... in cardiomyocyte cell cycle arrest, we conditionally activated ROCK2 kinase domain (ROCK2:ER) in the murine postnatal heart. Here, we show that α5/β1 integrin and fibronectin matrix increase in response to actomyosin-mediated tension. Moreover, activation of ROCK2:ER promotes nuclear translocation of Yap...

... and more spermatogonia, and fibronectin was not detected in their basement membrane. The expression levels of genes encoding smooth muscle contractile proteins, Acta2 and Cnn1 , were downregulated in the smooth muscle cells lacking Hic1 , and the seminiferous tubules appeared to have reduced contractility...

... gastrulation accompanied by a VANGL planar cell polarity protein 2 (Vangl2)-regulated increase in filopodia number and polarization. Manipulation of blebs is sufficient to interfere with PCP and directed migration. We show that Vangl2, fibronectin and cadherin 2 function to suppress blebbing. Vangl2 maintains...

... activities in migrating gastrula cells and whether these processes require the fibronectin extracellular matrix. Here, we report that Vangl2 modulates the formation and polarization of actin-rich filopodia-like and large lamellipodia-like protrusions in ectodermal cells. By contrast, disrupted Glypican4/PCP...

...Martina Nagel; Rudolf Winklbauer ABSTRACT The leading-edge mesendoderm (LEM) of the Xenopus gastrula moves as an aggregate by collective migration. However, LEM cells on fibronectin in vitro show contact inhibition of locomotion by quickly retracting lamellipodia upon mutual contact. We found...

...Pooja R. Sonavane; Chong Wang; Bette Dzamba; Gregory F. Weber; Ammasi Periasamy; Douglas W. DeSimone The coordination of individual cell behaviors is a crucial step in the assembly and morphogenesis of tissues. Xenopus mesendoderm cells migrate collectively along a fibronectin (FN) substrate...

...-of-function assays (via microinjection of antisense morpholino or CRISPR-Cas9) confirm that AChE is specifically required in the gut endoderm tissue, a non-neuronal cell population, where it mediates adhesion to fibronectin and regulates cell rearrangement events that drive gut lengthening and digestive...

...Yuki Sato; Kei Nagatoshi; Ayumi Hamano; Yuko Imamura; David Huss; Seiichi Uchida; Rusty Lansford ABSTRACT Cells may exchange information with other cells and tissues by exerting forces on the extracellular matrix (ECM). Fibronectin (FN) is an important ECM component that forms fibrils through cell...

...Xia Wang; Sophie Astrof The chemical and mechanical properties of extracellular matrices (ECMs) modulate diverse aspects of cellular fates; however, how regional heterogeneity in ECM composition regulates developmental programs is not well understood. We discovered that fibronectin 1 ( Fn1...

... precardiac mesoderm formation in mouse embryonic stem cells and involved endodermal production of fibronectin. In vivo , fibronectin deficiency resulted in a dramatic reduction of mesoderm accompanied by endodermal expansion in zebrafish embryos. This event was mediated by regulation of Wnt signaling...

... movement. Gene expression profiles reveal heightened expression of fibronectin 1 ( fn1 ) in hand2 mutant embryos. Reciprocally, overexpression of hand2 leads to decreased Fibronectin levels. Furthermore, reduction of fn1 function enables rescue of cardiac fusion in hand2 mutants: bilateral cardiomyocyte...

...Arjan van der Flier; Kwabena Badu-Nkansah; Charles A. Whittaker; Denise Crowley; Roderick T. Bronson; Adam Lacy-Hulbert; Richard O. Hynes Integrin cell adhesion receptors and fibronectin, one of their extracellular matrix ligands, have been demonstrated to be important for angiogenesis using...

... (Sdc2) expressed in the extra-embryonic yolk syncytial layer (YSL) acts locally at the YSL-embryo interface to direct organ primordia migration, and is required for fibronectin and laminin matrix assembly throughout the embryo. Surprisingly, neither endogenous nor exogenous sdc2 expressed in embryonic...

... of Fibronectin (FN) depends on the FN receptor Integrinα5β1. Using in vivo imaging and genetic mosaics, our studies suggest that incipient Itgα5 clustering along the nascent border precedes matrix formation and is independent of FN binding. Integrin clustering can be initiated by Eph/Ephrin signaling...

... of fibronectin and tenascin-C, two well-known ligands of integrins. In vitro, tenascin-C and fibronectin have opposite effects on ENCCs, with tenascin-C decreasing migration and adhesion and fibronectin strongly promoting them. Itgb1 -null ENCCs exhibited an enhanced response to the inhibitory effect of tenascin...

... function locally promotes basement membrane dissolution in the primary mouth primordium. Consistently, Frzb-1 overexpression decreases RNA levels of the essential basement membrane genes fibronectin and laminin ,whereas Wnt-8 overexpression increases the levels of these RNAs. These data are the first...

..., and thus are both necessary for subsequent feather morphogenesis. BMP7 is expressed earlier and functions as a chemoattractant that recruits cells into the condensation, whereas BMP2 is expressed later, and leads to an arrest of cell migration,likely via its modulation of the EIIIA fibronectin domain...

... tubulogenesis (alveolarization). Lungs with mis/overexpressed Wnt5a develop with severe pulmonary hypoplasia associated with altered expression patterns of Shh , L-CAM,fibronectin, VEGF and Flk1 . This hypoplastic phenotype is rescued by either replacement of the Shh protein or inhibition of fibronectin...

.... Here we show in chick that the standard enzymatic treatments used for explant isolation destroy the fibronectin matrix surrounding the anterior PSM, which fails to form somites when cultured for 6 hours. By contrast, explants isolated with collagenase retain their fibronectin matrix and form somites...

... in the extra-embryonic YSL appears to regulate the embryonic expression of fibronectin , a gene previously implicated in myocardial migration. We further show dosage-sensitive genetic interactions between mtx1 and fibronectin . Based on these data, we propose that the extra-embryonic YSL regulates myocardial...