... ( gsc ), chordin ( chd ) and floating head ( flh ) and in the formation of secondary axes. The same phenotypes are observed when a VP16-activator construct is injected into early embryos, indicating that eomes acts as a transcriptional activator. In addition, a dominant-negative construct and antisense...

...Elise Cau; Stephen W. Wilson The homeodomain transcription factor Floating head (Flh ) is required for the generation of neurones in the zebrafish epiphysis. It regulates expression of two basic helix loop helix (bHLH) transcription factor encoding genes, ash1a (achaete/scute homologue 1a...

... lineage markers gata1 and gata2 in the most posterior lateral plate mesoderm. Finally, we show that HrT is required for vasculogenesis in the trunk, leading to similar vascular defects to those observed in midline mutants such as floating head. hrT expression in the vascular progenitors depends upon...

... does not remove all goosecoid - and floating head -expressing cells, suggesting that the morphological shield does not comprise the entire organizer region. Complete removal of the embryonic shield and adjacent marginal tissue, however, leads to a loss of both prechordal plate and notochord...

... that the floating head homeobox gene is required in axial mesoderm to repress the expression of both spadetail and papc , promoting notochord and blocking differentiation of paraxial mesoderm. The PAPC structural cell-surface protein may provide a link between regulatory transcription factors and the actual cell...

...Sharon L. Amacher; Charles B. Kimmel ABSTRACT Cell fate decisions in early embryonic cells are controlled by interactions among developmental regulatory genes. Zebrafish floating head mutants lack a notochord; instead, muscle forms under the neural tube. As shown previously, axial mesoderm...

... of dorsal myotome for normal CaP pathfinding diminished over time. This process appears to depend on signals from the embryo, since dorsal myotomes matured in vitro remained permissive for CaP axons. Genetic mosaics between wild-type and floating head mutant embryos revealed notochord involvement in dorsal...

.... Weinberg; Christiane Nüsslein-Volhard ABSTRACT In a large scale screen for mutants with defects in the embryonic development of the zebrafish we identified mutations in four genes, floating head ( flh ), momo ( mom ), no tail ( ntl ), and doc , that are required for early notochord formation. Mutations...

... midline, in which most cells generate clones that develop exclusively as notochord. The borders of the notochord domain are sharp at the level of single cells, and coincide almost exactly with the border of the expression domain of the homeobox gene floating head ( flh ; zebrafish homologue of Xnot...

...M. E. Halpern; C. Thisse; R. K. Ho; B. Thisse; B. Riggleman; B. Trevarrow; E. S. Weinberg; J. H. Postlethwait; C. B. Kimmel ABSTRACT Zebrafish floating head mutant embryos lack notochord and develop somitic muscle in its place. This may result from incorrect specification of the notochord domain...