... of genetic and pharmacological manipulations in zebrafish to show that during neurogenesis FGF activity occurs as a gradient along the anterior-posterior axis of the dorsal midbrain and directs spatially dynamic expression of the Hairy gene her5 . As FGF activity diminishes during development, Her5 is lost...

... levels, nuclear migration, apical cell constriction and an acceleration of the furrow. We find that these roles are distinct from the bHLH protein Hairy (H), which we show restricts atonal ( ato ) expression ahead of the furrow. Secondary furrow initiation along the dorsal and ventral margins is blocked...

.... * Author for correspondence ( [email protected] ) Competing interests statement The authors declare no competing financial interests. 21 8 2012 © 2012. 2012 Chick Cath1 Atoh1 Atonal 1 Gdf7 Cyp26c1 Hes Hairy Transthyretin Co-culture Electroporation GFP...

... that the zebrafish hairy - and enhancer of split -related (Her) genes her3 and her9 are expressed in the inter-proneuronal domains, and are required for their formation. her3 and her9 expression was not regulated by Notch signaling, but rather controlled by positional cues, in which Bmp signaling is involved...

... growth and the generation of midbrain-hindbrain neurons. Recently, we implicated the Hairy/E(spl) transcription factor Her5 in the formation of the medial (most basal) part of the IZ (MIZ) in zebrafish; the molecular bases for lateral IZ (LIZ) formation, however, remain unknown. We now demonstrate...

..., a pattern that depends on periodic expression of the hairy gene ( h ) and the proneural genes achaete ( ac ) and scute ( sc ). Expression of ac in longitudinal stripes in prepupal leg discs defines the positions of the mechanosensory bristle rows. The ac / sc expression domains are delimited by the Hairy...

... factor in the spatial definition of proneural domains in the zebrafish neural plate, in a manner similar to its Drosophila homologue Hairy. † Author for correspondence (e-mail: [email protected] ) * Present address: Institute of Toxicology and Genetics, Forschungszentrum Karlsruhe, PO 3640, D-76021...

... the same cDNA library under less stringent conditions (50°C). We thus isolated an additional Hairy homologue that we denominated Amphi-hairyE. Amphi-hairyA to Amphi-hairyD plus an additional Amphi-hairyF genes were also identified through an EST project of gastrula and neurula stages( Panopoulou et...

... expression of genes defining MHB identity. We further show that expression of the zebrafish bHLH Hairy/E(spl)-related factor Her5 prefigures and then precisely delineates the IZ throughout embryonic development. Using morpholino knock-down and conditional gain-of-function assays, we demonstrate that Her5...

... Chick Segmentation clock Segmentation Cyclic gene expression hairy lunatic fringe In the vertebrate body, segments are conspicuous at the level of the vertebral column, the associated muscles and peripheral nervous system. This segmental pattern is laid down during embryogenesis through...

... in these cells. Mutation of ref-1 also affects the fate of other epidermal cells in distinct AP body regions. ref-1 encodes a protein with two basic helix-loop-helix domains distantly related to those of the hairy/Enhancer of split family. ref-1 , and another hairy homolog, lin-22 , regulate similar cell fate...

... and specify diverse cell types by creating unique local environments which establish the expression of locally acting genes. This process is exemplified by the patterning of leg microchaete rows. hairy ( h ) is expressed in a spatially restricted manner in the leg imaginal disc and functions to position adult...

... lineages rather than V5-like lineages. lin-22 activity is also required to prevent additional neurons from being produced by one branch of the V5 lineage. We find that the lin-22 gene exhibits homology to the Drosophila gene hairy , and that lin-22 activity represses neural development within the V5...

...Chihcheng Tsai; Peter Gergen ABSTRACT The segmentation genes runt and hairy are required for the proper transcriptional regulation of the pair-rule gene fushi tarazu during the blastoderm stage of Drosophila embryogenesis. The expression of different fushi tarazu reporter genes was examined in runt...

...James A. Langeland; Sherwin F. Attai; Kathy Vorwerk; Sean B. Carroll ABSTRACT We present a genetic and molecular analysis of two hairy ( h ) pair-rule stripes in order to determine how gradients of gap proteins position adjacent stripes of gene expression in the posterior of Drosophila embryos. We...

...Michael Lardelli; David Ish-Horowicz ABSTRACT The hairy ( h ) segmentation gene of Drosophila regulates segmental patterning of the early embryo, and is expressed in a set of anteroposterior stripes during the blastoderm stage. We have used a set of h gene deletions to study the h promoter...

... is generated. For example, the legs of the Drosophila melanogaster adult are covered with bristles, which in most segments are arranged in longitudinal rows. Here we elucidate the key roles of two regulatory genes, hairy and achaete , in setting up this periodic bristle pattern. We show that achaete...

...James A. Langeland; Sean B. Carroll ABSTRACT The hairy (h) gene is one of two pair-rule loci whose striped expression is directly regulated by combinations of gap proteins acting through discrete upstream regulatory fragments, which span several kilobases. We have undertaken a comparative study...

...Nadean L. Brown; Carol A. Sattler; Donald R. Markey; Sean B. Carroll ABSTRACT The regulatory gene hairy is expressed and required during early embryogenesis to control segmentation gene expression properly and during larval and pupal development to control the pattern of certain adult sensory...

...-rule gene hairy . The eve and hairy expression patterns overlap but are out of register and the cells of maximal overlap form the anterior margin of the polarized eve stripe. We propose that the relative placement of the eve and hairy stripes may be an important factor in the initiation of segment...