...) specification are incompletely understood owing, in part, to limitations in distinguishing CPCs from non-cardiac mesoderm in early gastrulation. We leveraged detection of early cardiac lineage transgenes within a granular single-cell transcriptomic time course of mouse embryos to identify emerging CPCs...

...Guillermo Serrano Nájera; Cornelis J. Weijer ABSTRACT During gastrulation, early embryos specify and reorganise the topology of their germ layers. Surprisingly, this fundamental and early process does not appear to be rigidly constrained by evolutionary pressures; instead, the morphology...

...Lenin Chandran; Wilko Backer; Raphael Schleutker; Deqing Kong; Seyed A. H. Beati; Stefan Luschnig; H.-Arno J. Müller ABSTRACT Src kinases are important regulators of cell adhesion. Here, we have explored the function of Src42A in junction remodelling during Drosophila gastrulation. Src42A...

... enrichment and polarisation at parasegmental boundaries (PSBs) in early Drosophila embryos, from the start of germband extension at gastrulation and throughout the germband extended stages (stages 6 to 11). First, we find that Tartan is required during germband extension for actomyosin enrichment at PSBs...

...Isabell Niedermoser; Tatiana Lebedeva; Grigory Genikhovich ABSTRACT Canonical Wnt (cWnt) signalling is involved in a plethora of basic developmental processes such as endomesoderm specification, gastrulation and patterning the main body axis. To activate the signal, Wnt ligands form complexes...

... in the differentiation of human embryonic stem cells into mesendoderm. Activin/nodal signaling Eomesodermin Wnt signaling Gastrulation Human embryonic stem cells Mesendodermal differentiation National Institutes of Health R35 GM139654 R01 GM101653 Understanding the transition from...

... oscillations linked to the segmentation clock are synchronized across cells in the presomitic mesoderm (PSM) and result in tissue-level wave patterns. To examine their onset during mouse embryo development, we studied the dynamics of the segmentation clock gene Lfng during gastrulation. To this end, we...

... the primitive streak, the site of gastrulation. The more external extra-embryonic region (area opaca) was thought to have only nutritive and support functions. Using experimental embryology approaches, this study reveals three separable functions for this outer region. First, juxtaposition of the area opaca...

... provided that the original work is properly attributed. Highlighted Article: In a large developing system, the chick embryo before gastrulation, cells may interpret gradients of positional signals relative to their neighbours to position the primitive streak, establishing bilateral symmetry...

... humans and farm ungulates. The developmental events occurring following hatching in ungulates include the proliferation and maturation of extra-embryonic membranes – trophoblast and hypoblast – and the formation of a flat embryonic disc, similar to that found in humans, which initiates gastrulation prior...

... domains during epithelial folding and a role for Dlg1 in coordination of this process. Apical constriction Apical-basal polarity Dlg1 Epithelial folding Gastrulation Ventral furrow National Institute of General Medical Sciences http://dx.doi.org/10.13039/100000057 R35GM128745...

...Yuki Naitou; Go Nagamatsu; Nobuhiko Hamazaki; Kenjiro Shirane; Masafumi Hayashi; Makoto Hayashi; Satoru Kobayashi; Katsuhiko Hayashi ABSTRACT In mammals, primordial germ cells (PGCs), the origin of the germ line, are specified from the epiblast at the posterior region where gastrulation...

... the role of the Dorsal protein nuclear gradient along the dorsoventral axis in defining the graded pattern of actomyosin constriction that initiates gastrulation in early Drosophila embryos. Two complementary mechanisms for graded accumulation of mRNAs of crucial zygotic Dorsal target genes were identified...

... sequences of 2 h-old live Drosophila embryos expressing Sqh::eGFP every 90 s (80 s for simultaneous imaging of tiling and conventional modes and 10 s interval time) for 90 min during cellularization and the beginning of gastrulation ( Fig. 3 ). During this early developmental stage, actomyosin forms...

... proliferation are tightly controlled during gastrulation. * Author for correspondence ( stefano.ditalia@duke.edu ) Competing interests The authors declare no competing or financial interests. © 2021. Published by The Company of Biologists Ltd 2021 Summary: This Review discusses...

... development, as well as in morphogenetic movements of gastrulation and axial elongation. Our findings suggest that the mesoderm-inducing role of FGF extends beyond the group of deuterostomes. The role of FGF signaling in mesoderm induction was thought to be restricted to members of deuterostomes. After...

...Martina Nagel; Debanjan Barua; Erich W. Damm; Jubin Kashef; Ralf Hofmann; Alexey Ershov; Angelica Cecilia; Julian Moosmann; Tilo Baumbach; Rudolf Winklbauer ABSTRACT During Xenopus gastrulation, leading edge mesendoderm (LEM) advances animally as a wedge-shaped cell mass over the vegetally moving...

... and regenerative medicine. Axis elongation Gastrulation Neuromesodermal progenitors Primitive streak Tail bud Pluripotent stem cells Lineage specification in the early vertebrate embryo is initiated during gastrulation (see Glossary, Box 1 ), which involves the formation of the three germ...

... fate labeling and imaging approaches with single-cell RNA sequencing (scRNA-seq) to follow the transcriptional identities and define lineage trajectories of Eomes -dependent cell types. Accordingly, all cells moving through the PS during the first day of gastrulation express Eomes . AM and DE...

...Ulla-Maj Fiuza; Takefumi Negishi; Alice Rouan; Hitoyoshi Yasuo; Patrick Lemaire ABSTRACT Gastrulation is the first major morphogenetic event during animal embryogenesis. Ascidian gastrulation starts with the invagination of 10 endodermal precursor cells between the 64- and late 112-cell stages...