.... Summary: Loss- and gain-of-function, and floor plate deletions, reveal that Shh that transits through the sclerotome is presented to the neuroepithelium from its basal aspect to affect motoneuron development. BMP Dermomyotome Dorso-ventral patterning Hb9 Motoneurons Myotome Neural tube Nkx...

... zebrafish as a model, we describe the in vivo dynamics and function of embryonic muscle progenitor cells (MPCs) in the dermomyotome. These cells are located in a superficial layer external to muscle fibers and express many extracellular matrix (ECM) genes, including collagen type 1 α2 ( col1a2 ). Utilizing...

... of Biologists Ltd 2017 Summary: This Review discusses the mechanisms that underpin the formation of skeletal muscle during development and its recapitulation from pluripotent stem cells in vitro . Skeletal myogenesis Muscle differentiation Paraxial mesoderm Somite Dermomyotome Muscular...

... + dermomyotome. Conditional ubiquitous expression indicates that Mesp-b acts by inhibiting myogenic differentiation and by inducing the dermomyotome marker meox1 . By contrast, Ripply1 induces a negative-feedback loop by promoting Tbx6 protein degradation. Persistent Tbx6 expression in Ripply1 knockdown embryos...

... 2015 Summary : Epithelial cells of chick embryo somites exhibit filopodia-like protrusions that serve as transport conduits connecting them to the overlying surface ectoderm. Somite Dermomyotome Filopodia FiLiPs Frizzled-7 The formation of a complex multicellular organism...

... ) Competing interests The authors declare no competing financial interests 6 1 2014 13 3 2014 © 2014. Published by The Company of Biologists Ltd 2014 Somite WNT Dermomyotome Chick embryo Somites are blocks of cells sequentially generated through the epithelialization...

... The authors declare no competing financial interests. 29 4 2013 © 2013. Published by The Company of Biologists Ltd 2013 Fin mesenchyme Dermomyotome Ectomesenchyme Fibroblast Neural crest The neural crest is a multipotent embryonic stem cell population formed during...

... and differentiation into fibers, demonstrating that regulation of pioneer patterning is somite-intrinsic. Furthermore, pioneer myoblasts express Robo2 downstream of MyoD and Myf5, whereas the dermomyotome and caudal sclerotome express Slit1. Loss of Robo2 or of sclerotome-derived Slit1 function perturbed both...

...Frank Stellabotte; Betsy Dobbs-McAuliffe; Daniel A. Fernández; Xuesong Feng; Stephen H. Devoto The myogenic precursors responsible for muscle growth in amniotes develop from the dermomyotome, an epithelium at the external surface of the somite. In teleosts, the myogenic precursors responsible...

... and the epithelial somites, Wnt6 is expressed in the entire ectoderm overlying the neural tube, the paraxial mesoderm and the lateral plate mesoderm. With somite maturation, expression becomes restricted to the lateral ectoderm covering the ventrolateral lip of the dermomyotome and the lateral plate mesoderm...

.... Furthermore, inhibition ofα6β1 resulted in an increase in Myf5 protein and ectopic myogenin expression in dermomyotomal cells, suggesting that α6β1-laminin interactions normally repress myogenesis in the dermomyotome. We conclude that Myf5 is required for maintaining α6β1 expression on myogenic precursor...

...Yuval Cinnamon; Raz Ben-Yair; Chaya Kalcheim Myotomal fibers form by a first wave of pioneer myoblasts from the medial epithelial somite, and by a second wave from all four lips of the dermomyotome. Then, a third wave of mitotic progenitors colonizes the myotome,initially stemming from the extreme...

... the myogenic determination genes XMyf5 , XMyoD and Pax3 in a pattern reminiscent of amniote dermomyotome. Thus, Xenopus somites have characteristics in common with both fish and amniotes that shed light on the evolution of somite differentiation. We propose a model for the evolutionary adaptation of myogenesis...

...Raz Ben-Yair; Nitza Kahane; Chaya Kalcheim We have previously shown that overall growth of the myotome in the mediolateral direction occurs in a coherent and uniform pattern. We asked whether development of the dermomyotome and resultant dermis follow a similar pattern or are, alternatively...

... and specification of dermal progenitors from the medial dermomyotome. This comprises two distinct domains: the dorsomedial lip and a more central region (or intervening zone) that derives from it. We confirm that Wnt1 induces Wnt11 expression in the dorsomedial lip as previously shown, and show using DiI injections...

..., indicating that there are significant changes in either the signalling environment or the responsiveness of the cells along the rostrocaudal axis. We propose that the first phase of Myf5 epaxial expression, driven by the early epaxial enhancer in the dermomyotome, is necessary for early myotome formation...

... of the dermomyotome but cells enter the myotome only from the rostral and caudal lips. We provide new evidence for the existence of these distinctive waves. We show for the first time that when the somite dissociates, pioneer myotomal progenitors migrate as mesenchymal cells from the medial side towards the rostral...

...Sophie Eloy-Trinquet; Jean-François Nicolas In vertebrates, muscles of the back (epaxial) and of the body wall and limbs (hypaxial) derive from precursor cells located in the dermomyotome of the somites. In this paper, we investigate the mediolateral regionalisation of epaxial and hypaxial muscle...

...Nitza Kahane; Yuval Cinnamon; Ido Bachelet; Chaya Kalcheim The myotome is formed by a first wave of pioneer cells originating from the entire dorsomedial region of epithelial somites and a second wave that derives from all four lips of the dermomyotome but generates myofibers from only the rostral...

... as compared to controls with Pax3 +/− ES cells. We propose that although Pax3 function is related to cell surface properties, its role may differ in various tissues. In fact, apoptosis was found in Pax3 -deficient cells of the lateral dermomyotome but not in the neural tube. *Author for correspondence (e...