... in whole-mount vertebrate embryos and brain sections. RNA fluorescence in situ hybridization RNA-FISH Immunofluorescence qHCR imaging dHCR imaging Whole-mount zebrafish embryos Mouse brain sections National Institute of Biomedical Imaging and Bioengineering http://dx.doi.org...

... and localization can be confirmed using appropriate strategies, including mouse genetics and, if possible, by confirming protein localization by immunofluorescence. Second, guide RNA (gRNA) design should follow best practices so as to minimize the chance of on/off-target indel alleles; targeting protein C-termini...

... to retrorsine. At various time points, liver tissue was harvested and cells in DPPIV+ colonies were phenotyped by immunofluorescence and histochemical protocols. Functional differentiation and liver replacement were determined by comparing donor and host hepatocyte protein expression patterns and DPPIV enzyme...

.... A,C,E: anti myc IF; B,D,F: 13F4 IF. Bar, 100 µ m. (A,B) E2·5: the myc signal (A) is very bright in myotomal cell nuclei (arrowheads). m, myotome; d, dermatome: s, sclerotome. 13F4 immunofluorescence delineates myotomal cells (B). (C,D) E3 chick embryo. Myc expression is maximal in myotomal cells...

... differentiation, whereas about 100 ventral ectoderm cells were necessary for epidermal differentiation. 28 09 1988 © 1989 by Company of Biologists 1989 Xenopus embryo monoclonal antibody immunofluorescence microculture dissociated cells The aim of the present study was to develop...

...Mark Page The distribution of cytokeratins and vimentin intermediate filaments in the first 48 h of chick development has been determined using immunofluorescent labelling. During formation of the germ layers, cytokeratin expression is associated with the appearance of an integral epithelium...

...Evelyn Houliston; Marie-Noëlle Guilly; Jean-Claude Courvalin; Bernard Maro The expression of nuclear lamins during mouse preimplantation development was studied by immunofluorescence, immunoblotting and immunoprecipitation. Two sera were used, specific either for lamin B or lamins A and C. Both...

... embryogenesis. maternal-effect genes homeobox protein gradient immunofluorescence segmentation genes Drosophila The anteroposterior body pattern of a developed Drosophila embryo consists of a defined number and sequence of segments. The formation of these metameric subdivisions depends...

... that the critical step for initiation of migration of NC cells may be the loss of adhesions between cells. 29 06 1987 © 1987 by Company of Biologists 1987 basal lamina neural crest chick embryos mouse embryos collagen IV laminin fibronectin immunofluorescence immunogold Various...