... in the size of the nucleolus, the morphology of the nuclear envelope and the disposition of the chromatin within the nucleus, leading to a failure in the synthesis of ecdysone. We propose that loss of ecdysone production in the prothoracic gland of Spalt mutants is primarily caused by defects in nuclear pore...

... require the hormone ecdysone (Ec) for growth but require a drop in circulating Ec levels to regenerate. Examining Ec dynamics more closely, we find that transcriptional activity of the Ec-receptor (EcR) drops in uninjured regions of wing discs, but simultaneously rises in cells around the injury-induced...

... the niche. We find that GSC abscission failure is caused by increased Ecdysone hormone signaling induced upon mating, which leads to disrupted somatic encystment of the germline. Abscission failure is rescued by isolating males from females, but recurs with resumption of mating. Importantly, reiterative...

... of the third instar. This change in barrier permeability is driven by the steroid hormone ecdysone and correlates with changes in localization of Coracle, a component of the septate junctions that is required for the late-larval impermeable epithelial barrier. Based on these observations, we propose...

...: This study investigates the molecular keys that regulate the disintegration of the prothoracic gland during insect metamorphosis, after adulthood is reached. Prothoracic gland E93 FTZ-F1 Ecdysone Insect metamorphosis MEKRE93 pathway One of the most successful innovations in insect...

... identify the Ecdysone Receptor (EcR) in the PTTH-expressing neurons (PTTHn) as a regulator of developmental maturation onset. Loss of EcR in these PTTHn impairs PTTH signaling, which delays maturation. We find that the steroid ecdysone dose-dependently affects Ptth transcription, promoting its expression...

... in the same cells to respond to spatial cues through regulation of chromatin accessibility. Temporal transcription factor Chromatin accessibility Enhancer Gene regulation Ecdysone Drosophila National Institutes of Health T32GM007092 National Science Foundation 10.13039...

...Coralie Drelon; Michael F. Rogers; Helen M. Belalcazar; Julie Secombe ABSTRACT In Drosophila , the larval prothoracic gland integrates nutritional status with developmental signals to regulate growth and maturation through the secretion of the steroid hormone ecdysone. While the nutritional signals...

... to pupal stages but the expression of Fos is specifically activated by ecdysone receptor B1 (EcRB1) at early pupal stages, suggesting that ecdysone signaling provides temporal control of the regulation of dendrite pruning by JNK signaling. Thus, our work not only identifies a novel pathway involved...

...)00420-3 Beckstead , R. B. , Lam , G. and Thummel , C. S. ( 2007 ). Specific transcriptional responses to juvenile hormone and ecdysone in Drosophila . Insect Biochem. Mol. Biol.   37 , 570 - 578 . 10.1016/j.ibmb.2007.03.001 Birse , R. T. , Söderberg , J. A. E...

...H. Frederik Nijhout; Emily Laub; Laura W. Grunert ABSTRACT The wing imaginal disks of Lepidoptera can be grown in tissue culture, but require both insulin and ecdysone to grow normally. Here, we investigate the contributions the two hormones make to growth. Ecdysone is required to maintain mitoses...

... by the duration of this phase, which is under neuroendocrine control. The neuropeptide prothoracicotropic hormone (PTTH) has been proposed to play a central role in controlling the length of the larval phase through regulation of ecdysone production, a steroid hormone that initiates larval molting...

... in the optic lobe during early larval stages by boosting symmetric self-renewing divisions while preventing differentiation. Neuroepithelium differentiation in late larvae requires the transcriptional silencing of chinmo by ecdysone, the main steroid hormone, therefore allowing coordination of neural stem cell...

... in Drosophila disrupts the Wingless, Hedgehog and Decapentaplegic signaling pathways . Development   131 , 1927 - 1938 . 10.1242/dev.01061 Brennan , C. A. , Ashburner , M. and Moses , K. ( 1998 ). Ecdysone pathway is required for furrow progression in the developing Drosophila eye...

... - with multiple cis -regulatory elements integrating nutritional and tissue-specific inputs. dFOXO Ecdysone Insulin receptor Retinoblastoma Transcriptional enhancer National Institutes of Health 10.13039/100000002 GM056976 The insulin signaling pathway plays essential roles...

... timing is believed to be determined by the release of a steroid hormone, ecdysone (E), from the prothoracic gland. Here, we demonstrate that the ecdysone-20-monooxygenase Shade determines pupation timing by converting E to 20-hydroxyecdysone (20E) in the fat body, which is the organ that senses...

... Summary: Quantifying the full complement of endogenous ecdysteroids in Drosophila provides insights into the metabolic pathways of ecdysteroid production, and how these vary with diet and development. Drosophila melanogaster LC-MS/MS Dietary sterols Ecdysone Ecdysteroids Makisterone...

... and germ cell differentiation, and promotes the accumulation of Broad-Z1, which is also a target of the steroid hormone ecdysone. Epistasis analysis shows that Activin controls cell proliferation in an ecdysone-independent manner and TF differentiation by affecting ecdysone targets. We propose...

... to fine-tune the dynamics of hormone production. In Drosophila , transient increases of the steroid prohormone ecdysone, produced at each larval stage, are necessary to trigger moulting and metamorphosis. Binding of the active ecdysone (20-hydroxyecdysone) to its receptor (EcR) is followed...

... coordinate growth and timing of maturation during development, the mechanisms generating these pulses are not known. Here we show that the ecdysone steroid pulse that drives the juvenile-adult transition in Drosophila is determined by feedback circuits in the prothoracic gland (PG), the major steroid...