... acquired from the unfertilised egg during embryogenesis through to the adult drone thorax and loss during spermatogenesis. Intriguingly, this epigenetic reprogramming pattern is the same as that observed for a number of CpGs at imprinted genes in the paternal germ line of mammals ( Ferguson-Smith et al...

...Charlotte E. Rutledge; Avinash Thakur; Karla M. O’Neill; Rachelle E. Irwin; Shun Sato; Ken Hata; Colum P. Walsh A functional role for DNA methylation has been well-established at imprinted loci, which inherit methylation uniparentally, most commonly from the mother via the oocyte. Many CpG islands...

...Thiet Minh Vu; Miyuki Nakamura; Joseph P. Calarco; Daichi Susaki; Pei Qi Lim; Tetsu Kinoshita; Tetsuya Higashiyama; Robert A. Martienssen; Frédéric Berger In mammals and plants, parental genomic imprinting restricts the expression of specific loci to one parental allele. Imprinting in mammals...

... silences the paternal allele of AtFH5 , restricting its expression to the maternal allele. AtFH5 thus appears to be a new, maternally expressed imprinted gene. We further demonstrate that AtFH5 is responsible for morphological defects caused by the loss of PcG activity in the seed. † Author...

... (ncRNA)Kcnq1ot1 regulates epigenetic gene silencing in an imprinted gene cluster in cis over a distance of 400 kb in the mouse embryo, whereas the silenced region extends over 780 kb in the placenta. Gene silencing by the Kcnq1ot1 RNA involves repressive histone modifications, including H3K9me2...

... whether the parent-of-origin-dependent seed abortion caused by lack of the FIS subunit MSI1 is caused by parental imprinting of the MSI1 gene. Our data show that MSI1 is not an imprinted gene and that early paternal MSI1 expression is not sufficient to rescue msi1 mutant seeds. By contrast, expression...

...Annabelle Lewis; Kelly Green; Claire Dawson; Lisa Redrup; Khanh D. Huynh; Jeannie T. Lee; Myriam Hemberger; Wolf Reik The mouse Kcnq1 imprinted domain is located on distal chromosome 7 and contains several imprinted genes that are paternally repressed. Repression of these genes is regulated...

..., parental-specific imprinting marks are maintained to direct appropriate imprinted gene expression. We previously demonstrated that H19 imprinting could be lost during preimplantation development under certain culture conditions. To define the lability of genomic imprints during this dynamic period...

...Piroska E. Szabó; Shih-Huey E. Tang; Michael R. Reed; Francisco J. Silva; Walter M. K. Tsark; Jeffrey R. Mann Imprinting of the mouse insulin-like growth factor 2 ( Igf2 ) and H19 genes is regulated by an imprinting control region (ICR). The hypomethylated maternal copy functions as a chromatin...

...Pantelis Georgiades; Marie Watkins; M. Azim Surani; Anne C. Ferguson-Smith ABSTRACT Genetic analysis has shown that the distal portion of mouse chromosome 12 is imprinted; however, the developmental roles of imprinted genes in this region are not known. We have therefore generated conceptuses...

... mechanism that controls monoallelic gene expression in this domain. * Author for correspondence (email: [email protected] ) 22 08 2000 10 07 2000 © 2000 by Company of Biologists 2000 Igf2 H19 Imprinting Silencer YAC transgene Tongue Skeletal muscle...

... and female cells. In the mouse, the paternally derived X chromosome (X P ) is imprinted and preferentially inactivated in the extraembryonic tissues whereas in the embryonic tissues inactivation is random. To investigate how X P is chosen as an inactivated X chromosome in the extraembryonic cells, we have...

..., erasure of allele-specific methylation associated with imprinted genes, and the re-activation of the X chromosome. The allele-specific differential methylation is involved in regulating the monoallelic expression, and thus the gene dosage, of imprinted genes, which underlies functional differences between...

... a pronounced overgrowth of rib cartilage. Excess concentrations of IGFII, a potent mitogen, has been suggested to play a major role in these defects, as andro-genetic cells possess two active paternal copies of the imprinted Igf2 gene, rather than one inactive maternal and one active paternal copy as in normal...

... (e-mail: [email protected] ) 23 09 1996 © 1996 by Company of Biologists 1996 chromosome elimination sister chromatid separation sex determination imprinting Sciara Bradysia The accurate replication and segregation of chromosomes are defining features...

...K. John McLaughlin; Piroska Szabó; Hélène Haegel; Jeffrey R. Mann ABSTRACT Imprinted genomic regions have been defined by the production of mice with uniparental inheritance or duplication of homologous chromosome regions. With most of the genome investigated, paternal duplication of only distal...

...Hiroyuki Sasaki; Anne C. Ferguson-Smith; Alisa S. W. Shum; Sheila C. Barton; M. Azim Surani ABSTRACT The mouse H19 gene is imprinted so that the paternal copy is both methylated and repressed during fetal development. However, the CpG-rich promoter region encompassing the transcription start...

...Yasuyuki Kimura; R. Yanagimachi ABSTRACT Genomic imprinting occurs in both male and female gametes during gametogenesis, but the exact time when imprinting begins and ends is unknown. In the present study we injected nuclei of testicular spermatozoa and round spermatids into mature mouse oocytes...

... to IGF2 , is monoallelically expressed in all postnatal liver samples analysed. We conclude that the functional imprinting status of IGF2 during postnatal liver development appears to be promoter/enhancer-specific and either partly or completely independent of H19 . 11 11 1994 © 1995...

... of preferential paternal imprinting during days 1 to 9 of in vitro differentiation of ES cells. However, the influence of the Xce locus on the randomness of X-inactivation in embyros seems to operate also in ES cell lines. Our overall conclusion is that the low levels of Xist RNA in female kidney, embryos...