... diseases. However, how endogenous factors influence the proliferation and differentiation of intestinal stem cells (ISCs) under physiological and pathological conditions remains poorly understood. Here, we find that the evolutionarily conserved endoplasmic reticulum membrane protein complex (EMC...

...Katherine R. Rollins; J. Todd Blankenship ABSTRACT The endoplasmic reticulum (ER) undergoes a remarkable transition in morphology during cell division to aid in the proper portioning of the ER. However, whether changes in ER behaviors modulate mitotic events is less clear. Like many animal embryos...

... are observed when Vps13 is depleted from nurse cells but not from follicle cells. Microscopic analysis revealed enrichment of Vps13 in close proximity to the plasma membrane and the endoplasmic reticulum in nurse cells undergoing degradation. Ultrastructural analysis uncovered the presence of an underlying...

... perturb this process and have helped identify genes that affect progression of type 2 diabetes. Insulin maturation is required for its proper secretion by pancreatic β cells. The role of the endoplasmic reticulum (ER) chaperones in insulin processing and secretion needs further study. We show that the C...

...-A) is required for correction of surfactant structural and functional defects in the SP-A null mouse. J. Biol. Chem. 276 , 38542 -38548. Lomax, R. B., Camello, C., Van Coppenolle, F., Petersen, O. H. and Tepikin, A. V. ( 2002 ). Basal and physiological Ca 2+ leak from the endoplasmic reticulum...

... of the unfertilized egg, and within 8 μm of the surface the vegetal region and then posterior region of the zygote. By isolating cortices from eggs and zygotes we demonstrated that this reticulated structure is a network of cortical rough endoplasmic reticulum (cER) that is tethered to the plasma membrane...

... production is required to sustain the activity of the meiotic Ca 2+ wave pacemaker PM2. † Author for correspondence (e-mail: [email protected] ) 15 11 2002 © 2003. 2003 Fertilization Respiration Ca 2+ waves Mitochondria Endoplasmic reticulum ATP Ca 2+ wave pacemakers...

... cellular activities, many are involved in the assembly or disassembly of proteins or protein complexes; thus, OOC-5 may function as a chaperone. OOC-5 protein co-localizes with a marker of the endoplasmic reticulum in all blastomeres of the early C. elegans embryo, in a pattern indistinguishable from...

.... ooc - 3 is required for the correct localization of PAR-2 and PAR-3 cortical domains after the first cell division. OOC-3 is a novel putative transmembrane protein, which localizes to a reticular membrane compartment, probably the endoplasmic reticulum, that spans the whole cytoplasm and is enriched...

... to a polylysine-coated surface. The isolated cortex is further characterized by an elaborate network of tubules and sheets of endoplasmic reticulum (ER). This cortical ER network is tethered to the plasma membrane at discrete sites, is covered with ribosomes and contains a calse-questrin-like protein...

... grant no. BBS-8714235 to the Univ, of Massachusetts Microscopy Center. The authors would like to thank E. Sheldon for teaching M.M.M. the confocal microscope system. References Allen , N. S. and Brown , D. T. ( 1988 ). Dynamics of the endoplasmic reticulum in living onion epidermal...

...Bernard Maro; Martin H. Johnson; Susan J. Pickering; Daniel Louvard ABSTRACT The unfertilized oocyte, fertilized egg and early embryo (2-cell to 16-cell) of the mouse have been examined immunocytochemically for the distribution of antigens associated with the endoplasmic reticulum, the lysosomal...