... cells produce cysts that partially fragment prior to meiosis . Development 140 , 2075 - 2081 . 10.1242/dev.093864 Lei , L. and Spradling , A. C. ( 2016 ). Mouse oocytes differentiate through organelle enrichment from sister cyst germ cells . Science 352 , 95 - 99...

.... SUMOylation UBC9 Fertility Germ cell National Institutes of Health R01HD085994 R35GM136340 T32HD098068 R21HD109807 DK114356 T32HD098068 In mammals, oocyte development begins embryonically but is not completed until the oocyte is fertilized following ovulation in adults. Prior...

... zebrafish primordial germ cells that migrate throughout the developing embryo. When migrating within an ectodermal environment, the germ cells form fewer and smaller blebs when compared with their behavior within mesodermal environment. We find that cortical tension of neighboring cells is a parameter...

...Chun-Wei Feng; Guillaume Burnet; Cassy M. Spiller; Fiona Ka Man Cheung; Kallayanee Chawengsaksophak; Peter Koopman; Josephine Bowles ABSTRACT In mice, the entry of germ cells into meiosis crucially depends on the expression of stimulated by retinoic acid gene 8 ( Stra8 ). Stra8 is expressed...

... concentrations of S1P. These effects can be rescued by addition of the 12-Lox product 12-S-HETE. Blocking ABC transporter, PLA2 or 12-Lox activity also inhibits homing of germ cells in vivo . Using a live-imaging chemotaxis assay in a 3D matrix, we show that a shallow gradient of 12-S-HETE enhances chemotaxis...

.../user-licence-1-1/ Summary: Conditional deletion of the E2 SUMO-conjugating enzyme in mouse oocytes causes female infertility as a result of compound defects in ovarian follicle development and oocyte meiotic maturation and progression. Reproduction Fertility Germ cell Transcription...

... is involved in a variety of cellular processes, including ribosome biogenesis, cellular differentiation, germ cell development and tumorigenesis. However, the mechanisms by which prmt5 influences cellular processes have remained unclear. Here, prmt5 loss in zebrafish led to the expression of an infertile male...

...Kazuko Hanyu-Nakamura; Kazuki Matsuda; Stephen M. Cohen; Akira Nakamura ABSTRACT Specification of germ cells is pivotal to ensure continuation of animal species. In many animal embryos, germ cell specification depends on maternally supplied determinants in the germ plasm. Drosophila polar granule...

...Philip D. Campbell; Amanda E. Heim; Mordechai Z. Smith; Florence L. Marlow In animals, specification of the primordial germ cells (PGCs), the stem cells of the germ line, is required to transmit genetic information from one generation to the next. Bucky ball (Buc) is essential for germ plasm (GP...

... in culture. More recent work has shown that, in vivo , mammalian germ cells maintain poised chromatin at promoters of many genes that regulate somatic development, and that they retain this state from fetal stages through meiosis and gametogenesis. We hypothesize that the poised chromatin state is essential...

... In vertebrates that have been examined to date, the sexual identity of germ cells is determined by the sex of gonadal somatic cells. In the teleost fish medaka, a sex-determination gene on the Y chromosome, DMY / dmrt1bY , is expressed in gonadal somatic cells and regulates the sexual identity of germ cells...

...Lei Lei; Allan C. Spradling Mammalian germ cells divide mitotically and form nests of associated cells just prior to entering meiosis. At least some nests contain germline cysts that arise by synchronous, incomplete mitotic divisions, but others may form by aggregation. To systematically...

...Cassy M. Spiller; Chun-Wei Feng; Andrew Jackson; Ad J. M. Gillis; Antoine D. Rolland; Leendert H. J. Looijenga; Peter Koopman; Josephine Bowles Germ cells, the embryonic precursors of sperm or oocytes, respond to molecular cues that regulate their sex-specific development in the fetal gonads...

... a well-established role in regulating the survival and migration of germ cells. We now show that wun has an essential tissue-autonomous role in development of the trachea: the catalytic activity of Wun is required to maintain septate junction (SJ) paracellular barrier function, loss of which causes...

...Jason D. Heaney; Ericka L. Anderson; Megan V. Michelson; Jennifer L. Zechel; Patricia A. Conrad; David C. Page; Joseph H. Nadeau Testicular teratomas result from anomalies in germ cell development during embryogenesis. In the 129 family of inbred strains of mice, teratomas initiate around embryonic...

... probes of identical length and identical staining duration to allow direct expression level comparison at late gastrula stage (A) and at the onset of somitogenesis (B). ( C ) During early somitogenesis stages, the primordial germ cells (PGCs, brown) clearly reside outside of regions of high cxcl12b...

...Maki Shirae-Kurabayashi; Kazuki Matsuda; Akira Nakamura In many animal embryos, germ-cell formation depends on maternal factors located in the germ plasm. To ensure the development of germ cells, germline progenitors must be prevented from differentiating inappropriately into somatic cells...

.... Strikingly, the first wave of spermatogenesis was totally compromised, as seminiferous tubules of homozygous mutant animals were devoid of post-meiotic germ cells. We determined that spermatogenesis was arrested around the late pachytene-diplotene stages of prophase I; surprisingly, without any obvious...

...Matthew S. Cook; Steven C. Munger; Joseph H. Nadeau; Blanche Capel Human germ cell tumors show a strong sensitivity to genetic background similar to Dnd1 Ter/Ter mutant mice, where testicular teratomas arise only on the 129/SvJ genetic background. The introduction of the Bax mutation onto mixed...

...Andrew D. Renault; Prabhat S. Kunwar; Ruth Lehmann In Drosophila , germ cell survival and directionality of migration are controlled by two lipid phosphate phosphatases (LPP), wunen ( wun ) and wunen-2 ( wun2 ). wun wun2 double mutant analysis reveals that the two genes, hereafter collectively...