... gene knockdown experiments and validations of the identified interactions by cis-regulatory analysis. We use multi-omics involving next-generation sequencing to obtain the necessary information for drafting the Strongylocentrotus purpuratus (Sp) posterior gut GRN. Here, we present an update to the GRN...

...Yi-Ning Kang; Candice Fung; Pieter Vanden Berghe ABSTRACT During embryonic development, the gut is innervated by intrinsic (enteric) and extrinsic nerves. Focusing on mammalian ENS development, in this Review we highlight how important the different compartments of this innervation are to assure...

... it remains controversial whether AChE functions in this capacity in vivo . Here, we show that AChE plays an essential non-classical role in vertebrate gut morphogenesis. Exposure of Xenopus embryos to AChE-inhibiting chemicals results in severe defects in intestinal development. Tissue-targeted loss...

... rotation of the intestine is directed by forces outside the gut, but the morphogenetic events that generate anatomical asymmetry in other regions of the digestive tract remain unknown. Here, we show in mouse and Xenopus that the mechanisms that drive the curvature of the stomach are intrinsic to the gut...

... gene expression in the embryonic zebrafish brain . Neural Dev. 6 , 10 . 10.1186/1749-8104-6-10 Lengyel , J. A. and Iwaki , D. D. ( 2002 ). It takes guts: the Drosophila hindgut as a model system for organogenesis . Dev. Biol. 243 , 1 - 19 . 10.1006/dbio.2002.0577 Li...

... role of JNK in this context has remained elusive. Here, we show that JNK activity is indispensable for the rearrangement of endoderm cells that underlies the elongation of the Xenopus gut tube. Whereas Rho kinase is necessary to induce cell intercalation and remodel adhesive contacts, we have found...

... of the somatic gonad, the gut and other non-gonadal tissues, as well as in sperm, but expression is not observed in oocytes. Results of tissue-specific egrh-1(RNAi) experiments and genetic mosaic analyses revealed that EGRH-1 function is necessary in the soma and, surprisingly, this function is required in both...

... is essential for cell shape changes and morphogenesis in the developing vertebrate gut and that Shroom3 transcription in the gut requires the Pitx1 transcription factor. Moreover, we show that Pitx proteins directly activate Shroom3 transcription, and we identify Pitx-responsive regulatory elements...

...Cammon B. Arrington; H. Joseph Yost One of the first steps in zebrafish heart and gut organogenesis is the migration of bilateral primordia to the midline to form cardiac and gut tubes. The mechanisms that regulate this process are poorly understood. Here we show that the proteoglycan syndecan 2...

...) as an HSC inducing signal. * Author for correspondence (e-mail: [email protected] ) 21 5 2009 © 2009. 2009 AGM Hematopoiesis Hedgehog Gut Mouse embryo Positional information Patterning of the vertebrate embryo occurs through the complex interactions between...

... the number of NCC by ablating the neural tube adjacent to somites 3-6 to produce aganglionic gut. We then back-transplanted various somite lengths of quail neural tube into the ablated region to determine the `tipping point',whereby sufficient progenitors were available for complete ENS formation...

... transition (EMT), and subsequently differentiate into endothelial and vascular smooth muscle cells. This is thought to be a unique process in blood vessel formation. Still,structural and developmental similarities between the heart and gut led us to test the hypothesis that a conserved or related mechanism...

..., revealed GATA5 and 6 as the most critical for the development of the gut and the liver. In this study we explore the roles of the transcription factors, GATA4, 5 and 6, in the formation of endoderm and its derivatives. Although all three factors are involved, GATA6 appears to be a key direct activator...

...Hsiu-Ting Tseng; Rina Shah; Milan Jamrich Development of the visceral mesoderm is a critical process in the organogenesis of the gut. Elucidation of function and regulation of genes involved in the development of visceral mesoderm is therefore essential for an understanding of gut organogenesis...

...Alan J. Burns; Jean-Marie M. Delalande; Nicole M. Le Douarin The enteric nervous system (ENS) is derived from vagal and sacral neural crest cells (NCC). Within the embryonic avian gut, vagal NCC migrate in a rostrocaudal direction to form the majority of neurons and glia along the entire length...

... malformations occur. The phenotype is specific to HFGa13 expression in the posterior endoderm, and includes taillessness and severe gut/genitourinary (GGU) malformations. Finally, we show that chick HFGa13 negatively regulates expression of Hoxd13 and antagonizes functions of both endogenous Hoxa13 and Hoxd13...

... . This is usually attributed to the fact that no reversals of stable left-right asymmetries have been observed in Drosophila , although relevant surveys have been carried out. We have focused on the asymmetry of the proventriculus in the embryonic gut of Drosophila , an aspect of left-right asymmetry...

...Devyn M. Smith; Corinne Nielsen; Clifford J. Tabin; Drucilla J. Roberts ABSTRACT Patterning of the gut into morphologically distinct regions results from the appropriate factors being expressed in strict spatial and temporal patterns to assign cells their fates in development. Often, the boundaries...

...Andrew D. Chalmers; Jonathan M. W. Slack ABSTRACT We have produced a comprehensive fate map showing where the organs of the gut and respiratory system are derived from in the early Xenopus laevis endoderm. We also show the origin of the associated smooth muscle layer on a separate fate map...

...Oliver Pabst; Robert Zweigerdt; Hans-Henning Arnold ABSTRACT The homeodomain transcription factor Nkx2-3 is expressed in gut mesenchyme and spleen of embryonic and adult mice. Targeted inactivation of the Nkx2-3 gene results in severe morphological alterations of both organs and early postnatal...