...Nicole E. Franks; Benjamin L. Allen ABSTRACT Growth arrest specific 1 (GAS1) is a key regulator of mammalian embryogenesis, best known for its role in hedgehog (HH) signaling, but with additional described roles in the FGF, RET, and NOTCH pathways. Previous work indicated a later role for GAS1...

.... To enhance this model, we developed an immortalized lizard fibroblast cell line (ASEC-1) for the exploration of reptilian gene function in cellular processes. We demonstrate the use of this cell line by scrutinizing the role of primary cilia in lizard Hedgehog (Hh) signaling. Using CRISPR/Cas9 mutagenesis...

...Sinclair W. Emans; Armen Yerevanian; Fasih M. Ahsan; Jen F. Rotti; Yifei Zhou; Lucydalila Cedillo; Alexander A. Soukas ABSTRACT Both hedgehog (Hh) and target of rapamycin complex 2 (TORC2) are central, evolutionarily conserved signaling pathways that regulate development and metabolism. In C...

...Nicole Roberto; Isabelle Becam; Anne Plessis; Robert A. Holmgren ABSTRACT Morphogen gradients need to be robust, but may also need to be tailored for specific tissues. Often this type of regulation is carried out by negative regulators and negative feedback loops. In the Hedgehog (Hh) pathway...

... the activation of the EGFR effector, ERK. Also, we found that loss of ben , but not the JNK kinase hemipterous , resulted in an upregulation of hedgehog signaling, increased proliferation and increased niche competition. Lastly, we demonstrate that the hypercompetition phenotype caused by loss of ben...

...Hsiao-Fan Lo; Mingi Hong; Henrietta Szutorisz; Yasmin L. Hurd; Robert S. Krauss ABSTRACT Many developmental disorders are thought to arise from an interaction between genetic and environmental risk factors. The Hedgehog (HH) signaling pathway regulates myriad developmental processes, and pathway...

...Emily K. Ho; Tim Stearns ABSTRACT The mechanisms of vertebrate Hedgehog signaling are linked to the biology of the primary cilium, an antenna-like organelle that projects from the surface of most vertebrate cell types. Although the advantages of restricting signal transduction to cilia are often...

...Tanvi Gore; Tamás Matusek; Gisela D'Angelo; Cécile Giordano; Thomas Tognacci; Laurence Lavenant-Staccini; Catherine Rabouille; Pascal P. Thérond ABSTRACT The Hedgehog (Hh) morphogen gradient is required for patterning during metazoan development, yet the mechanisms involved in Hh apical...

...Martha L. Echevarría-Andino; Benjamin L. Allen ABSTRACT The Hedgehog (HH) pathway controls multiple aspects of craniofacial development. HH ligands signal through the canonical receptor PTCH1, and three co-receptors: GAS1, CDON and BOC. Together, these co-receptors are required during embryogenesis...

...Ryo Hatori; Thomas B. Kornberg ABSTRACT Hedgehog (Hh) is an evolutionarily conserved signaling protein that has essential roles in animal development and homeostasis. We investigated Hh signaling in the region of the Drosophila wing imaginal disc that produces Hh and is near the tracheal air sac...

... in adaxial slow precursors, thereby initiating trunk myogenesis. After gastrulation, when similar muscle cell populations in the post-anal tail are generated from tailbud, declining Fgf signalling is less effective at initiating adaxial myogenesis, which is instead initiated by Hedgehog signalling from...

... work is properly attributed. Summary: This Review summarizes the spatial and temporal gene regulatory patterning systems that produce the diversity and organization of neurons in the vertebrate spinal cord. Gene regulatory network Hedgehog Neural tube Neurogenesis Neuronal diversity...

... photoreceptors that travels at constant speed throughout the retinal epithelium. Drosophila Hedgehog Patterning Systems modelling Visual system Morphogens of the hedgehog ( hh )/Shh family contribute spatial information during the development of a wide range of organs and organisms ( Ingham...

...Cecile Giordano; Laurent Ruel; Candice Poux; Pascal Therond ABSTRACT Hedgehog (Hh) is a conserved morphogen that controls cell differentiation and tissue patterning in metazoans. In Drosophila , the Hh signal is transduced from the G protein-coupled receptor Smoothened (Smo) to the cytoplasmic Hh...

... signaling interactions between the stroma and nephron progenitor compartment have been proposed to control nephron development. Here, we show that removal of hedgehog intracellular effector smoothened ( Smo -deficient mutants) in the cortical stroma results in an abnormal renal capsule, and an expanded...

...Chengbing Wang; Jia Li; Ken-Ichi Takemaru; Xiaogang Jiang; Guoqiang Xu; Baolin Wang ABSTRACT The primary cilium is a microtubule-based organelle required for Hedgehog (Hh) signaling and consists of a basal body, a ciliary axoneme and a compartment between the first two structures, called...

...Sun-hee Hwang; Kevin A. White; Bandarigoda N. Somatilaka; John M. Shelton; James A. Richardson; Saikat Mukhopadhyay ABSTRACT The role of basal suppression of the sonic hedgehog (Shh) pathway and its interaction with Indian hedgehog (Ihh) signaling during limb/skeletal morphogenesis is not well...

...Guangwei Hu; Guang Li; Hui Wang; Yiquan Wang Correct patterning of left-right (LR) asymmetry is essential during the embryonic development of bilaterians. Hedgehog (Hh) signaling is known to play a role in LR asymmetry development of mouse, chicken and sea urchin embryos by regulating Nodal...

... Hedgehog-containing cytonemes and identified a role for the Hedgehog deployment protein Dispatched in cytoneme stabilization. We demonstrate that Hedgehog and Dispatched colocalize in cytonemes, and that cholesterol-modified Hedgehog acts through Dispatched to increase cytoneme occurrence. Live imaging...

...Weitao Chen; Hai Huang; Ryo Hatori; Thomas B. Kornberg Morphogen concentration gradients that extend across developmental fields form by dispersion from source cells. In the Drosophila wing disc, Hedgehog (Hh) produced by posterior compartment cells distributes in a concentration gradient...