...Maureen Cetera; Rishabh Sharan; Gabriela Hayward-Lara; Brooke Phillips; Abhishek Biswas; Madalene Halley; Evalyn Beall; Bridgett vonHoldt; Danelle Devenport ABSTRACT The planar cell polarity (PCP) pathway collectively orients cells with respect to a body axis. Hair follicles of the murine epidermis...

...Susanne C. Baess; Ann-Kathrin Burkhart; Sabrina Cappello; Annika Graband; Kristin Seré; Martin Zenke; Catherin Niemann; Sandra Iden ABSTRACT The barrier-forming, self-renewing mammalian epidermis comprises keratinocytes, pigment-producing melanocytes and resident immune cells as first-line host...

... morphology during development, and the Myosin Vb-Rab10 interaction is essential for directional cell growth downstream of mTOR signaling. Cell size regulation Compensatory cell growth Tissue homeostasis Myosin Vb mTOR signaling Epidermis Zebrafish Tata Institute of Fundamental Research...

... and dynamics of transmembrane PCP proteins: Celsr1, Fz6 and Vangl2. Using the skin epidermis as a model, we characterize and verify the expression, localization and function of endogenously tagged Celsr1-3xGFP, Fz6-3xGFP and tdTomato-Vangl2 fusion proteins. Live imaging of Fz6-3xGFP in basal epidermal...

...Dimitris Katsanos; Mar Ferrando-Marco; Iqrah Razzaq; Gabriel Aughey; Tony D. Southall; Michalis Barkoulas ABSTRACT The epidermis of Caenorhabditis elegans is an essential tissue for survival because it contributes to the formation of the cuticle barrier as well as facilitating developmental...

... differentiation, induced extensive transcriptional reprogramming in the colonic epithelium and enhanced Apc -initiated adenoma development. The Ets DNA-binding domain of EHF is therefore essential for postnatal homeostasis of the epidermis and colonic epithelium, and its loss promotes colonic tumour development...

... stiffness decreased in det3 and clv3 det3 epidermises. Thus, we hypothesized that stem integrity depends on the epidermal resistance to mechanical stress. To formally test this hypothesis, we used the DET3 gene as part of a tissue-specific strategy to complement cell expansion defects. Epidermis-driven DET3...

...Kenji Nagata; Toshiki Ishikawa; Maki Kawai-Yamada; Taku Takahashi; Mitsutomo Abe ABSTRACT The differentiation of distinct cell types in appropriate patterns is a fundamental process in the development of multicellular organisms. In Arabidopsis thaliana , protoderm/epidermis differentiates...

... required to establish PCP are poorly understood. Here, we identified a major role for the globular (G)-actin-binding protein thymosin-β4 (TMSB4X) in PCP establishment and cell adhesion in the developing epidermis. Depletion of Tmsb4x in mouse embryos hindered eyelid closure and hair-follicle angling owing...

...Pooja Flora; Elena Ezhkova ABSTRACT Cell divisions and cell-fate decisions require stringent regulation for proper tissue development and homeostasis. The mammalian epidermis is a highly organized tissue structure that is sustained by epidermal stem cells (ESCs) that balance self-renewal and cell...

... show that epidermis-specific ablation of Nir impairs epidermal stratification and barrier function, resulting in perinatal lethality. Nir-deficient epidermis lacks appendages and remains single layered during embryogenesis. Cell proliferation is inhibited, whereas apoptosis and p53 acetylation...

... to P4. In brief, skins were removed, put on sterile 3MM paper and trypsinized (EDTA-free 0.25% trypsin) for 16 h at 4°C to separate the epidermis from the dermis. Epidermises were minced, and cells were detached by stirring on ice for 1 h. Cell suspensions were filtered and seeded on dishes coated...

... by a novel cytoplasmic poly A-binding protein, SMED-PABPC2. Knockdown of smed-pabpc2 leads to defects in epidermal lineage specification, disorganization of epidermis and ECM, and deregulated wound healing, resulting in the selective failure of neoblast proliferation near the wound region. Polysome profiling...

...Hsing-Jung Wu; Ji Won Oh; Dan F. Spandau; Sunil Tholpady; Jesus Diaz, III; Laura J. Schroeder; Carlos D. Offutt; Adam B. Glick; Maksim V. Plikus; Sachiko Koyama; John Foley Maintenance of specialized epidermis requires signals from the underlying mesenchyme; however, the specific pathways involved...

...Kaoru S. Imai; Hiroki Hikawa; Kenji Kobayashi; Yutaka Satou Epidermis and neural tissues differentiate from the ectoderm in animal embryos. Although epidermal fate is thought to be induced in vertebrate embryos, embryological evidence has indicated that no intercellular interactions during early...

..., strong correlation between ploidy and cell volume in pavement cells of the epidermis, this correlation was extremely weak in palisade mesophyll cells. Induction of epidermis cell identity based on the expression of the homeobox gene ATML1 in mesophyll cells enhanced the level of correlation between...

... of which remains unclear. We found that genotypes impaired in the TONNEAU1a ( TON1a ) gene display misoriented symmetric divisions in the epidermis and have no division defects in the underlying cortical tissue. The TON1a gene encodes a microtubule-associated protein. We show that in the ton1a mutant...

... are poorly understood. Using the developing Xenopus epidermis and regenerating human upper airways, we reveal the importance of BMP signalling for the construction of vertebrate mucociliary epithelia. In Xenopus , attenuation of BMP activity is necessary for the specification of multiciliated cells (MCCs...

..., endoreduplication and the acquisition of differentiated cellular morphologies. Here we show that the Arabidopsis phytocalpain DEFECTIVE KERNEL 1 (DEK1) promotes the differentiated epidermal state. Plants with reduced DEK1 activity produce cotyledon epidermis with protodermal characteristics, despite showing normal...

...Troels Schepeler; Mahalia E. Page; Kim B. Jensen The epidermis is an integral part of our largest organ, the skin, and protects us against the hostile environment. It is a highly dynamic tissue that, during normal steady-state conditions, undergoes constant turnover. Multiple stem cell populations...