...Daisy J. Vinter; Caroline Hoppe; Thomas G. Minchington; Catherine Sutcliffe; Hilary L. Ashe ABSTRACT The Hunchback (Hb) transcription factor is crucial for anterior-posterior patterning of the Drosophila embryo. The maternal hb mRNA acts as a paradigm for translational regulation due to its...

... of birth. We show that the U1-U5 neurons project axons sequentially, followed by sequential dendrite extension. We misexpressed the earliest temporal transcription factor, Hunchback, to create ‘ectopic’ U1 neurons with an early intrinsic temporal identity but later birth-order. These ectopic U1 neurons...

... Crustacean cardioactive peptide (CCAP) . There are two types of CCAP neurons: interneurons and efferent neurons. We found that both are specified during the Hunchback temporal window of neuroblast 3-5, but are not sibling cells. Further, this temporal window generates two ganglion mother cells that give rise...

...Khoa D. Tran; Michael R. Miller; Chris Q. Doe The Hunchback/Ikaros family of zinc-finger transcription factors is essential for specifying the anterior/posterior body axis in insects, the fate of early-born pioneer neurons in Drosophila , and for retinal and immune development in mammals. Hunchback...

...Khoa D. Tran; Chris Q. Doe Neurogenesis in Drosophila and mammals requires the precise integration of spatial and temporal cues. In Drosophila , embryonic neural progenitors (neuroblasts) sequentially express the transcription factors Hunchback, Kruppel, Pdm1/Pdm2 (Pdm) and Castor as they generate...

... is specified by the transient expression of the transcription factor Hunchback. When reaching the next temporal identity, this expression is switched off in the neuroblasts by seven up ( svp ) in a mitosis-dependent manner, but is maintained in their progeny (ganglion mother cells). We show that svp mRNA...

... products to direct axial patterning than do Drosophila embryos. In Drosophila , anterior axial patterning is largely established by bicoid , a rapidly evolving maternal-effect gene, working with hunchback , which is expressed both maternally and zygotically. Here,we focus on a comparative analysis...

... germ insect, Gryllus bimaculatus ( Gb ). Here, we have focused on its hunchback ortholog( Gb ' hb ), because Drosophila hb functions as a gap gene during anterior segmentation, referred as a canonical function. Gb'hb is expressed in a gap pattern during the early stages of embryogenesis, and later...

... is required for activation of the gap genes hunchback and Krüppel . Given the large RNAi deletion phenotype and its regulation of hunchback and Krüppel, even-skipped seems to act as an über-gap gene in Oncopeltus , indicating that it may have both upstream and downstream roles in segmentation. We further...

... of the homolog of the Drosophila gap gene hunchback in an intermediate germ insect, the milkweed bug, Oncopeltus fasciatus . We find that Oncopeltus hunchback ( Of'hb ) is expressed in two phases,first in a gap-like domain in the blastoderm and later in the posterior growth zone during germband elongation...

... Hunchback (Hb) is expressed in the early sublineage of NB7-3, which consists of the early NB and the first GMC (GMC7-3a) and its progeny (EW1 and GW), prompted us to investigate its possible role in NB7-3 lineage development. Our analysis revealed that loss of hb results in a lack of the normally Hb...

.... The expression of these marker genes suggests that embryonic pattern formation in the grasshopper occurs as cells move together to form the blastodisc. ‡Author for correspondence (e-mail: [email protected] ) 8 6 2001 © 2001. 2001 Locust Wingless Decapentaplegic Caudal Hunchback...

...-rule genes such as even-skipped are not conserved between these species. This might suggest that the factors upstream of pair-rule gene expression are not conserved across insect species. We find that, despite this, many aspects of the expression of the Drosophila gap gene hunchback are shared with its...

... characterised Bicoid target gene, hunchback , at the anterior pole when its expression should be repressed by Torso. Persistence of hunchback at the pole mimics most of the torso phenotype and leads to repression at early stages of a labral ( cap’n’collar ) and two foregut ( wingless and hedgehog ) determinants...

... mutant embryos have anterior and posterior gap defects, resembling loss of both maternal and zygotic Drosophila hunchback function; squiggy mutant embryos develop only four full trunk segments, a phenotype more severe than those caused by lack of Drosophila maternal or zygotic terminal gene functions...

... addition during insect evolution. We addressed this question by analysing the regulation of potential target genes of bicoid in the short germ embryo of Tribolium castaneum . We demonstrate that homologues of caudal and hunchback from Tribolium are regulated by Drosophila bicoid . In Drosophila , maternal...

...Christopher Wreden; Arturo C. Verrotti; Jennifer A. Schisa; Marshal E. Lieberfarb; Sidney Strickland ABSTRACT Nanos protein promotes abdominal structures in Drosophila embryos by repressing the translation of maternal hunchback mRNA in the posterior. To study the mechanism of nanos -mediated...

..., is distributed in an anterior-to-posterior gradient in the embryo. In response to this gradient, the zygotic gene hunchback is expressed uniformly in the anterior half of the embryo in a nearly all- or-none manner. In this report we demonstrate that a recombinant Bicoid protein binds cooperatively to its sites...

...Jonathan S. Margolis; Mark L. Borowsky; EirÍkur SteingrÍmsson; Chung Wha Shim; Judith A. Lengyel; James W. Posakony ABSTRACT The gap gene hunchback ( hb ) is required for the formation and segmentation of two regions of the Drosophila embryo, a broad anterior domain and a narrow posterior domain...

... regulatory effects on their target genes. However, there is so far little direct in vivo evidence that a single factor can autonomously activate and repress a given target gene. We have analysed here the regulatory capabilities of the gap gene hunchback by creating an artificial gradient of hunchback...