... that the novel functions of the Hox3 homologs zen and bicoid were adopted somewhere in the crustacean-insect clade. In the centipede, the expression of the gene fushi tarazu suggests that it has both a Hox-like role (as in the mite), as well as a role in segmentation (as in insects). This suggests...

... of target genes bound and regulated is a small or large percentage of genes in the genome. In this study, we have addressed these issues using a chimeric protein that contains the strong activation domain of the viral VP16 protein fused to the Drosophila homeodomain-containing protein Fushi tarazu (Ftz). We...

..., the homeodomain-containing transcription factor Fushi tarazu (Ftz), and analyze the response kinetics of known and putative target genes. This is achieved by providing a brief pulse of Ftz expression and measuring the time required for genes to respond. The time required for Ftz to bind and regulate its own...

... of these and other segmentation genes. The results are complex, indicating that Odd is capable of repressing some genes wherever and whenever Odd is expressed, while the ability to repress others is temporally or spatially restricted. Moreover, one target gene, fushi tarazu , is both repressed and activated by Odd...

...Brian Florence; Antoine Guichet; Anne Ephrussi; Allen Laughon ABSTRACT The fushi tarazu pair-rule gene is required for the formation of alternating parasegmental boundaries in the Drosophila embryo. fushi tarazu encodes a homeodomain protein necessary for transcription of the engrailed gene in even...

...Sheryl T. Smith; James B. Jaynes ABSTRACT The engrailed homeoprotein is a dominantly acting or ‘active’ transcriptional repressor both in cultured cells and in vivo. When retargeted via a homeodomain swap to the endogenous fushi tarazu gene ( ftz ), it actively represses it, resulting in a ftz...

...Aleyamma John; Sheryl T. Smith; James B. Jaynes ABSTRACT The Engrailed homeodomain protein is an ‘active’ or dominant transcriptional repressor in cultured cells. In contrast, the Fushi Tarazu homeodomain protein is an activator, both in cultured cells and in Drosophila embryos, where it activates...

...Chihcheng Tsai; Peter Gergen ABSTRACT The segmentation genes runt and hairy are required for the proper transcriptional regulation of the pair-rule gene fushi tarazu during the blastoderm stage of Drosophila embryogenesis. The expression of different fushi tarazu reporter genes was examined in runt...

... to identify it unambiguously, but short conserved elements suggest a relationship to the segmentation gene fushi-tarazu , ( ftz ). We term it Sg Dax (divergent Antenna-pedia class homeobox gene). Antibodies raised against the protein encoded by this gene reveal two phases of expression during embryogenesis...

... of fushi tarazu in the posterior half of the embryo, it should not be expressed in the anterior half prior to gastrulation, and thus suggest a role for the CAD gradient. Ectopic expression of CAD at later stages of development has no obvious effects on embryogenesis or imaginai disc development, suggesting...

... segmentation gene, A . With antibodies, we than look at the expression of another gene that is known to be downstream of gene A, with respect to the cells in the patch. We have examples of patches of hairy cells (where we monitor the effect on fushi tarazu (ftz) expression), even-skipped (monitoring ftz...

...Timothy L. Karr; Thomas B. Kornberg The fushi tarazu (ftz) gene is essential for segmentation of the Drosophila embryo. This requirement is reflected at the cellular blastoderm stage of embryogenesis by seven transverse stripes of ftz expression. These stripes correspond to the missing segments...

...Peter A. Lawrence; Paul Johnston ABSTRACT The first sign of metamerization in the Drosophila embryo is the striped expression of pair-rule genes such as fushi tarazu ( ftz ) and even-skipped (eve). Here we describe, at cellular resolution, the development of ftz and eve protein stripes in staged...

...Lorenzo Magrassi; Peter A. Lawrence ABSTRACT The pair-rule mutant, fushi tarazu , causes deletion of alternate metameres. Here we show that there is cell death in the mutant which begins at the completion of germ band extension. We map the dying cells in the epidermis; they occur scattered all over...