...Alexandra L. Joyner; João Ramalho Ortigão-Farias; Thomas Kornberg ABSTRACT More than 40 years ago, studies of the Drosophila engrailed and Hox genes led to major discoveries that shaped the history of developmental biology. We learned that these genes define the state of determination of cells...

... enhancer responsible for dpp expression in imaginal discs. Our data demonstrate that Engrailed, a homeodomain-containing transcription factor that serves as a cap cell marker, binds to this region and regulates dpp expression in cap cells. Further data suggest that En forms a complex with Nejire (Nej...

... expression – the prospective anterior and posterior ocelli – and the intervening interocellar domain. Our results highlight the role of the homeodomain transcription factor engrailed ( en ) both as a target and as a transcriptional repressor of hh signaling in the prospective interocellar region. Furthermore...

... induces neural apoptosis and extinction of MHB markers, which are rescued by re-expression of engrailed 2a ( eng2a ), an evolutionarily conserved target of the Grhl family. Co-injection of sub-phenotypic doses of grhl2b and eng2a morpholinos reproduces the apoptosis and MHB marker loss, but fails...

...Olivier Stettler; Rajiv L. Joshi; Andrea Wizenmann; Jürgen Reingruber; David Holcman; Colette Bouillot; François Castagner; Alain Prochiantz; Kenneth L. Moya Engrailed 1 and engrailed 2 homeoprotein transcription factors (collectively Engrailed) display graded expression in the chick optic tectum...

.... In vertebrates, several homeoproteins have also been shown to have direct non-cell-autonomous activities in the developing nervous system. We present the first in vivo evidence for homeoprotein signaling in Drosophila . Focusing on wing development as a model, we first demonstrate that the homeoprotein Engrailed...

... and for transcription of a core set of midline glial-expressed genes. The Hedgehog morphogen is secreted from ectodermal cells adjacent to the CNS midline and directs a subset of midline glia to become PMG. Two transcription factor genes, runt and engrailed , play important roles in AMG and PMG development. The runt...

.... * Author for correspondence ( [email protected] ) Competing interests statement 3 12 2010 © 2011. BMP Smad BiFC Engrailed Patched Gli Sonic Hedgehog Myotome Muscle pioneers Zebrafish Members of the Hedgehog (Hh) family of signalling...

...Morgane Dolez; Jean-François Nicolas; Estelle Hirsinger In zebrafish, Hedgehog-induced Engrailed expression defines a muscle fibre population that includes both slow and fast fibre types and exhibits an organisational role on myotome and surrounding tissues, such as motoneurons and lateral line...

...Caroline C. G. Fabre; José Casal; Peter A. Lawrence The abdomen of adult Drosophila bears mechanosensory bristles with axons that connect directly to the CNS, each hemisegment contributing a separate nerve bundle. Here, we alter the amount of Engrailed protein and manipulate the Hedgehog signalling...

... identified on the basis that they express the homeobox gene engrailed ( en ). For each lineage,we determine the total number of adult-specific neurons generated as well as number and pattern of en -expressing cells. We then demonstrate that programmed cell death has a pronounced effect on the number of cells...

... repression of Ubx transcription. Similar results are obtained with the gene engrailed , showing that this mechanism of permanent repression may be a general one for genes with negative auto-regulation when levels of expression are transitorily elevated. * Author for correspondence (e-mail: esherrero...

..., a combination of repression by Wingless and activation by Hedgehog induces expression of the proneural gene lethal of scute in the most anterior midline daughter cells of the neighbouring posterior segment. Hedgehog and Lethal of scute activate Engrailed in these anterior cells. Engrailed-positive midline cells...

...Giuseppe Cassata; Gidi Shemer; Paolo Morandi; Roland Donhauser; Benjamin Podbilewicz; Ralf Baumeister engrailed is a homeobox gene essential for developmental functions such as differentiation of cell populations and the onset of compartment boundaries in arthropods and vertebrates. We present...

... for correspondence (e-mail: [email protected] ) 19 5 2004 © 2004. 2004 Theromyzon rude Leech Nervous system Cell lineage engrailed The leech nervous system comprises a relatively simple network of longitudinal and segmentally iterated transverse nerves, yet provides...

... medial muscle pioneer cells and migratory superficial slow fibres ( Devoto et al.,1996 ). In Xenopus , we do not find medially located pioneer-like slow fibres. Although widespread Engrailed 1-like immunoreactivity was reported in tail somites( Davis et al., 1991 ), we observed no En1 mRNA in tail...

... ) and engrailed ( en ). However, in the case of en the combination of Runt +Ftz gives activation. The contrasting responses of different downstream targets to Runt in the presence or absence of Ftz is thus central to the combinatorial logic of the pair-rule to segment-polarity transition. The unique and simple...

...Camilla W. Larsen; Elizabeth Hirst; Cyrille Alexandre; Jean-Paul Vincent In Drosophila embryos, segment boundaries form at the posterior edge of each stripe of engrailed expression. We have used an HRP-CD2 transgene to follow by transmission electron microscopy the cell shape changes that accompany...

... Engrailed Otx2 Swaying Chick-quail chimera Mouse The adult cerebellar cortex is subdivided mediolaterally into biochemically distinct functional subdomains organized into sagittal stripes or patches that may be considered as functional equivalents of the cortical areas of the mammalian cerebral...

... the establishment and maintenance of the diencephalic-mesencephalic boundary (DMB). We show that maintenance of the DMB requires both the presence of a specified midbrain and a functional MHB organizer. Expression of pax6.1 , a key regulator of forebrain development, is posteriorly suppressed by the Engrailed...