...Brian Biehs; Katerina Kechris; SongMei Liu; Thomas B. Kornberg Paracrine Hedgehog (Hh) signaling regulates growth and patterning in many Drosophila organs. We mapped chromatin binding sites for Cubitus interruptus (Ci), the transcription factor that mediates outputs of Hh signal transduction...

...David Kent; Erik W. Bush; Joan E. Hooper The final step in Hedgehog (Hh) signal transduction is post-translational regulation of the transcription factor, Cubitus interruptus (Ci). Ci resides in the cytoplasm in a latent form, where Hh regulates its processing into a transcriptional repressor...

... repression of eya and decapentaplegic (dpp) expression by the zinc-finger transcription factor Cubitus interruptus (Ci rep). Furthermore, our results suggest that stabilized, full length Ci (Ci act) plays little or no role in Drosophila eye development. Moreover, while the effects of Hh are primarily...

... , and a repressor form of the transcription factor Cubitus interruptus can block decapentaplegic misexpression but not hedgehog misexpression. Loss of hyperplastic discs causes the accumulation of full-length Cubitus interruptus protein, but not of Smoothened, in both the eye and wing discs. hyperplastic discs...

...Wim G. M. Damen Spiders belong to the chelicerates, which is a basal arthropod group. To shed more light on the evolution of the segmentation process, orthologs of the Drosophila segment polarity genes engrailed , wingless/Wnt and cubitus interruptus have been recovered from the spider Cupiennius...

... for correspondence (e-mail: [email protected]) 24 7 2001 © 2001. 2001 groucho hedgehog cubitus interruptus Drosophila Wing patterning Repression Co-repressor The secreted proteins of the Hedgehog (Hh) family play a central role in the patterning of many structures...

... outputs of the pathway is still poorly understood. The Gli homolog Cubitus interruptus (Ci) is involved in controling the transcription of Drosophila Hh target genes and thus represents the most downstream component known in this pathway. We address the question of whether the Hh pathway is distally...

... by expressing rhomboid and patched . Here, we have examined the role of the transcription factor Cubitus interruptus (Ci) in this process. So far, Ci has been thought to be the most downstream component of the Hh pathway capable of activating all Hh functions. However, our current study of a null ci allele...

... homolog, cubitus interruptus (CI), is expressed only in the anterior compartment where it represses targets such as the signaling molecule genes decapentaplegic (dpp) and wingless (wg). HH is expressed in the posterior and diffuses into the anterior where it antagonizes CI repression resulting in dpp...

... limbs is initiated by hedgehog signaling from cells of the posterior half to cells of the anterior half of the limb primordium. Hedgehog signaling requires the anterior-specific expression of the gene cubitus interruptus to establish posterior-specific hedgehog secretion and anterior-specific competence...

...Nathalie Méthot; Konrad Basler ABSTRACT Hedgehog controls the expression of key developmental genes through the conversion of the transcription factor Cubitus interruptus (Ci) into either an activator (Ci[act]) or a repressor (Ci[rep]) form. Proteolytic cleavage of full-length Ci is important...

...Bruno Müller; Konrad Basler ABSTRACT The Drosophila Gli homolog Cubitus interruptus (Ci) controls the transcription of Hedgehog (Hh) target genes. A repressor form of Ci arises in the absence of Hh signalling by proteolytic cleavage of intact Ci, whereas an activator form of Ci is generated...

...Q. Tian Wang; Robert A. Holmgren ABSTRACT The Hedgehog (Hh) signal is transduced via Cubitus interruptus (Ci) to specify cell fates in the Drosophila wing. In the absence of Hh, the 155 kDa full-length form of Ci is cleaved into a 75 kDa repressor. Hh inhibits the proteolysis of full-length Ci...

... of or in parallel with Hedgehog signaling and (3) the Hedgehog signaling pathway required for Bolwig’s organ development is a new type and lacks Fused kinase and Cubitus interruptus as downstream components. * Author for correspondence (e-mail: [email protected]) 02 09 200 18 01 2000...

...Q. Tian Wang; Robert A. Holmgren Cubitus interruptus (Ci), a Drosophila transcription factor, mediates Hedgehog (Hh) signaling during the patterning of embryonic epidermis and larval imaginal discs. In the absence of Hh signal, Ci is cleaved to generate a truncated nuclear form capable...

...Mary Ann Price; Daniel Kalderon ABSTRACT The Hedgehog signal transduction pathway is involved in diverse patterning events in many organisms. In Drosophila , Hedgehog signaling regulates transcription of target genes by modifying the activity of the DNA-binding protein Cubitus interruptus (Ci...

... Wingless is in turn maintaining engrailed and hedgehog expression. By expressing different components of the Hedgehog pathway only in anterior, wingless -expressing cells we could show that the Hedgehog signalling components Smoothened and Cubitus interruptus are required in cells posterior to Hedgehog...

...Yang Chen; Jean-René Cardinaux; Richard H. Goodman; Sarah M. Smolik Hedgehog (HH) is an important morphogen involved in pattern formation during Drosophila embryogenesis and disc development. cubitus interruptus (ci) encodes a transcription factor responsible for transducing the hh signal...

... dpp enhancer element, the dpp heldout enhancer, from the 3′ cis regulatory disc region of the dpp locus. In this report, we present a molecular analysis of this 358 bp wing-and haltere-specific dpp enhancer, which demonstrates a direct transcriptional requirement for the Cubitus interruptus (Ci...

... clones and is specifically activated by exogenously supplied Cubitus interruptus (Ci). D/V- h expression is also lost in clones deficient for Dpp and Wg signaling, but ectopic activation of D/V- h by Dpp and Wg is limited to the A/P compartment boundary where endogenous levels of full- length Ci are high...