by Northern analysis and in situ hybridisation the expression of TGF β1 β2and β3 during mouse embryogenesis. TGF β1 is expressed predominantly in the mesodermal components of the embryo e.g...

... expression in situ hybridisation mitosis Pisum sativum storage protein The pattern of growth of legume seeds follows a sigmoidal curve, beginning with a phase of cell division, where there is little increase in absolute mass, followed by a phase of cell expansion (Pate and Flinn, 1971...

... beta3 but apparently with lower transcript prevalence. A comparison of relative tran script levels of different genes by in situ hybridisation is difficult to make, despite control over the size and specific activity of the radioactive probe. However, one conclusion that can be drawn regarding...

... of Biologists 1990 mouse embryo homeobox in situ hybridisation The homeobox was originally identified as a 180 bp nucleotide sequence found at the 3’ ends of many genes involved in the formation of the body plan during Drosophila development (reviewed by Gehring, 1987). The 180 bp...

... to the proposition that the biological effects of TGF beta-1 may, in part, be mediated by Jl/tenascin. In our previous in situ hybridisation study of TGF beta1 RNA distribution within the midgestation murine embryo, a full-length cDNA probe against TGF beta-1 was employed (Lehnert and Akhurst, 1988...