... of target genes and the steps leading to de novo establishment of PcG-mediated repression. We describe a genetic system in which a Drosophila PcG target gene, giant ( gt ), is ubiquitously repressed during early embryogenesis by a maternally expressed transcription factor, and show the temporal recruitment...

.... In Drosophila , embryos mutant for giant show a gap in the anterior as well fusions of several abdominal segments. In Tribolium , a short germ beetle, giant is required for segmental identity, but not formation, in gnathal segments and also for segmentation of the entire abdomen. This raises the possibility...

... Krüppel Giant Even-skipped Dfd Scr Antp Ubx Short germ Long germ segmentation Gap gene Abdomen Jaws Tribolium castaneum Drosophila Evolution Parental RNAi Anteroposterior patterning in Drosophila is controlled by regulatory elements that measure the local...

... that formation of these segments may rely on a different mechanism. Nevertheless, the expression and function of many segmentation genes seem conserved between Tribolium and Drosophila . We have cloned the Tribolium ortholog of the abdominal gap gene giant . As in Drosophila , Tribolium giant is expressed in two...

...Xuelin Wu; Rajesh Vakani; Stephen Small ABSTRACT We have combined genetic experiments and a targeted misexpression approach to examine the role of the gap gene giant ( gt ) in patterning anterior regions of the Drosophila embryo. Our results suggest that gt functions in the repression of three...

... ); these work synergistically to activate the stripe in the anterior half of the embryo. bcd also coordinates the expression of two repressors, giant (gt) and Kruppel (Kr), which define the anterior and posterior borders of the stripe, respectively. Here, we report the findings of extensive cis- and trans...

...Rachel Kraut; Michael Levine We describe the regulated expression of the segmen-tation gene giant (gt) during early embryogenesis. The gt protein is expressed in two broad gradients in precellu-lar embryos, one in anterior regions and the other in posterior regions. Double immunolocalization...

... that the segmentation gene giant is a bona fide gap gene that is likely to act in concert with hunchback, Krüppel and knirps to initiate stripes of gene expression. We show that Krüppel and giant are expressed in complementary, non-overlapping sets of cells in the early embryo. These complementary patterns depend...