... rates of ≈0.8 for both channels, roughly comparable to the performance observed in 2-plex experiments using bandpass HCR imaging ( Choi et al., 2018 ), indicating that spectral imaging and linear unmixing are compatible with single-molecule imaging in 10-plex experiments. Fig. 4. dHCR imaging...

... – analog mRNA relative quantitation for high-throughput expression profiling of mammalian and bacterial cells; and (3) dHCR imaging – digital mRNA absolute quantitation via single-molecule imaging in thick autofluorescent samples. * Author for correspondence ( [email protected] ) Competing...