... to minimize susceptibility to differentiation factors. Drosophila female germline stem cells (GSCs) have short G1 and long G2 phases, and diet-dependent systemic factors often modulate G2. We previously observed that Cyclin E (CycE), a known G1/S regulator, is atypically expressed in GSCs during G2/M; however...

... and meiotic cell fate. Mitotic cell cycle progression occurs rapidly, continuously, with little or no time spent in G1, and with cyclin E (CYE-1) levels and activity high throughout the cell cycle. In addition to driving mitotic cell cycle progression, CYE-1 and CDK-2 also play an important role...

... multiple proteins for degradation, including the G1-S regulator Cyclin E and the oncoprotein dMyc/c-Myc. Despite prominent roles in growth control, little is known about the signals that regulate Ago/Fbw7 abundance in developing tissues. Here we use the Drosophila eye as a model to identify developmental...

... by the APC/C-dependent oscillation of the pre-replication complex component Orc1. Taken together, our data suggest a model in which the cyclic activity of APC/C Fzr/Cdh1 during the Drosophila endocycle is driven by the periodic inhibition of Fzr/Cdh1 by Cyclin E/Cdk2. We propose that, as is observed...

... phase. We have investigated the developmental regulation of Double-parked (Dup) protein, the Drosophila ortholog of Cdt1, a conserved and essential pre-RC component found in human and other organisms. We find that phosphorylation and degradation of Dup protein at G 1 /S requires cyclin E/CDK2. The N...

... the opposite effect. BrdU incorporation and Cyclin E protein levels continue to oscillate in dMyc-overexpressing cells, indicating that the normal cell cycle control mechanisms are not disrupted. dMyc driven growth and endoreplication are strongly attenuated when the endocycle is blocked with Cyclin E...

... to upregulation of Cyclin E in late GMC and its unequal distribution between two daughter cells. GMCs in an embryo overexpressing Cyclin E , or in an embryo mutant for archipelago , also undergo self-renewing asymmetric divisions. Although the GMC self-renewal is independent of inscuteable and numb , the fate...

[email protected] ) * These authors contributed equally to this work 20 10 2003 © 2004. 2004 Myc dmyc Proto-oncogene Nurse cells Follicle Egg chamber Gene amplification Dacapo Cyclin E Polyploidization Endoreduplication The ability of Myc to participate in these diverse...

..., Cyclin A persists longer than in wild type. In addition, Cyclin E expression is not downregulated after the fourth mitosis and accumulates in a polyubiquitinated form. Mutations in genes coding for components of the SCF pathway such as cul1, UbcD2 and effete enhance the extra division phenotype of encore...

... Dacapo that two modes of cell-cycle regulation are independently maintained within the common cytoplasm of ovarian cysts. * Author for correspondence (e-mail: [email protected] ) 20 12 2002 © 2003. 2003 Oogenesis Meiosis dacapo p27 Drosophila Cyclin E Oocyte...

..., the Drosophila RB family homolog, in cell cycle progression in the second mitotic wave. RBF-280, a mutant form of RBF that has four putative cdk phosphorylation sites mutated, can no longer be regulated by Cyclin D or Cyclin E. Expression of RBF-280 in the developing eye revealed that RBF-280 does not inhibit G1...

...Lynn Jones; Helena Richardson; Robert Saint ABSTRACT Cyclin E is an essential regulator of S phase entry. We have previously shown that transcriptional regulation of the gene that encodes Drosophila cyclin E, DmcycE , plays an important role in the control of the G1 to S phase transition during...

...David S. Fay; Min Han ABSTRACT We have identified strong loss-of-function mutations in the C. elegans cyclin E gene, cye-1 . Mutations in cye-1 lead to the underproliferation of many postembryonic blast lineages as well as defects in fertility and gut-cell endoreduplication. In addition, cye-1...

... for normal patterning and development. To determine how the embryo responds to hyperplastic growth, we have examined epidermal development in embryos that experience additional rounds of mitosis as the result of ectopic Cyclin E expression. We observed that the cell density in the epidermis nearly doubled...

...Helena Richardson; Louise V. O’Keefe; Thomas Marty ABSTRACT During animal development, cell proliferation is controlled in many cases by regulation of the G 1 to S phase transition. Studies of mammalian tissue culture cells have shown that the G 1 -specific cyclin, cyclin E, can be rate limiting...

...Robert J. Duronio; Patrick H. O’Farrell ABSTRACT We have defined a coordinate program of transcription of S-phase genes (DNA polymerase α, PCNA and the two ribonucleotide reductase subunits) that can be induced by the G 1 cyclin, cyclin E. In Drosophila embryos, this program drives an intricate...