... of lateral vessel progenitors (LVPs). These phenotypes contrast with the increased IM and absent LVPs observed with loss of the bHLH transcription factor Hand2, and loss of hand2 partially suppresses osr1 mutant phenotypes. hand2 and osr1 are expressed together in the posterior mesoderm, but osr1 expression...

... remain challenging. Here, using multiple knockout mouse models and single cell RNA sequencing, we demonstrate that the divergent lncRNA Hand2os1 / Uph has a key complex modulatory effect on the expression of its neighboring gene HAND2 and subsequently on heart development and function. Short deletion...

... systemically explored. Here, we revealed a gradient of HAND2 protein in cardiac progenitors in the anterior dorsal pericardial wall (aDPW) and adjacent transition zone (TZ) in the outflow tract (OFT). Deletion of Hand2 caused cell arrest and accumulation in the TZ, leading to defective morphogenesis. Although...

...-Notch Dlx5a Hand2 Emx2 Fsta Mrrf National Institute of Dental and Craniofacial Research 10.13039/100000072 DE018405, K99DE026239 National Institute of Neurological Disorders and Stroke 10.13039/100000065 R00NS080913 A.P. Giannini Foundation 10.13039/100002112...

... cardiomyocytes. The basic helix-loop-helix (bHLH) transcription factor Hand2 has been implicated in promoting cardiomyocyte formation. It is unclear, however, whether Hand2 plays an instructive or permissive role during this process. Here, we find that overexpression of hand2 in the early zebrafish embryo...

... alx4a activated expression in fibroblasts and osteoblasts within anterior fin rays, whereas hand2 regulatory sequences activated expression in these same cell types within posterior rays. Transgenic overexpression of hand2 in all pectoral fin rays did not affect formation of the proliferative...

... that ectopic joints develop at the expense of cartilage. Time-lapse experiments suggest that barx1 controls the skeletal precursor cell choice between differentiating into cartilage versus joint cells. We discovered that barx1 functions in this choice, in part, by regulating the transcription factor hand2. We...

...Junji Itou; Hiroko Kawakami; Thu Quach; Marco Osterwalder; Sylvia M. Evans; Rolf Zeller; Yasuhiko Kawakami How divergent genetic systems regulate a common pathway during the development of two serial structures, forelimbs and hindlimbs, is not well understood. Specifically, HAND2 has been shown...

...Jun Lei; Marthe J. Howard Targeted deletion of the bHLH DNA-binding protein Hand2 in the neural crest, impacts development of the enteric nervous system (ENS), possibly by regulating the transition from neural precursor cell to neuron. We tested this hypothesis by targeting Hand2 deletion in nestin...

...Zayra V. Garavito-Aguilar; Heather E. Riley; Deborah Yelon Heart formation requires the fusion of bilateral cardiomyocyte populations as they move towards the embryonic midline. The bHLH transcription factor Hand2 is essential for cardiac fusion; however, the effector genes that execute...

...Jared Coffin Talbot; Stephen L. Johnson; Charles B. Kimmel The ventrally expressed secreted polypeptide endothelin1 (Edn1) patterns the skeleton derived from the first two pharyngeal arches into dorsal, intermediate and ventral domains. Edn1 activates expression of many genes, including hand2...

...Ning Liu; Ana C. Barbosa; Shelby L. Chapman; Svetlana Bezprozvannaya; Xiaoxia Qi; James A. Richardson; Hiromi Yanagisawa; Eric N. Olson The basic helix-loop-helix (bHLH) transcription factor Hand2 is required for growth and development of the heart, branchial arches and limb buds. To determine...

...Marsha E. Lucas; Frank Müller; Roland Rüdiger; Paul D. Henion; Hermann Rohrer The basic helix-loop-helix transcription factor Hand2 , together with Ascl1, Phox2a, Phox2b and Gata2/Gata3 , is induced by bone morphogenetic proteins in neural crest-derived precursor cells during sympathetic neuron...

...David G. McFadden; Ana C. Barbosa; James A. Richardson; Michael D. Schneider; Deepak Srivastava; Eric N. Olson The basic helix-loop-helix transcription factors Hand1 and Hand2 display dynamic and spatially restricted expression patterns in the developing heart. Mice that lack Hand2 die at embryonic...

... and joints in the anterior pharyngeal arches of young larvae. Here we present genetic analyses in the zebrafish of two edn1 downstream targets, the bHLH transcription factor Hand2 and the homeobox transcription factor Bapx1, that mediate dorsoventral (DV) patterning in the anterior pharyngeal arches. First...

... that the basic helix-loop-helix (bHLH) transcription factor dHAND/Hand2 (Srivastava et al., 1995), also known as Thing-2 / Hed (Hollenberg et al., 1995 ; Cross et al., 1995), is expressed in a remarkably dynamic expression pattern throughout limb bud outgrowth and differentiation. At the onset of limb...