... key areas in which to refine our understanding of this signaling mechanism. * Author for correspondence ( [email protected] ) Competing interests The author declares no competing or financial interests. © 2021. Published by The Company of Biologists Ltd 2021...

... confirm that naïve PSCs do not respond directly to germ layer induction, but must first acquire competence. Capacitation for multi-lineage differentiation occurs without exogenous growth factor stimulation and is facilitated by inhibition of Wnt signalling. Whole-transcriptome profiling during...

...Pavel Vopalensky; Sabrina Pralow; Nadine L. Vastenhouw ABSTRACT The activation of specific gene expression programs depends on the presence of the appropriate signals and the competence of cells to respond to those signals. Although it is well established that cellular competence is regulated...

... to control photoreceptor cell fate specification. * Author for correspondence ( [email protected] ) Competing interests The authors declare no competing or financial interests. © 2015. Published by The Company of Biologists Ltd 2015 Summary: This Review discusses recent progress...

... genetic lineage tracing method, and genetic data, we show that the competence to embark on a neuronal or sensory fate is provided sequentially and very early during otic placode induction. Loss of Foxi1 prevents neuronal precursor formation without affecting hair cell specification, whereas loss of Dlx3b...

...Johnny J. Touma; Frank F. Weckerle; Michael D. Cleary Similar to mammalian neural progenitors, Drosophila neuroblasts progressively lose competence to make early-born neurons. In neuroblast 7-1 (NB7-1), Kruppel (Kr) specifies the third-born U3 motoneuron and Kr misexpression induces ectopic U3...

...Hidehiko Hashimoto; Takashi Enomoto; Gaku Kumano; Hiroki Nishida In embryos of the ascidian Halocynthia roretzi , the competence of isolated presumptive notochord blastomeres to respond to fibroblast growth factor (FGF) for induction of the primary notochord decays by 1 hour after cleavage from...

... in the absence of endogenous Hunchback. Previous studies have shown that Hunchback can specify early-born neuronal identity and maintain ‘young’ neural progenitor (neuroblast) competence. We identify two conserved domains required for Hunchback-mediated transcriptional repression, and show that transcriptional...

... tube and epidermis. Here, Dlx3, Dlx5, Pax6 and the pan-neuronal marker Hu serve as molecular labels to follow the maturation of olfactory precursors over time. When competence to form olfactory placode was tested by grafting ectoderm from different axial levels to the anterior neural fold, we found...

... Competence Genomics Induction Lens Notch Xenopus Although many signaling factors and transcriptional regulators are essential for organ formation in vertebrates, little is known about how these multiple inputs are integrated to generate the specific locations and identities of particular...

... origins in different tissues. Excess RA leads to ectopic foxi1 expression throughout the entire preplacodal domain. Foxi1 provides competence to adopt an otic fate. Subsequently, pax8 , the expression of which depends upon Foxi1 and Fgf, is also expressed throughout the preplacodal domain. By contrast...

... region when Bmp or Fgf signaling was blocked just before hepatic specification. However, hhex and prox1 expression in adjacent endodermal and mesodermal tissues appeared unaffected by these manipulations. Additional genetic studies indicate that the endoderm maintains competence for Bmp-mediated...

...-placodal domain. By contrast, ectoderm from outside this domain is not competent to express otic markers in response to Fgfs. Grafting naïve ectoderm into the pre-placodal domain causes upregulation of pre-placodal markers within 8 hours, together with the acquisition of competence to respond to Fgf...

...Andrew D. Chalmers; Bernhard Strauss; Nancy Papalopulu A key feature of early vertebrate development is the formation of superficial, epithelial cells that overlie non-epithelial deep cells. In Xenopus , deep and superficial cells show a range of differences,including a different competence...

... Muscle patterning Chick/quail Experimental embryology Somite Dermatome Competence Confocal microscopy Epaxial Stem cell Progenitor cell Asymmetry In conclusion, the results of the studies conducted here are consistent with the hypothesis that the dermomyotome DML contains a population...

... suggest that spg / pou2 is a transcription factor that mediates regional competence to respond to Fgf8 signaling. *Author for correspondence (e-mail: [email protected] ) 27 11 2001 © 2002. 2002 Competence Fgf8 pou2 Oct3/4 Pou5f1 MHB Isthmus pax2 spiel-ohne-grenzen...

... centers that pattern neighboring fields to create molecularly distinct domains. We investigated the mechanisms underlying the regionally distinct competence for two such organizing signals, Fibroblast growth factor 8 (Fgf8) and Sonic hedgehog (Shh), using chick embryos. First, we demonstrated that FGF...

...-hindbrain boundary (MHB) and for conferring competence to the anterior neuroectoderm in responding to forebrain-, midbrain- and rostral hindbrain-inducing activities. *Author for correspondence (e-mail: [email protected] ) 10 9 2001 © 2001. 2001 Otx2 Gbx2 Forebrain...

... specified to form the otic placode at the 4-6ss, and that this ectoderm is committed to a placodal fate by the 10ss. We also demonstrate that much of the embryonic ectoderm is competent to generate an otic placode if taken at a sufficiently early age. We have mapped the location of otic placode-inducing...

... formation of loose cell aggregates that may indicate neural crest precursor cells. Aggregates do not express an epidermal marker, indicating that opl suppresses ventral fates. Together, these data suggest that opl may mediate neural competence and may be involved in activation of midbrain, dorsal neural...