... cell-based embryo models Implantation Gastrulation References Ai , Z. , Niu , B. , Yin , Y. , Xiang , L. , Shi , G. , Duan , K. , Wang , S. , Hu , Y. , Zhang , C. , Zhang , C. et al. ( 2023 ). Dissecting peri-implantation development using...

..., distribution and reproduction in any medium provided that the original work is properly attributed. Nodal signaling Optogenetics Zebrafish Morphogen Mesendodermal patterning Gastrulation Eunice Kennedy Shriver National Institute of Child Health and Human Development http://dx.doi.org...

... Extra-embryonic tissues Organizer Gastrulation Primitive streak Amniote embryos Chick Wellcome Trust http://dx.doi.org/10.13039/100010269 107055/Z/15/Z National Research Foundation of Korea http://dx.doi.org/10.13039/501100003725 Ministry of Education http...

... and maintain intervertebral discs. The mechanisms regulating NotoPs emergence are unclear. This knowledge gap persists due to the inherent complexity of cell fate patterning during gastrulation, particularly within the anterior primitive streak (APS), where NotoPs first arise alongside neuro-mesoderm...

...Alyssa A. Emig; Megan Hansen; Sandra Grimm; Cristian Coarfa; Nathan D. Lord; Margot Kossmann Williams Anteroposterior (AP) elongation of the vertebrate body plan is driven by convergence and extension (C&E) gastrulation movements in both the mesoderm and neuroectoderm, but how or whether...

... ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Summary: Examination of Drosophila mutants in which basal membranes fail to form reveals a role for viscous forces in epithelial folding. Gastrulation...

... morphogenesis is not well understood. In this study, we define the transcriptional consequences of TBXT dosage reduction during early human gastrulation using human induced pluripotent stem cell models of gastrulation and mesoderm differentiation. Multi-omic single-nucleus RNA and single-nucleus ATAC sequencing...

...Alexandra Schauer; Kornelija Pranjic-Ferscha; Robert Hauschild; Carl-Philipp Heisenberg ABSTRACT Embryogenesis results from the coordinated activities of different signaling pathways controlling cell fate specification and morphogenesis. In vertebrate gastrulation, both Nodal and BMP signaling play...

...) specification are incompletely understood owing, in part, to limitations in distinguishing CPCs from non-cardiac mesoderm in early gastrulation. We leveraged detection of early cardiac lineage transgenes within a granular single-cell transcriptomic time course of mouse embryos to identify emerging CPCs...

...Guillermo Serrano Nájera; Cornelis J. Weijer ABSTRACT During gastrulation, early embryos specify and reorganise the topology of their germ layers. Surprisingly, this fundamental and early process does not appear to be rigidly constrained by evolutionary pressures; instead, the morphology...

...Lenin Chandran; Wilko Backer; Raphael Schleutker; Deqing Kong; Seyed A. H. Beati; Stefan Luschnig; H.-Arno J. Müller ABSTRACT Src kinases are important regulators of cell adhesion. Here, we have explored the function of Src42A in junction remodelling during Drosophila gastrulation. Src42A...

... enrichment and polarisation at parasegmental boundaries (PSBs) in early Drosophila embryos, from the start of germband extension at gastrulation and throughout the germband extended stages (stages 6 to 11). First, we find that Tartan is required during germband extension for actomyosin enrichment at PSBs...

...Isabell Niedermoser; Tatiana Lebedeva; Grigory Genikhovich ABSTRACT Canonical Wnt (cWnt) signalling is involved in a plethora of basic developmental processes such as endomesoderm specification, gastrulation and patterning the main body axis. To activate the signal, Wnt ligands form complexes...

... derivatives ( Loh et al., 2014 ; Singh et al., 2012 ; Sumi et al., 2008 ), including human organizer tissue ( Martyn et al., 2018 ). National Institutes of Health R35 GM139654 R01 GM101653 Activin/nodal signaling Eomesodermin Wnt signaling Gastrulation Human embryonic stem cells...

... oscillations linked to the segmentation clock are synchronized across cells in the presomitic mesoderm (PSM) and result in tissue-level wave patterns. To examine their onset during mouse embryo development, we studied the dynamics of the segmentation clock gene Lfng during gastrulation. To this end, we...

... the primitive streak, the site of gastrulation. The more external extra-embryonic region (area opaca) was thought to have only nutritive and support functions. Using experimental embryology approaches, this study reveals three separable functions for this outer region. First, juxtaposition of the area opaca...

..., the chick embryo before gastrulation, cells may interpret gradients of positional signals relative to their neighbours to position the primitive streak, establishing bilateral symmetry. Morphogen Chick embryo Polarity Primitive streak Gastrulation Cell communication SMAD Wellcome Trust...

... humans and farm ungulates. The developmental events occurring following hatching in ungulates include the proliferation and maturation of extra-embryonic membranes – trophoblast and hypoblast – and the formation of a flat embryonic disc, similar to that found in humans, which initiates gastrulation prior...

... ), for expression of shRNA during oogenesis. The following optogenetic lines were a gift from the De Renzis lab (EMBL, Heidelberg, Germany): (1) w[*]; UASp-CIBN-pmGFP/CyO; Sb/TM3, (2) UASp-pmCIBN/FM6; Sb/TM6B, and (3) UASp-CRY2-OCRL/TM3. To examine gastrulation defects in embryos with maternal knockdown...

...Yuki Naitou; Go Nagamatsu; Nobuhiko Hamazaki; Kenjiro Shirane; Masafumi Hayashi; Makoto Hayashi; Satoru Kobayashi; Katsuhiko Hayashi ABSTRACT In mammals, primordial germ cells (PGCs), the origin of the germ line, are specified from the epiblast at the posterior region where gastrulation...