...Wenyi Yang; Mingjian Peng; Youquan Wang; Xiaowen Zhang; Wei Li; Xue Zhai; Zhichao Wu; Peng Hu; Liangbiao Chen Iron is essential for cell growth and hematopoiesis, regulated by hepcidin ( hamp ). However, hamp 's role in zebrafish hematopoiesis remains unclear. Here, we created a stable hamp...

... that does not derive from HSC and displays reduced dependence on flt3 compared to the conventional HSC-derived dendritic cells. Zebrafish Hematopoiesis Dendritic cell Flt3 National Key Research and Development Program of China 2023YFA1800100 Shenzhen Medical Research Fund...

... with unequal amounts of lysosomes and of the differentiation marker CD34. Such asymmetric inheritance generates heterogeneity in the progeny, which is likely to contribute to the plasticity of the early steps of hematopoiesis. Mitotic cell images were resliced to visualize the centrosomes and the point...

... specification ( Baranasic et al., 2022 ; Prasch et al., 2006 ; Reischauer et al., 2016 ; Tanguay et al., 2000 ). Many genes involved in embryonic hematopoiesis and vascular development are evolutionally conserved between zebrafish and mammals like mice and humans ( Begley et al., 1989 ; Casie Chetty et...

...Xue Zhang; Yuxi Yang; Yuxuan Wei; Qingshun Zhao; Xin Lou ABSTRACT Hematopoiesis is a highly coordinated process that generates all the body's blood cells, and perturbations in embryonic hematopoiesis may result in illnesses ranging from fetal anemia to various leukemias. Correct establishment...

... lineages and is involved in hematopoietic stem/progenitor cell and myeloid cell development in zebrafish. Zebrafish Hematopoiesis Hematopoietic stem/progenitor cells ETS transcription factors National Key Research and Development Program of China 2018YFA0800200 Shenzhen Bay...

... to hypoxia. As the maturation of myeloid and lymphoid cells depends on Csf1rb, but erythroid cell maturation does not rely on Csf1rb, the compensatory hematopoiesis compromises erythroid but not myeloid and lymphoid defects over time. For 11 dpf juvenile fish, fish were anesthetized and killed on ice...

... as important determinants of organ homeostasis, tissue repair, remodeling and regeneration. Although the ontogeny and function of tissue-resident macrophages has been identified as distinct from postnatal hematopoiesis, the inability to specify, in vitro , similar populations that recapitulate...

...Donn A. Van Deren; Shrutokirti De; Ben Xu; Kayla M. Eschenbacher; Shuhua Zhang; Mario R. Capecchi ABSTRACT Previously, we have demonstrated that a subpopulation of microglia, known as Hoxb8 microglia, is derived from the Hoxb8 lineage during the second wave (E8.5) of yolk sac hematopoiesis, whereas...

... functional roles in TLC development during steady-state hematopoiesis, although IL7Rα appears to be more important than Flk2, as indicated by the more severe reduction in cell numbers. To test whether TLCs arise via differentiation pathways similar to circulating lymphocytes, we examined labeling...

... differentiation protocols. We wanted to determine whether this decrease in HSPC emergence and colonization of the CHT indicated a sustained reduction in definitive hematopoiesis. We assessed rag1 expression in the 5 dpf thymus, as thymic seeding is dependent on the emergence of definitive HSPCs from the DA...

... in hematopoiesis . Semin. Cell Dev. Biol. 14 , 143 - 150 . 10.1016/S1084-9521(02)00183-0 Pal , M. , Ghosh , S. and Bose , I. ( 2014 ). Non-genetic heterogeneity, criticality and cell differentiation . Phys. Biol. 12 , 016001 . 10.1088/1478-3975/12/1/016001 Rackauckas...

... blood cell types in Drosophila and act as signaling centers to neighboring cells. Drosophila Intermediate progenitor Blood cell development Crystal cells Hematopoiesis Split GAL4 National Institutes of Health R01 HL-067395 R01 CA-217608 T32HL863458 K12GM106996 T32HL69766...

... The authors declare no competing or financial interests. 24 9 2020 12 3 2021 © 2021. Published by The Company of Biologists Ltd 2021 Summary: This study investigates the process of early T cell development, and how adult hematopoiesis is regulated by distinct mechanisms...

... microglial waves, we uncover non-overlapping roles for csf1ra and csf1rb in hematopoiesis, and identified csf1rb as an essential regulator of adult microglia development. Notably, we demonstrate that csf1rb positively regulates HSC-derived myelopoiesis, resulting in macrophage deficiency, including microglia...

... taxa. Here, we demonstrate novel PSEDN roles in vivo in Drosophila hematopoiesis and in human erythropoiesis in vitro . Using Drosophila genetics, we show that PSEDN members function with GATA to block lamellocyte differentiation and maintain the prohemocyte pool. Overexpression of human SIX1...

..., hematopoiesis and blood vessel formation . EMBO J.   25 , 3955 - 3965 . 10.1038/sj.emboj.7601273 Bae , S. C. , Ogawa , E. , Maruyama , M. , Oka , H. , Satake , M. , Shigesada , K. , Jenkins , N. A. , Gilbert , D. J. , Copeland , N. G. and Ito , Y. ( 1994...

...Rohan J. Khadilkar; Guy Tanentzapf ABSTRACT Hematopoiesis requires coordinated cell signals to control the proliferation and differentiation of progenitor cells. In Drosophila , blood progenitors, called prohemocytes, which are located in a hematopoietic organ called the lymph gland, are regulated...

... in non-hemogenic endothelial cells promotes blood cell formation in ectopic sites in the mouse conceptus, but only if Runx1 is expressed before embryonic day 8.5. Hematopoiesis Runx1 Hemogenic endothelium Embryo National Heart, Lung and Blood Institute R01HL091724 U01HL100405...

...Chen U. Zhang; Ken M. Cadigan The lymph gland (LG) is a major source of hematopoiesis during Drosophila development. In this tissue, prohemocytes differentiate into multiple lineages, including macrophage-like plasmatocytes, which comprise the vast majority of mature hemocytes. Previous studies...