...-type embryos were heat-shocked at the 10-somite stage, and fixed 1 h later. ChIP for 2000 embryos per replicate, using 10 µg goat anti-Myc tag antibody (Abcam, ab9132), was carried out as in Wardle et al. (2006) , with the exception that purified immunoprecipitated DNA was directly prepared...

.... (e-mail: [email protected]) 28 07 1997 © 1997 by Company of Biologists 1997 Caenorhabditis elegans pattern formation vab-7 lp-1 apx-1 skn-1 pie-1 mex-3 pal-1 heat shock The study of numerous maternal effect genes in the C. elegans embryo has...

... 1994 © 1994 by Company of Biologists 1994 argos Drosophila eye development secreted protein determination heat shock The interaction between cells plays an important part in the determination of cell fate. These interactions range from the communication between abutting...

...Acaimo González-Reyes; Ana Macías; Ginés Morata ABSTRACT Previously published experiments have shown that the endogenous Dfd gene can be ectopically activated by its own (heat-shock-driven) product in a subset of cells of different segments. This results in the differentiation of maxillary...

...Nick Ovsenek; John J. Heikkila ABSTRACT We have examined the activity of the Xenopus heat-shock transcription factor (HSF) in extracts from stressed and unstressed embryos at various stages of development using DNA mobility shift analysis. A specific interaction between HSF and a synthetic...

..., heat shock resulted in at least a 100-fold increase in CAT activity levels in late blastula and gastrula stage embryos injected with either construct. In contrast to blastula and gastrula stage embryos, neurula stage embryos that were transformed with Xenopus hsp 70/CAT exhibited a constitutive level...

...Rashmi Kothary; Susan Clapoff; Susan Darling; Marc D. Perry; Laurence A. Moran; Janet Rossant ABSTRACT Transgenic mice have been generated that express the E. coli β-galactosidase gene under the control of the promoter from the mouse heat-shock gene, hsp68 . Sequences from -664 to +113 relative...

...Robin E. Davis; Mary Lou King ABSTRACT We have investigated the Xenopus heat-shock response of somatic cells, oocytes and embryos. Xenopus defolli-culated oocytes displayed a highly variable response to heat shock depending on the culture medium. Intact follicles, however, respond to heat stress...

...D. R. N. Primmett; C. D. Stern; R. J. Keynes ABSTRACT A single heat shock, given to 2-day-old chick embryos, can generate multiple but discrete somite and skeletal anomalies. Each of these anomalies is restricted to one, or at the most two, consecutive segments. The anomalies are separated from...

...Jean-Claude Beetschen; Jean Gautier ABSTRACT Axolotl eggs were heat shocked (36·8°C, 10min) inside their jelly layers. Heat shock (HS) was shown to induce the precocious appearance of a grey crescent (GC) in a number of eggs immediately after fertilization (Benford & Namenwirth, 1974...

... of heat-shock protein (HSP-70) gene expression. * Current address: The Proctor and Gamble Company, Miami Valley Laboratories, PO Box 39175, Cincinnati, OH 45247, USA 11 03 1987 © 1987 by Company of Biologists 1987 metallothionein rabbit blastocyst metal ions heat shock...

... uniformity indicates good timekeeping. Timekeeping is not impaired among the survivors following heat shocks that retard development and disturb and curtail morphogenesis. The immediate effect of heat shock is a stoppage of development, the duration of which is the same for all embryos in the same treatment...

...Jane E. Mee; Vernon French ABSTRACT A heat shock (of 15 min at 48°C) given to early embryos of the locust, Schistocerca gregaria , results in localized abnormalities in the segment pattern subsequently formed. Most defects involve two consecutive segments of the thorax or abdomen...

...Jane E. Mee; Vernon French ABSTRACT The effect of heat shock (15 min at 48 °C) on segmentation has been investigated in the short germ embryo of the locust (Schistocerca gregaria) . Prior to formation of the germ anlage and at the disc stage heat shock considerably reduced the survival of eggs...