... in the Drosophila testis. Germline stem cell Maintenance Differentiation Chromatin Histone modification Epigenetic Signaling pathway Gene expression National Institutes of Health http://dx.doi.org/10.13039/100000002 5T32GM007231 R01HD102474 R35GM127075 F31GM134641 National...

... promoter motif in tinman is crucial for developmental gene regulatory networks in vivo , and for adult heart function and viability. RNA Polymerase II transcription Core promoter Gene expression Nascent transcription Drosophila Heart development German-Israeli Foundation...

... trophoblasts . Sci. Rep. 8 , 5142 . 10.1038/s41598-018-23379-2 Bahar Halpern , K. , Tanami , S. , Landen , S. , Chapal , M. , Szlak , L. , Hutzler , A. , Nizhberg , A. and Itzkovitz , S. ( 2015 ). Bursty gene expression in the intact mammalian liver . Mol...

...Laura J. Harrison; Daniel Bose ABSTRACT Enhancers confer precise spatiotemporal patterns of gene expression in response to developmental and environmental stimuli. Over the last decade, the transcription of enhancer RNAs (eRNAs) – nascent RNAs transcribed from active enhancers – has emerged...

... expression remains unclear. Depletion of molecular factors (e.g. CTCF) underlying topologically associating domains (TADs) leads to modest alterations in gene expression, whereas genomic rearrangements involving TAD boundaries disrupt normal gene expression and can lead to pathological phenotypes. Here, we...

... the low-sugar-induced increase in body size are not fully shared between the sexes, highlighting the importance of including males and females in larval studies even when similar phenotypic outcomes are observed. Fig. 4. A low-sugar diet has sex-biased effects on metabolic gene expression...

... cells rapidly diversify from each other, and investigation of the corresponding gene expression dynamics can help elucidate underlying cellular differentiation programs. However, current plant embryonic transcriptome datasets either lack cell-specific information or have RNA contamination from...

... and concomitantly undergo stronger downregulation by knockdown of either the intracellular Ca 2+ release channel IP 3 R or Set2. IP 3 /Ca 2+ signals thus regulate gene expression through Set2-mediated H3K36 marks on select neuronal genes for the larval to pupal transition. * Present address: Department...

...Ian J. Begeman; Kwangdeok Shin; Daniel Osorio-Méndez; Andrew Kurth; Nutishia Lee; Trevor J. Chamberlain; Francisco J. Pelegri; Junsu Kang ABSTRACT Heart regeneration in regeneration-competent organisms can be accomplished through the remodeling of gene expression in response to cardiac injury...

... by The Company of Biologists Ltd 2020 Summary: This Spotlight gives an overview of the discovery and current understanding of transcriptional adaptation, and discusses how its main features appear to be conserved across species. Transcriptional adaptation Gene expression Genetic compensation...

... Notch signaling and coordinates a global transcriptional switch to promote placental vascular maturation. Its deficiency may underlie human placental disease. G9a Ehmt2 Vascular maturation Placental vascular development Intrauterine growth restriction Epigenetic regulators Gene expression...

...Anna Reade; Laura B. Motta-Mena; Kevin H. Gardner; Didier Y. Stainier; Orion D. Weiner; Stephanie Woo ABSTRACT Here, we describe an optogenetic gene expression system optimized for use in zebrafish. This system overcomes the limitations of current inducible expression systems by enabling robust...

...Swetansu K. Hota; Benoit G. Bruneau Precise gene expression ensures proper stem and progenitor cell differentiation, lineage commitment and organogenesis during mammalian development. ATP-dependent chromatin-remodeling complexes utilize the energy from ATP hydrolysis to reorganize chromatin...

... of genes along the apical-basal and dorsal-ventral axes in the globular embryo, which tended to fluctuate in later developmental stages. We also analyzed gene expression patterns in the early globular embryo and how this relates to expression in embryonic organs at later stages. We confirmed the accuracy...

...L. Carine Stapel; Benoit Lombardot; Coleman Broaddus; Dagmar Kainmueller; Florian Jug; Eugene W. Myers; Nadine L. Vastenhouw Analysis of differential gene expression is crucial for the study of cell fate and behavior during embryonic development. However, automated methods for the sensitive...

... and Newcastle, UK, was established to provide embryonic and fetal material for a variety of human studies ranging from single gene expression analysis to large-scale genomic/transcriptomic studies. Increasingly, HDBR material is enabling the derivation of stem cell lines and contributing towards developments...

... gene expression during mesoderm induction. We find that Brg1 co-localizes with H3K27ac at distal enhancers and is required for robust H3K27 acetylation at distal enhancers that are activated during mesoderm induction. Brg1 is also required to maintain Polycomb-mediated repression of non-mesodermal...

...Ahmet Ay; Jack Holland; Adriana Sperlea; Gnanapackiam Sheela Devakanmalai; Stephan Knierer; Sebastian Sangervasi; Angel Stevenson; Ertuğrul M. Özbudak The vertebrate segmentation clock is a gene expression oscillator controlling rhythmic segmentation of the vertebral column during embryonic...

...). Ninety-six gene custom TaqMan Low-Density Array (TLDA; Life Technologies) cards were used for validation of gene expression. The TaqMan probe and primer sets were selected from predesigned TaqMan gene expression assays (Life Technologies). Three independent RNA isolates for each genotype or developmental...

... the atlas-builder software to benefit a large proportion of the developmental biology community, and the eNeuro atlas to serve as a publicly accessible hub for integrating neuronal attributes – cell lineage, gene expression patterns, axon/dendrite projections, neurotransmitters – and linking them...